Fourth generation biofuel from genetically modified algal biomass for bioeconomic development
Abstract
Biofuels from microalgae have promising potential for a sustainable bioeconomy. Algal strains' oil content and biomass yield are the most influential cost drivers in the fourth generation biofuel (FGB) production. Genetic modification is the key to improving oil accumulation and biomass yield, consequently developing the bioeconomy. This paper discusses current practices, new insights, and emerging trends in genetic modification and their bioeconomic impact on FGB production. It was demonstrated that enhancing the oil and biomass yield could significantly improve the probability of economic success and the net present value of the FGB production process. The techno-economic and socioeconomic burden of using genetically modified (GM) strains and the preventive control strategies on the bioeconomy of FGB production is reviewed. It is shown that the fully lined open raceway pond could cost up to 25% more than unlined ponds. The cost of a plastic hoop air-supported greenhouse covering cultivation ponds is estimated to be US 60,000$ /ha. The competitiveness and profitability of large-scale cultivation of GM biomass are significantly locked to techno-economic and socioeconomic drivers. Nonetheless, it necessitates further research and careful long-term follow-up studies to understand the mechanism that affects these parameters the most.
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Such genetic modifications define the transition towards fourth-generation concepts, targeting significantly improved biofuel yields per hectare, although specific quantitative projections remain under development.
“Such genetic modifications define the transition towards fourth-generation concepts, targeting significantly improved biofuel yields per hectare, although specific quantitative projections remain under development [118,166].”
From Biofuels, E-Fuels, and Waste-Derived Fuels: Advances, Challenges, and Future Directions (2025) · cites this paper as [166]
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Fourth-Generation Biofuels: Genetic Engineering for Sustainable Energy
Bhagat, S. P., Wagh, B. D., Satpute, S. M., Gunjal, M. B., Kasar, S. K., & Roylawar, P. B. (2025) · Clean Energy Production Technologies · doi:10.1007/978-981-96-4636-4_15 · Cited by 2 · see citing works
Recent innovations in sustainable algal biofuel production: a comprehensive review
Mohapatra, A., Guliani, E., Nandal, V., Solanki, M., Pathak, V. V., Banga, S., & Sharma, D. K. (2025) · Brazilian Journal of Chemical Engineering · doi:10.1007/s43153-025-00598-5 · Cited by 2 · see citing works
Biofuels production by biomass gasification
Gimžauskaitė, D., & Tamošiūnas, A. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-95076-3.00009-0 · Cited by 2 · see citing works
González–Cancelas, N., Vaca-Cabrero, J., Orive, A. C., & Garzón, D. A. Á. (2025) · Energies · doi:10.3390/en18030649 · Cited by 1 · see citing works
Alcohol Fuels for Sustainable Energy Solutions
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Biocrude production via hydrothermal liquefaction of biomass: Recent advances and trends
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Algal Photo Bioreactors: A Promising Technology for Wastewater Treatment
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Is Biomass Energy a Boon or Bane for Society: A Comprehensive Analysis
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Energy production from microalgae
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Algal biodiesel production for its utility as a third-generation fuel
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Microalgae Biomass Production: Cultivation Techniques and Applications
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Aritonang, D., Erfianti, T., Putri, R. A. E., Kartina, K., Ramadhana, S. D., Sadewo, B. R., Asiandu, A. P., & Suyono, E. A. (2026) · International Journal of Renewable Energy Development · doi:10.61435/ijred.2026.61637
- Authors
- Shokravi, H., Heidarrezaei, M., Shokravi, Z., Ong, H. C., Lau, W. J., Din, M. F. M., & Ismail, A. F.
- Affiliations
- University of Technology Malaysia (MY)
- Islamic Azad University, Science and Research Branch (IR)
- National Yunlin University of Science and Technology (TW)
- Corresponding author
- Ong, Hwai Chyuan
- ORCID
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2022-12
- Volume
- 360
- Pages
- 23-36
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W4306803825
- Cited by
- 49
- References
- 190
- Citations by year
- 2026: 8 · 2025: 23 · 2024: 11 · 2023: 7
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Bioenergy crop production and management
- Keywords
- fourth-generation biofuels, algal biomass, bioeconomy, biomass yield, techno-economic analysis, net present value, greenhouse cultivation, raceway ponds, socio-economic impacts
- MeSH terms
- Biofuels
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