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Cited by 719

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women

Open access
White, Yvonne A R, Woods, Dori C, Takai, Yasushi, Ishihara, Osamu, Seki, Hiroyuki, Tilly, Jonathan L·2012·Nature Medicine
DOI 10.1038/nm.2669
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Abstract

Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries. Here we describe and validate a fluorescence-activated cell sorting-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells can be expanded for months and can spontaneously generate 35- to 50-μm oocytes, as determined by morphology, gene expression and haploid (1n) status. Injection of the human germline cells, engineered to stably express GFP, into human ovarian cortical biopsies leads to formation of follicles containing GFP-positive oocytes 1–2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess rare mitotically active germ cells that can be propagated in vitro as well as generate oocytes in vitro and in vivo.

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Crossref · 10.1038/nm.2669

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Metadata

Authors
White, Y. A. R., Woods, D. C., Takai, Y., Ishihara, O., Seki, H., & Tilly, J. L.
Venue
Nature Medicine
Publisher
Springer Science and Business Media LLC
Published
2012-02-26
Volume
18
Issue
3
Pages
413-421
ISSN
  • 1078-8956 (ISSN-L)
  • 1546-170X
DOI
10.1038/nm.2669
OpenAlex
W2170550739
Cited by
719
References
37
FWCI
77.55
Type
journal article
Language
en
Open access
green

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.