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Cited by 442

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Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells

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Eminli, Sarah, Foudi, Adlen, Stadtfeld, Matthias, Maherali, Nimet, Ahfeldt, Tim, Mostoslavsky, Gustavo, Hock, Hanno, Hochedlinger, Konrad·2009·Nature Genetics
DOI 10.1038/ng.428
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Abstract

The reprogramming of somatic cells into induced pluripotent stem (iPS) cells upon overexpression of the transcription factors Oct4, Sox2, Klf4 and cMyc is inefficient. It has been assumed that the somatic differentiation state provides a barrier for efficient reprogramming; however, direct evidence for this notion is lacking. Here, we tested the potential of mouse hematopoietic cells at different stages of differentiation to be reprogrammed into iPS cells. We show that hematopoietic stem and progenitor cells give rise to iPS cells up to 300 times more efficiently than terminally differentiated B and T cells do, yielding reprogramming efficiencies of up to 28%. Our data provide evidence that the differentiation stage of the starting cell has a critical influence on the efficiency of reprogramming into iPS cells. Moreover, we identify hematopoietic progenitors as an attractive cell type for applications of iPS cell technology in research and therapy.

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Research works printed but not linked: 43

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Metadata

Authors
Eminli, S., Foudi, A., Stadtfeld, M., Maherali, N., Ahfeldt, T., Mostoslavsky, G., Hock, H., & Hochedlinger, K.
Venue
Nature Genetics
Publisher
Springer Science and Business Media LLC
Published
2009-08-09
Volume
41
Issue
9
Pages
968-976
ISSN
  • 1061-4036 (ISSN-L)
  • 1546-1718
DOI
10.1038/ng.428
OpenAlex
W2100465572
Cited by
442
References
8
FWCI
21.24
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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