The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Versatile protein tagging in cells with split fluorescent protein
Open accessAbstract
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectively. The small size of FP11-tags enables a cost-effective and scalable way to insert them into endogenous genomic loci via CRISPR-mediated homology-directed repair. Tandem arrangement FP11-tags allows proportional enhancement of fluorescence signal in tracking intraflagellar transport particles, or reduction of photobleaching for live microtubule imaging. Finally, we show the utility of tandem GFP11-tag in scaffolding protein oligomerization. These experiments illustrate the versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications.
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Cites
Global analysis of protein localization in budding yeast
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Self-citationGlobal analysis of protein expression in yeast
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Self-citationCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Self-citationSpatially resolved, highly multiplexed RNA profiling in single cells
Chen, K. H., Boettiger, A. N., Moffitt, J. R., Wang, S., & Zhuang, X. (2015) · Science · doi:10.1126/science.aaa6090 · Cited by 2,969
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728
Self-citationEngineering complex synthetic transcriptional programs with CRISPR RNA scaffolds
Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, J. E., Qi, L. S., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.052 · Cited by 938
Self-citationConformational biosensors reveal GPCR signalling from endosomes
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Self-citationFluorescent fusion protein knockout mediated by anti-GFP nanobody
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Lundberg, E., & Borner, G. H. H. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0094-y · Cited by 591
OpenCell: Endogenous tagging for the cartography of human cellular organization
Cho, N. H., Cheveralls, K. C., Brunner, A.-D., Kim, K., Michaelis, A. C., Raghavan, P., Kobayashi, H., Savy, L., Li, J. Y., Canaj, H., et al. (2022) · Science · doi:10.1126/science.abi6983 · Cited by 567
“In future iterations, other split systems that contain compartment-specific signal sequences could be used to target organellar lumen ( 23 ).”
BackgroundSelf-citationA Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging
Specht, E. A., Braselmann, E., & Palmer, A. E. (2017) · Annual Review of Physiology · doi:10.1146/annurev-physiol-022516-034055 · Cited by 444
Liquid–liquid phase separation drives cellular function and dysfunction in cancer
Mehta, S., & Zhang, J. (2022) · Nature Reviews Cancer · doi:10.1038/s41568-022-00444-7 · Cited by 437
A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion
Zhang, M., Liu, L., Lin, X., Wang, Y., Li, Y., Guo, Q., Li, S., Sun, Y., Tao, X., Zhang, D., et al. (2020) · Cell · doi:10.1016/j.cell.2020.03.031 · Cited by 294
Metadata
- Authors
- Kamiyama, D., Sekine, S., Barsi-Rhyne, B., Hu, J., Chen, B., Gilbert, L. A., Ishikawa, H., Leonetti, M. D., Marshall, W. F., Weissman, J. S., & Huang, B.
- Venue
- Nature Communications
- Published
- 2016-03-18
- Volume
- 7
- Issue
- 1
- Pages
- 11046-11046
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2301195113
- Cited by
- 456
- References
- 8
- FWCI
- 21.71
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kamiyama, D., Sekine, S., Barsi-Rhyne, B., Hu, J., Chen, B., Gilbert, L. A., Ishikawa, H., Leonetti, M. D., Marshall, W. F., Weissman, J. S., & Huang, B. (2016). Versatile protein tagging in cells with split fluorescent protein. Nature Communications. https://doi.org/10.1038/ncomms11046
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