The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc
Open accessAbstract
Despite the high prevalence of intervertebral disc disease, little is known about changes in intervertebral disc cells and their regenerative potential with ageing and intervertebral disc degeneration. Here we identify populations of progenitor cells that are Tie2 positive (Tie2+) and disialoganglioside 2 positive (GD2+), in the nucleus pulposus from mice and humans. These cells form spheroid colonies that express type II collagen and aggrecan. They are clonally multipotent and differentiated into mesenchymal lineages and induced reorganization of nucleus pulposus tissue when transplanted into non-obese diabetic/severe combined immunodeficient mice. The frequency of Tie2+ cells in tissues from patients decreases markedly with age and degeneration of the intervertebral disc, suggesting exhaustion of their capacity for regeneration. However, progenitor cells (Tie2+GD2+) can be induced from their precursor cells (Tie2+GD2−) under simple culture conditions. Moreover, angiopoietin-1, a ligand of Tie2, is crucial for the survival of nucleus pulposus cells. Our results offer insights for regenerative therapy and a new diagnostic standard. Back pain and sciatica are often caused by intervertebral disc degeneration. Sakai and colleagues identify a subset of nucleus pulposus progenitor cells from the intervertebral disc and show that loss of these progenitor cells correlates with ageing and intervertebral disc degeneration.
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Cites
Magnetic Resonance Classification of Lumbar Intervertebral Disc Degeneration
Pfirrmann, C. W. A., Metzdorf, A., Zanetti, M., Hodler, J., & Boos, N. (2001) · Spine · doi:10.1097/00007632-200109010-00011 · Cited by 4,012
Tie2/Angiopoietin-1 Signaling Regulates Hematopoietic Stem Cell Quiescence in the Bone Marrow Niche
Arai, F., Hirao, A., Ohmura, M., Sato, H., Matsuoka, S., Takubo, K., Ito, K., Koh, G. Y., & Suda, T. (2004) · Cell · doi:10.1016/j.cell.2004.07.004 · Cited by 1,974
Degeneration of the intervertebral disc.
Urban, J. P. G., & Roberts, S. (2003) · Arthritis Research · doi:10.1186/ar629 · Cited by 1,295
Antoniou, J., Steffen, T., Nelson, F., Winterbottom, N., Hollander, A. P., Poole, R. A., Aebi, M., & Alini, M. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118884 · Cited by 1,045
Self-citationPreliminary Evaluation of a Scheme for Grading the Gross Morphology of the Human Intervertebral Disc
THOMPSON, J. P., PEARCE, R. H., SCHECHTER, M. T., ADAMS, M. E., TSANG, I. K. Y., & BISHOP, P. B. (1990) · Spine · doi:10.1097/00007632-199005000-00012 · Cited by 865
HISTOLOGY AND PATHOLOGY OF THE HUMAN INTERVERTEBRAL DISC
ROBERTS, S., EVANS, H., TRIVEDI, J., & MENAGE, J. (2006) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/00004623-200604002-00003 · Cited by 828
Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells
Jones, E. A., Kinsey, S. E., English, A., Jones, R. A., Straszynski, L., Meredith, D. M., Markham, A. F., Jack, A., Emery, P., & McGonagle, D. (2002) · Arthritis & Rheumatism · doi:10.1002/art.10696 · Cited by 612
Oh, H., Takagi, H., Suzuma, K., Otani, A., Matsumura, M., & Honda, Y. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.22.15732 · Cited by 499
The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain
Freemont, A. J. (2008) · Rheumatology · doi:10.1093/rheumatology/ken396 · Cited by 485
Novel Markers for the Prospective Isolation of Human MSC
BÜHRING, H., BATTULA, V. L., TREML, S., SCHEWE, B., KANZ, L., & VOGEL, W. (2007) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1392.000 · Cited by 454
Cited by
Novais, E. J., Tran, V. A., Johnston, S. N., Darris, K. R., Roupas, A. J., Sessions, G. A., Shapiro, I. M., Diekman, B. O., & Risbud, M. V. (2021) · Nature Communications · doi:10.1038/s41467-021-25453-2 · Cited by 440
Self-citationMesenchymal stem cells: Amazing remedies for bone and cartilage defects
Kangari, P., Talaei-Khozani, T., Razeghian-Jahromi, I., & Razmkhah, M. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-02001-1 · Cited by 286
Metadata
- Authors
- Sakai, D., Nakamura, Y., Nakai, T., Mishima, T., Kato, S., Grad, S., Alini, M., Risbud, M. V., Chan, D., Cheah, K. S. E., Yamamura, K.-I., Masuda, K., Okano, H., Ando, K., & Mochida, J.
- Venue
- Nature Communications
- Published
- 2012-12-11
- Volume
- 3
- Issue
- 1
- Pages
- 1264-1264
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1982678468
- Cited by
- 451
- References
- 31
- FWCI
- 13.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sakai, D., Nakamura, Y., Nakai, T., Mishima, T., Kato, S., Grad, S., Alini, M., Risbud, M. V., Chan, D., Cheah, K. S. E., Yamamura, K.-I., Masuda, K., Okano, H., Ando, K., & Mochida, J. (2012). Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc. Nature Communications. https://doi.org/10.1038/ncomms2226
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