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The fidelity of the ligation step determines how ends are resolved during nonhomologous end joining

Waters, Crystal A., Strande, Natasha T., Pryor, John M., Strom, Christina N., Mieczkowski, Piotr, Burkhalter, Martin D., Oh, Sehyun, Qaqish, Bahjat F., Moore, Dominic T., Hendrickson, Eric A., Ramsden, Dale A.·2014·Nature Communications
DOI 10.1038/ncomms5286PMC PMC4107315
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Crossref · 10.1038/ncomms5286

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  • NHEJ can lead to small nucleotide insertions or deletions (indels) in the DSB region, which in turn can originate a vast host of insertions, deletions, or frameshift mutations.

    “NHEJ can lead to small nucleotide insertions or deletions (indels) in the DSB region, which in turn can originate a vast host of insertions, deletions, or frameshift mutations [69,70].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [70]

    The Results (Effects of terminal mispairs on end processing; page unavailable) document deletions at NHEJ repair junctions, and Methods (Sequencing; page unavailable) report insertions, though they were rare in the tested substrates. These passages support the possibility of indels at a break, but the paper does not establish the claimed range of downstream mutations or discuss frameshifts. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 54

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Metadata

Authors
Waters, C. A., Strande, N. T., Pryor, J. M., Strom, C. N., Mieczkowski, P., Burkhalter, M. D., Oh, S., Qaqish, B. F., Moore, D. T., Hendrickson, E. A., & Ramsden, D. A.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2014-07-03
Volume
5
Issue
1
ISSN
2041-1723
DOI
10.1038/ncomms5286
PMC
PMC4107315
Cited by
78
References
4
Language
en
Licence

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