The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
The Notch and Wnt pathways regulate stemness and differentiation in human fallopian tube organoids
Open accessAbstract
The epithelial lining of the fallopian tube is of critical importance for human reproduction and has been implicated as a site of origin of high-grade serous ovarian cancer. Here we report on the establishment of long-term, stable 3D organoid cultures from human fallopian tubes, indicative of the presence of adult stem cells. We show that single epithelial stem cells in vitro can give rise to differentiated organoids containing ciliated and secretory cells. Continuous growth and differentiation of organoids depend on both Wnt and Notch paracrine signalling. Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. Thus, our organoid model provides a much-needed basis for future investigations of signalling routes involved in health and disease of the fallopian tube.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625
Integrated genomic analyses of ovarian carcinoma
Perou, C. M. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/hvp3-wg08 · Cited by 7,925
Identification of stem cells in small intestine and colon by marker gene Lgr5
Barker, N., van Es, J. H., Kuipers, J., Kujala, P., van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P. J., et al. (2007) · Nature · doi:10.1038/nature06196 · Cited by 5,687
Sato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · Nephron · doi:10.1159/000433566 · Cited by 3,515
GOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene lists
Eden, E., Navon, R., Steinfeld, I., Lipson, D., & Yakhini, Z. (2009) · BMC Bioinformatics · doi:10.1186/1471-2105-10-48 · Cited by 3,356
Wnt proteins are lipid-modified and can act as stem cell growth factors
Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L., Reya, T., Yates, J. R., & Nusse, R. (2003) · Nature · doi:10.1038/nature01611 · Cited by 2,257
van Es, J. H., van Gijn, M. E., Riccio, O., van den Born, M., Vooijs, M., Begthel, H., Cozijnsen, M., Robine, S., Winton, D. J., Radtke, F., et al. (2005) · Nature · doi:10.1038/nature03659 · Cited by 1,543
Rethinking ovarian cancer: recommendations for improving outcomes
Vaughan, S., Coward, J. I., Bast, R. C., Berchuck, A., Berek, J. S., Brenton, J. D., Coukos, G., Crum, C. C., Drapkin, R., Etemadmoghadam, D., et al. (2011) · Nature Reviews Cancer · doi:10.1038/nrc3144 · Cited by 1,464
Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling
de Lau, W., Barker, N., Low, T. Y., Koo, B.-K., Li, V. S. W., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P. J., van de Wetering, M., et al. (2011) · Nature · doi:10.1038/nature10337 · Cited by 1,272
LDL-receptor-related proteins in Wnt signal transduction
Tamai, K., Semenov, M., Kato, Y., Spokony, R., Liu, C., Katsuyama, Y., Hess, F., Saint-Jeannet, J.-P., & He, X. (2000) · Nature · doi:10.1038/35035117 · Cited by 1,128
Fundamentals of experimental design for cDNA microarrays
Churchill, G. A. (2002) · Nature Genetics · doi:10.1038/ng1031 · Cited by 1,050
Carmon, K. S., Gong, X., Lin, Q., Thomas, A., & Liu, Q. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1106083108 · Cited by 855
The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent ‘+4’ cell markers
Muñoz, J., Stange, D. E., Schepers, A. G., van de Wetering, M., Koo, B., Itzkovitz, S., Volckmann, R., Kung, K. S., Koster, J., Radulescu, S., et al. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.166 · Cited by 772
Lgr6 Marks Stem Cells in the Hair Follicle That Generate All Cell Lineages of the Skin
Snippert, H. J., Haegebarth, A., Kasper, M., Jaks, V., van Es, J. H., Barker, N., van de Wetering, M., van den Born, M., Begthel, H., Vries, R. G., et al. (2010) · Science · doi:10.1126/science.1184733 · Cited by 751
Isolation and in vitro expansion of human colonic stem cells
Jung, P., Sato, T., Merlos-Suárez, A., Barriga, F. M., Iglesias, M., Rossell, D., Auer, H., Gallardo, M., Blasco, M. A., Sancho, E., et al. (2011) · Nature Medicine · doi:10.1038/nm.2470 · Cited by 709
Discovering motifs in ranked lists of DNA sequences
Eden, E., Lipson, D., Yogev, S., & Yakhini, Z. (2007) · PLoS Computational Biology · doi:10.1371/journal.pcbi.0030039 · Cited by 696
The R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength
de Lau, W., Peng, W. C., Gros, P., & Clevers, H. (2014) · Genes & Development · doi:10.1101/gad.235473.113 · Cited by 656
Redundant sources of Wnt regulate intestinal stem cells and promote formation of Paneth cells
Farin, H. F., Van Es, J. H., & Clevers, H. (2012) · Gastroenterology · doi:10.1053/j.gastro.2012.08.031 · Cited by 655
InParanoid 7: new algorithms and tools for eukaryotic orthology analysis
Ostlund, G., Schmitt, T., Forslund, K., Kostler, T., Messina, D. N., Roopra, S., Frings, O., & Sonnhammer, E. L. L. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkp931 · Cited by 623
Perets, R., Wyant, G. A., Muto, K. W., Bijron, J. G., Poole, B. B., Chin, K. T., Chen, J. Y. H., Ohman, A. W., Stepule, C. D., Kwak, S., et al. (2013) · Cancer Cell · doi:10.1016/j.ccr.2013.10.013 · Cited by 596
Oshimori, N., & Fuchs, E. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2011.11.005 · Cited by 396
Switching on cilia: transcriptional networks regulating ciliogenesis
Choksi, S. P., Lauter, G., Swoboda, P., & Roy, S. (2014) · Development · doi:10.1242/dev.074666 · Cited by 358
Notch and Wnt signals cooperatively control cell proliferation and tumorigenesis in the intestine
Fre, S., Pallavi, S. K., Huyghe, M., Laé, M., Janssen, K.-P., Robine, S., Artavanis-Tsakonas, S., & Louvard, D. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900427106 · Cited by 345
Persistent Sonic Hedgehog Signaling in Adult Brain Determines Neural Stem Cell Positional Identity
Ihrie, R. A., Shah, J. K., Harwell, C. C., Levine, J. H., Guinto, C. D., Lezameta, M., Kriegstein, A. R., & Alvarez-Buylla, A. (2011) · Neuron · doi:10.1016/j.neuron.2011.05.018 · Cited by 255
Chen, Y., Li, H., Huang, C., Twu, N., Yen, M., Wang, P., Chou, T., Liu, Y., Chao, K., & Yang, M. (2010) · The Journal of Pathology · doi:10.1002/path.2761 · Cited by 245
Lgr5 marks stem/progenitor cells in ovary and tubal epithelia
Ng, A., Tan, S., Singh, G., Rizk, P., Swathi, Y., Tan, T. Z., Huang, R. Y.-J., Leushacke, M., & Barker, N. (2014) · Nature Cell Biology · doi:10.1038/ncb3000 · Cited by 218
Lgr5 intestinal stem cells have high telomerase activity and randomly segregate their chromosomes
Schepers, A. G., Vries, R., van den Born, M., van de Wetering, M., & Clevers, H. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.26 · Cited by 217
Paik, D. Y., Janzen, D. M., Schafenacker, A. M., Velasco, V. S., Shung, M. S., Cheng, D., Huang, J., Witte, O. N., & Memarzadeh, S. (2012) · STEM CELLS · doi:10.1002/stem.1207 · Cited by 193
Zhang, Y., Toh, L., Lau, P., & Wang, X. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.368282 · Cited by 180
Secretory cell outgrowth, PAX2 and serous carcinogenesis in the Fallopian tube
Chen, E. Y., Mehra, K., Mehrad, M., Ning, G., Miron, A., Mutter, G. L., Monte, N., Quade, B. J., McKeon, F. D., Yassin, Y., et al. (2010) · The Journal of Pathology · doi:10.1002/path.2739 · Cited by 161
Takashima, S., Kadowaki, M., Aoyama, K., Koyama, M., Oshima, T., Tomizuka, K., Akashi, K., & Teshima, T. (2011) · The Journal of Experimental Medicine · doi:10.1084/jem.20101559 · Cited by 154
Onoufriadis, A., Shoemark, A., Munye, M. M., James, C. T., Schmidts, M., Patel, M., Rosser, E. M., Bacchelli, C., Beales, P. L., Scambler, P. J., et al. (2013) · Journal of Medical Genetics · doi:10.1136/jmedgenet-2013-101938 · Cited by 94
Kessler, M., Zielecki, J., Thieck, O., Mollenkopf, H., Fotopoulou, C., & Meyer, T. F. (2011) · American Journal Of Pathology · doi:10.1016/j.ajpath.2011.09.015 · Cited by 80
Self-citationThe boom in mini stomachs, brains, breasts, kidneys and more
Willyard, C. (2015) · Nature · doi:10.1038/523520a · Cited by 77
Identification of Quiescent, Stem-Like Cells in the Distal Female Reproductive Tract
Wang, Y., Sacchetti, A., van Dijk, M. R., van der Zee, M., van der Horst, P. H., Joosten, R., Burger, C. W., Grootegoed, J. A., Blok, L. J., & Fodde, R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0040691 · Cited by 71
Crum, C. P., Herfs, M. F., Ning, G., Bijron, J. G., Howitt, B. E., Jimenez, C. A., Hanamornroongruang, S., McKeon, F., & Xian, W. (2013) · The Journal of Pathology · doi:10.1002/path.4263 · Cited by 71
Lgr4 is a key regulator of prostate development and prostate stem cell differentiation
Luo, W., Rodríguez, M., Valdez, J. M., Zhu, X., Tan, K., Li, D., Siwko, S. K., Xin, L., & Liu, M. (2013) · Stem Cells · doi:10.1002/stem.1484 · Cited by 70
EphA4 preserves postnatal and adult neural stem cells in an undifferentiated state in vivo
Khodosevich, K., Watanabe, Y., & Monyer, H. (2011) · Journal of Cell Science · doi:10.1242/jcs.076059 · Cited by 67
In vitro three-dimensional modeling of fallopian tube secretory epithelial cells
Lawrenson, K., Notaridou, M., Lee, N., Benjamin, E. O., Jacobs, I. J., Jones, C., & Gayther, S. A. (2013) · BMC Cell Biology · doi:10.1186/1471-2121-14-43 · Cited by 59
Reptin/Ruvbl2 is a Lrrc6/Seahorse interactor essential for cilia motility
Zhao, L., Yuan, S., Cao, Y., Kallakuri, S., Li, Y., Kishimoto, N., DiBella, L. M., & Sun, Z. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1300968110 · Cited by 59
Balenci, L., Wonders, C. P., Coles, B. L. K., Clarke, L., & van der Kooy, D. (2013) · Stem Cells · doi:10.1002/stem.1470 · Cited by 16
Ki-67 and p53 expression of the fallopian tube mucosa in breast cancer patients with hereditary risk
Anton, A., Schott, S., Kaip, G., Rath, M., Heil, J., Aulmann, S., & Sinn, H. (2013) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-013-3062-y · Cited by 2
Cited by
Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities
Nusse, R., & Clevers, H. (2017) · Cell · doi:10.1016/j.cell.2017.05.016 · Cited by 4,342
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196
Human organoids: model systems for human biology and medicine
Kim, J., Koo, B.-K., & Knoblich, J. A. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0259-3 · Cited by 2,187
Drost, J., & Clevers, H. (2018) · Nature Reviews Cancer · doi:10.1038/s41568-018-0007-6 · Cited by 1,790
Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Metadata
- Authors
- Kessler, M., Hoffmann, K., Brinkmann, V., Thieck, O., Jackisch, S., Toelle, B., Berger, H., Mollenkopf, H.-J., Mangler, M., Sehouli, J., Fotopoulou, C., & Meyer, T. F.
- Venue
- Nature Communications
- Published
- 2015-12-08
- Volume
- 6
- Issue
- 1
- Pages
- 8989-8989
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2271458278
- Cited by
- 459
- References
- 43
- FWCI
- 10.29
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
