The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women
Open accessAbstract
Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries. Here we describe and validate a fluorescence-activated cell sorting-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells can be expanded for months and can spontaneously generate 35- to 50-μm oocytes, as determined by morphology, gene expression and haploid (1n) status. Injection of the human germline cells, engineered to stably express GFP, into human ovarian cortical biopsies leads to formation of follicles containing GFP-positive oocytes 1–2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess rare mitotically active germ cells that can be propagated in vitro as well as generate oocytes in vitro and in vivo.
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Cites
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Self-citationDerivation of Oocytes from Mouse Embryonic Stem Cells
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Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells
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Self-citationParacrine Actions Of Growth Differentiation Factor-9 in the Mammalian Ovary
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Cited by
When stem cells grow old: phenotypes and mechanisms of stem cell aging
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Premature ovarian insufficiency: an international menopause society white paper
Panay, N., Anderson, R. A., Nappi, R. E., Vincent, A. J., Vujovic, S., Webber, L., & Wolfman, W. (2020) · Climacteric · doi:10.1080/13697137.2020.1804547 · Cited by 297
Aging and the environment affect gamete and embryo potential: can we intervene?
Meldrum, D. R., Casper, R. F., Diez-Juan, A., Simon, C., Domar, A. D., & Frydman, R. (2016) · Fertility and Sterility · doi:10.1016/j.fertnstert.2016.01.013 · Cited by 159
Evolutionary medicine and women’s reproductive health
Trevathan, W. R., & Rosenberg, K. R. (2019) · Integrating Evolutionary Biology into Medical Education · doi:10.1093/oso/9780198814153.003.0004 · Cited by 15
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Metadata
- Authors
- White, Y. A. R., Woods, D. C., Takai, Y., Ishihara, O., Seki, H., & Tilly, J. L.
- Venue
- Nature Medicine
- Published
- 2012-02-26
- Volume
- 18
- Issue
- 3
- Pages
- 413-421
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2170550739
- Cited by
- 719
- References
- 37
- FWCI
- 77.55
- Type
- journal article
- Language
- en
- Open access
- green
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