The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Large-volume en-bloc staining for electron microscopy-based connectomics
Open accessAbstract
Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.
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Cites
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Cited by
Dense connectomic reconstruction in layer 4 of the somatosensory cortex
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Connectomic comparison of mouse and human cortex
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Self-citationReconstruction of neocortex: Organelles, compartments, cells, circuits, and activity
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Metadata
- Authors
- Hua, Y., Laserstein, P., & Helmstaedter, M.
- Venue
- Nature Communications
- Published
- 2015-08-03
- Volume
- 6
- Issue
- 1
- Pages
- 7923-7923
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2254436645
- Cited by
- 328
- References
- 17
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
