The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging
Open accessAbstract
Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging. Infrared fluorescent proteins offer advantages for deep in vivo imaging thanks to the tissue-penetrating properties of infrared light. Here, Yu et al. design a monomeric infrared fluorescent protein that, when combined with expression of haeme oxygenase in cells, shows improved performance for in vivoimaging of neurons and brain tumours.
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Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
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Branching out: mechanisms of dendritic arborization
Jan, Y.-N., & Jan, L. Y. (2010) · Nature Reviews Neuroscience · doi:10.1038/nrn2836 · Cited by 693
Self-citationIshii, N., Maier, D., Merlo, A., Tada, M., Sawamura, Y., Diserens, A., & Van Meir, E. G. (1999) · Brain Pathology · doi:10.1111/j.1750-3639.1999.tb00536.x · Cited by 569
Local generation of glia is a major astrocyte source in postnatal cortex
Ge, W.-P., Miyawaki, A., Gage, F. H., Jan, Y. N., & Jan, L. Y. (2012) · Nature · doi:10.1038/nature10959 · Cited by 507
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Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016) · Nature Communications · doi:10.1038/ncomms12405 · Cited by 312
Near-Infrared Fluorescent Proteins, Biosensors, and Optogenetic Tools Engineered from Phytochromes
Chernov, K. G., Redchuk, T. A., Omelina, E. S., & Verkhusha, V. V. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00700 · Cited by 264
Li, F., Sami, A., Noristani, H. N., Slattery, K., Qiu, J., Groves, T., Wang, S., Veerasammy, K., Chen, Y. X., Morales, J., et al. (2020) · Cell Metabolism · doi:10.1016/j.cmet.2020.08.015 · Cited by 147
Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life
Jensen, G. C., Janis, M. K., Nguyen, H. N., David, O. W., & Zastrow, M. L. (2024) · ACS Sensors · doi:10.1021/acssensors.3c02695 · Cited by 25
Metadata
- Authors
- Yu, D., Gustafson, W. C., Han, C., Lafaye, C., Noirclerc-Savoye, M., Ge, W.-P., Thayer, D. A., Huang, H., Kornberg, T. B., Royant, A., Jan, L. Y., Jan, Y. N., Weiss, W. A., & Shu, X.
- Venue
- Nature Communications
- Published
- 2014-05-15
- Volume
- 5
- Issue
- 1
- Pages
- 3626-3626
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2001589031
- Cited by
- 165
- References
- 5
- FWCI
- 11.55
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yu, D., Gustafson, W. C., Han, C., Lafaye, C., Noirclerc-Savoye, M., Ge, W.-P., Thayer, D. A., Huang, H., Kornberg, T. B., Royant, A., Jan, L. Y., Jan, Y. N., Weiss, W. A., & Shu, X. (2014). An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nature Communications. https://doi.org/10.1038/ncomms4626
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