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Cited by 451

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc

Open access
Sakai, Daisuke, Nakamura, Yoshihiko, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand V., Chan, Danny, Cheah, Kathryn S.E., Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji·2012·Nature Communications
DOI 10.1038/ncomms2226
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Abstract

Despite the high prevalence of intervertebral disc disease, little is known about changes in intervertebral disc cells and their regenerative potential with ageing and intervertebral disc degeneration. Here we identify populations of progenitor cells that are Tie2 positive (Tie2+) and disialoganglioside 2 positive (GD2+), in the nucleus pulposus from mice and humans. These cells form spheroid colonies that express type II collagen and aggrecan. They are clonally multipotent and differentiated into mesenchymal lineages and induced reorganization of nucleus pulposus tissue when transplanted into non-obese diabetic/severe combined immunodeficient mice. The frequency of Tie2+ cells in tissues from patients decreases markedly with age and degeneration of the intervertebral disc, suggesting exhaustion of their capacity for regeneration. However, progenitor cells (Tie2+GD2+) can be induced from their precursor cells (Tie2+GD2−) under simple culture conditions. Moreover, angiopoietin-1, a ligand of Tie2, is crucial for the survival of nucleus pulposus cells. Our results offer insights for regenerative therapy and a new diagnostic standard. Back pain and sciatica are often caused by intervertebral disc degeneration. Sakai and colleagues identify a subset of nucleus pulposus progenitor cells from the intervertebral disc and show that loss of these progenitor cells correlates with ageing and intervertebral disc degeneration.

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Metadata

Authors
Sakai, D., Nakamura, Y., Nakai, T., Mishima, T., Kato, S., Grad, S., Alini, M., Risbud, M. V., Chan, D., Cheah, K. S. E., Yamamura, K.-I., Masuda, K., Okano, H., Ando, K., & Mochida, J.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2012-12-11
Volume
3
Issue
1
Pages
1264-1264
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms2226
OpenAlex
W1982678468
Cited by
451
References
31
FWCI
13.92
Type
journal article
Language
en
Open access
hybrid

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