Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Open accessAbstract
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.
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Current state-of-the-art cardiac tissue models are well suited to measure contractile forces generated by cardiomyocytes, but modeling the cardiac pumping function using engineered cardiac tissues has been challenging.
“While current state-of-the-art cardiac tissue models are well suited to measure contractile forces generated by cardiomyocytes (11–13), modeling the cardiac pumping function using engineered cardiac tissues has been challenging.”
From Engineering a living cardiac pump on a chip using high-precision fabrication (2022) · cites this paper as [11]
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- Authors
- Lind, J. U., Busbee, T. A., Valentine, A. D., Pasqualini, F. S., Yuan, H., Yadid, M., Park, S.-J., Kotikian, A., Nesmith, A. P., Campbell, P. H., Vlassak, J. J., Lewis, J. A., & Parker, K. K.
- Affiliations
- Harvard University (US)
- Corresponding author
- Lewis, Jennifer A.
- Parker, Kevin K.
- ORCID
- Venue
- Nature Materials
- Published
- 2016-10-24
- Volume
- 16
- Issue
- 3
- Pages
- 303-308
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- PMC
- PMC5321777
- OpenAlex
- W2536032352
- Cited by
- 863
- References
- 30
- Citations by year
- 2026: 46 · 2025: 72 · 2024: 72 · 2023: 95 · 2022: 88 · 2021: 101 · 2020: 113 · 2019: 91 · 2018: 116 · 2017: 66 · 2016: 1 · 2014: 1
- FWCI
- 43.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institutes of Health (UH3TR000522)
- National Center for Advancing Translational Sciences
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- organ-on-a-chip, multi-material printing, strain gauges, stem cell-derived cardiomyocytes, drug response
- MeSH terms
- Myocardium, Tissue Array Analysis, Printing, Three-Dimensional
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