Cited by 297Nanoparticle-Based Drug DeliveryProtein Interaction Studies and Fluorescence AnalysisProteins in Food Systems

Nanoparticle protein corona: from structure and function to therapeutic targeting

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Bashiri, Ghazal, Padilla, Marshall S., Swingle, Kelsey L., Shepherd, Sarah J., Mitchell, Michael J., Wang, Karin·2023·Lab on a Chip

Abstract

. Since the function of a protein is closely connected to its folded structure, slight differences in the surrounding environment as well as the surface characteristics of the NP materials may cause proteins to lose or gain a function. As a result, this can alter the downstream functionality of the NPs. This review introduces the main biological factors affecting the conformation of proteins associated with the PC. Then, four types of NPs with extensive utility in biomedical applications are described in greater detail, focusing on the conformation and orientation of adsorbed proteins. This is followed by a discussion on the instances in which the conformation of adsorbed proteins can be leveraged for therapeutic purposes, such as controlling protein conformation in assembled matrices in tissue, as well as controlling the PC conformation for modulating immune responses. The review concludes with a perspective on the remaining challenges and unexplored areas at the interface of PC and NP research.

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Authors
Bashiri, G., Padilla, M. S., Swingle, K. L., Shepherd, S. J., Mitchell, M. J., & Wang, K.
Affiliations
  • Temple University (US)
  • University of Pennsylvania (US)
  • Abramson Cancer Center (US)
Corresponding author
Wang, Karin
Venue
Lab on a Chip
Published
2023
Volume
23
Issue
6
Pages
1432-1466
ISSN
  • 1473-0189 (ISSN-L)
  • 1473-0197
OpenAlex
W4317434639
Cited by
296
References
266
Citations by year
2026: 108 · 2025: 103 · 2024: 71 · 2023: 14
FWCI
5.21
Type
journal article
Language
en
Open access
Locations
  • Lab on a Chip (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (CBET-2145491, 1845298)
  • NIH Office of the Director (DP2 TR002776)
  • National Institutes of Health (NCI R01 CA241661, NCI R37 CA244911, NIDDK R01 DK123049)
  • Burroughs Wellcome Fund (Career Award at the Scientific Interface)
  • National Institute of Dental and Craniofacial Research (T90DE030854)
  • Temple University (Startup Funds, Teaching Assistantship)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
protein corona, protein adsorption, protein conformation, protein orientation, nanoparticle surface properties, therapeutic targeting, immunomodulation, biomedical nanoparticles
MeSH terms
Protein Corona, Biological Factors, Protein Conformation, Proteins, Nanoparticles

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