The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix
Open accessAbstract
Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I’s Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Myofibroblasts and mechano-regulation of connective tissue remodelling
Tomasek, J. J., Gabbiani, G., Hinz, B., Chaponnier, C., & Brown, R. A. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm809 · Cited by 4,290
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Reinke, J. M., & Sorg, H. (2012) · European Surgical Research · doi:10.1159/000339613 · Cited by 1,898
Humphries, J. D., Byron, A., & Humphries, M. J. (2006) · Journal of Cell Science · doi:10.1242/jcs.03098 · Cited by 1,708
The extracellular matrix in development and morphogenesis: A dynamic view
Rozario, T., & DeSimone, D. W. (2010) · Developmental Biology · doi:10.1016/j.ydbio.2009.10.026 · Cited by 1,451
Balancing forces: architectural control of mechanotransduction
DuFort, C. C., Paszek, M. J., & Weaver, V. M. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3112 · Cited by 985
Cancer as an overhealing wound: an old hypothesis revisited
Schäfer, M., & Werner, S. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2455 · Cited by 956
Mechanics, malignancy, and metastasis: The force journey of a tumor cell
Kumar, S., & Weaver, V. M. (2009) · Cancer and Metastasis Reviews · doi:10.1007/s10555-008-9173-4 · Cited by 928
Tissue repair and the dynamics of the extracellular matrix
Midwood, K. S., Williams, L. V., & Schwarzbauer, J. E. (2004) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2003.12.003 · Cited by 919
Dynamic interplay between the collagen scaffold and tumor evolution
Egeblad, M., Rasch, M. G., & Weaver, V. M. (2010) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2010.08.015 · Cited by 896
Mechanisms of Mechanotransduction
Orr, A. W., Helmke, B. P., Blackman, B. R., & Schwartz, M. A. (2006) · Developmental Cell · doi:10.1016/j.devcel.2005.12.006 · Cited by 798
Procollagen trafficking, processing and fibrillogenesis
Canty, E. G., & Kadler, K. E. (2005) · Journal of Cell Science · doi:10.1242/jcs.01731 · Cited by 764
Collagen fibrillogenesis: fibronectin, integrins, and minor collagens as organizers and nucleators
Kadler, K. E., Hill, A., & Canty-Laird, E. G. (2008) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2008.06.008 · Cited by 756
Measurement of mechanical tractions exerted by cells in three-dimensional matrices
Legant, W. R., Miller, J. S., Blakely, B. L., Cohen, D. M., Genin, G. M., & Chen, C. S. (2010) · Nature Methods · doi:10.1038/nmeth.1531 · Cited by 647
Sottile, J., & Hocking, D. C. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-01-0048 · Cited by 645
Zhong, C., Chrzanowska-Wodnicka, M., Brown, J., Shaub, A., Belkin, A. M., & Burridge, K. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.2.539 · Cited by 605
Hynes, R. O. (1990) · Springer Series in Molecular Biology · doi:10.1007/978-1-4612-3264-3 · Cited by 537
Schedin, P., & Keely, P. J. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a003228 · Cited by 524
Mechanisms of tissue repair: from wound healing to fibrosis
Mutsaers, S. E., Bishop, J. E., McGrouther, G., & Laurent, G. J. (1997) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/s1357-2725(96)00115-x · Cited by 454
MECHANOTRANSDUCTION INVOLVING MULTIMODULAR PROTEINS: Converting Force into Biochemical Signals
Vogel, V. (2006) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.35.040405.102013 · Cited by 446
Self-citationForce-Induced Unfolding of Fibronectin in the Extracellular Matrix of Living Cells
Smith, M. L., Gourdon, D., Little, W. C., Kubow, K. E., Eguiluz, R. A., Luna-Morris, S., & Vogel, V. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050268 · Cited by 440
Self-citationFibronectin extension and unfolding within cell matrix fibrils controlled by cytoskeletal tension
Baneyx, G., Baugh, L., & Vogel, V. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.072650799 · Cited by 374
Self-citationDallas, S. L., Sivakumar, P., Jones, C. J. P., Chen, Q., Peters, D. M., Mosher, D. F., Humphries, M. J., & Kielty, C. M. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m410762200 · Cited by 316
McDonald, J. A., Kelley, D. G., & Broekelmann, T. J. (1982) · The Journal of cell biology · doi:10.1083/jcb.92.2.485 · Cited by 314
Brightman, A. O., Rajwa, B. P., Sturgis, J. E., McCallister, M. E., Robinson, J. P., & Voytik-Harbin, S. L. (2000) · Biopolymers · doi:10.1002/1097-0282(200009)54:3<222::aid-bip80>3.0.co;2-k · Cited by 292
Fibronectins in vascular morphogenesis
Astrof, S., & Hynes, R. O. (2009) · Angiogenesis · doi:10.1007/s10456-009-9136-6 · Cited by 271
Engineering biomaterials to integrate and heal: The biocompatibility paradigm shifts
Bryers, J. D., Giachelli, C. M., & Ratner, B. D. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24559 · Cited by 271
Klotzsch, E., Smith, M. L., Kubow, K. E., Muntwyler, S., Little, W. C., Beyeler, F., Gourdon, D., Nelson, B. J., & Vogel, V. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0907518106 · Cited by 265
Self-citationRigidity Sensing at the Leading Edge through αvβ3 Integrins and RPTPα
Jiang, G., Huang, A. H., Cai, Y., Tanase, M., & Sheetz, M. P. (2006) · Biophysical Journal · doi:10.1529/biophysj.105.072462 · Cited by 234
A Comparison of the Mechanical and Structural Properties of Fibrin Fibers with Other Protein Fibers
Guthold, M., Liu, W., Sparks, E. A., Jawerth, L. M., Peng, L., Falvo, M., Superfine, R., Hantgan, R. R., & Lord, S. T. (2007) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-007-9001-4 · Cited by 226
Ohashi, T., Kiehart, D. P., & Erickson, H. P. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.5.2153 · Cited by 222
Micro-well arrays for 3D shape control and high resolution analysis of single cells
Ochsner, M., Dusseiller, M. R., Grandin, H. M., Luna-Morris, S., Textor, M., Vogel, V., & Smith, M. L. (2007) · Lab on a Chip · doi:10.1039/b704449f · Cited by 222
Self-citationLocalization of the binding site for cell attachment in the alpha1(I) chain of collagen
Kleinman, H. K., McGoodwin, E. B., Martin, G. R., Klebe, R. J., Fietzek, P. P., & Woolley, D. E. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30315-0 · Cited by 194
Fibronectin-dependent collagen I deposition modulates the cell response to fibronectin
Sottile, J., Shi, F., Rublyevska, I., Chiang, H.-Y., Lust, J., & Chandler, J. (2007) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00130.2007 · Cited by 191
Seong, J., Tajik, A., Sun, J., Guan, J.-L., Humphries, M. J., Craig, S. E., Shekaran, A., García, A. J., Lu, S., Lin, M. Z., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1307405110 · Cited by 190
The removal of extracellular fibronectin from areas of cell-substrate contact
Avnur, Z., & Geiger, B. (1981) · Cell · doi:10.1016/0092-8674(81)90236-1 · Cited by 189
Baneyx, G., Baugh, L., & Vogel, V. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.251422998 · Cited by 188
Self-citationLi, S., Van Den Diepstraten, C., D'souza, S. J., Chan, B. M. C., & Pickering, J. G. (2003) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63464-5 · Cited by 166
Physico-mechanical aspects of extracellular matrix influences on tumorigenic behaviors
Cukierman, E., & Bassi, D. (2010) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2010.04.004 · Cited by 146
Baugh, L., & Viola Vogel, B. (2004) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30026 · Cited by 142
Fibronectin binding site in type I collagen regulates fibronectin fibril formation.
Dzamba, B., Wu, H., Jaenisch, R., & Peters, D. M. P. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.121.5.1165 · Cited by 139
Fibronectin Rigidity Response through Fyn and p130Cas Recruitment to the Leading Edge
Kostić, A., & Sheetz, M. P. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-12-1161 · Cited by 119
Hahn, L. H., & Yamada, K. M. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.3.1160 · Cited by 117
Little, W. C., Smith, M. L., Ebneter, U., & Viola Vogel, B. (2008) · Matrix Biology · doi:10.1016/j.matbio.2008.02.003 · Cited by 114
Self-citationInsidious Changes in Stromal Matrix Fuel Cancer Progression
Miles, F. L., & Sikes, R. A. (2014) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-13-0535 · Cited by 114
Collagen Synthesis in Fibroblasts transformed by Oncogenic Viruses
Green, H., Todaro, G. J., & Goldberg, B. D. (1966) · Nature · doi:10.1038/209916a0 · Cited by 107
Molecular architecture of native fibronectin fibrils
Früh, S. M., Schoen, I., Ries, J., & Viola Vogel, B. (2015) · Nature Communications · doi:10.1038/ncomms8275 · Cited by 107
Antia, M., Baneyx, G., Kubow, K. E., & Viola Vogel, B. (2008) · Faraday Discussions · doi:10.1039/b718714a · Cited by 104
Self-citationChabria, M., Hertig, S., Smith, M. L., & Viola Vogel, B. (2010) · Nature Communications · doi:10.1038/ncomms1135 · Cited by 99
Self-citationErat, M. C., Slatter, D. A., Lowe, E. D., Millard, C. J., Farndale, R. W., Campbell, I. D., & Vakonakis, I. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0812516106 · Cited by 87
Legant, W. R., Chen, C. S., & Viola Vogel, B. (2012) · Integrative Biology · doi:10.1039/c2ib20059g · Cited by 87
Regulation of Tissue Fibrosis by the Biomechanical Environment
Carver, W. E., & Goldsmith, E. C. (2013) · BioMed Research International · doi:10.1155/2013/101979 · Cited by 83
Adipose progenitor cells increase fibronectin matrix strain and unfolding in breast tumors
Chandler, E. M., Saunders, M. P., Yoon, C. J., Gourdon, D., & Fischbach, C. (2011) · Physical Biology · doi:10.1088/1478-3975/8/1/015008 · Cited by 79
Self-citationHocking, D. C., Titus, P. A., Sumagin, R., & Sarelius, I. H. (2007) · Circulation Research · doi:10.1161/circresaha.107.158501 · Cited by 75
A Fibronectin-Independent Mechanism of Collagen Fibrillogenesis in Adult Liver Remodeling
Moriya, K., Bae, E., Honda, K., Sakai, K., Sakaguchi, T., Tsujimoto, I., Kamisoyama, H., Keene, D. R., Sasaki, T., & Sakai, T. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.02.005 · Cited by 71
Kubow, K. E., Klotzsch, E., Smith, M. L., Gourdon, D., Little, W. C., & Viola Vogel, B. (2009) · Integrative Biology · doi:10.1039/b914996a · Cited by 68
Self-citationZhang, Y., Lin, Z., Foolen, J., Schoen, I., Santoro, A., Zenobi‐Wong, M., & Viola Vogel, B. (2014) · Matrix Biology · doi:10.1016/j.matbio.2014.09.001 · Cited by 48
Little, W. C., Schwartländer, R., Smith, M. L., Gourdon, D., & Viola Vogel, B. (2009) · Nano Letters · doi:10.1021/nl902365z · Cited by 45
Self-citationImplications for Collagen Binding from the Crystallographic Structure of Fibronectin 6FnI1–2FnII7FnI
Erat, M. C., Schwarz‐Linek, U., Pickford, A. R., Farndale, R. W., Campbell, I. D., & Vakonakis, I. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.139394 · Cited by 35
Heparin-dependent regulation of fibronectin matrix conformation
Hubbard, B., Buczek‐Thomas, J. A., Nugent, M. A., & Smith, M. L. (2013) · Matrix Biology · doi:10.1016/j.matbio.2013.10.006 · Cited by 33
Self-citationHertig, S., Chabria, M., & Viola Vogel, B. (2012) · Nano Letters · doi:10.1021/nl302153h · Cited by 28
Mitsi, M., Handschin, S., Gerber, I., Schwartländer, R., Klotzsch, E., Wepf, R. A., & Viola Vogel, B. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.08.012 · Cited by 19
Self-citation
Cited by
Nia, H. T., Munn, L. L., & Jain, R. K. (2020) · Science · doi:10.1126/science.aaz0868 · Cited by 855
The role of collagen in cancer: from bench to bedside
Xu, S., Xu, H., Wang, W., Li, S., Li, H., Li, T., Zhang, W., Yu, X., & Liu, L. (2019) · Journal of Translational Medicine · doi:10.1186/s12967-019-2058-1 · Cited by 735
Loebel, C., Mauck, R. L., & Burdick, J. A. (2019) · Nature Materials · doi:10.1038/s41563-019-0307-6 · Cited by 402
Fibronectin: Molecular Structure, Fibrillar Structure and Mechanochemical Signaling
Dalton, C. J., & Lemmon, C. A. (2021) · Cells · doi:10.3390/cells10092443 · Cited by 319
Nanoparticle protein corona: from structure and function to therapeutic targeting
Bashiri, G., Padilla, M. S., Swingle, K. L., Shepherd, S. J., Mitchell, M. J., & Wang, K. (2023) · Lab on a Chip · doi:10.1039/d2lc00799a · Cited by 296
Metadata
- Authors
- Kubow, K. E., Vukmirovic, R., Zhe, L., Klotzsch, E., Smith, M. L., Gourdon, D., Luna, S., & Vogel, V.
- Venue
- Nature Communications
- Published
- 2015-08-14
- Volume
- 6
- Issue
- 1
- Pages
- 8026-8026
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2263006505
- Cited by
- 344
- References
- 62
- FWCI
- 12.33
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
