The DOI matches its Crossref record; 80 of the 80 references it cites were found in a registry or an index.
Profiling metabolites and peptides in single cells
Open accessAbstract
The intracellular levels and spatial localizations of metabolites and peptides reflect the state of a cell and its relationship to its surrounding environment. Moreover, the amounts and dynamics of metabolites and peptides are indicative of normal or pathological cellular conditions. Here we highlight established and evolving strategies for characterizing the metabolome and peptidome of single cells. Focused studies of the chemical composition of individual cells and functionally defined groups of cells promise to provide a greater understanding of cell fate, function and homeostatic balance. Single-cell bioanalytical microanalysis has also become increasingly valuable for examining cellular heterogeneity, particularly in the fields of neuroscience, stem cell biology and developmental biology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
Freudiger, C. W., Min, W., Saar, B. G., Lu, S., Holtom, G. R., He, C., Tsai, J. C., Kang, J. X., & Xie, X. S. (2008) · Science · doi:10.1126/science.1165758 · Cited by 2,255
Generation of a functional mammary gland from a single stem cell
Shackleton, M., Vaillant, F., Simpson, K. J., Stingl, J., Smyth, G. K., Asselin-Labat, M.-L., Wu, L., Lindeman, G. J., & Visvader, J. E. (2006) · Nature · doi:10.1038/nature04372 · Cited by 2,005
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Livet, J., Weissman, T. A., Kang, H., Draft, R. W., Lu, J., Bennis, R. A., Sanes, J. R., & Lichtman, J. W. (2007) · Nature · doi:10.1038/nature06293 · Cited by 1,876 · cited 1×
“One of the most exciting examples of this is the Brainbow mouse, a strain with a multitude of fluorescent labels that have been genetically encoded and expressed in a stochastic manner at varying concentration levels in different neuron”
Cooks, R. G., Ouyang, Z., Takats, Z., & Wiseman, J. M. (2006) · Science · doi:10.1126/science.1119426 · Cited by 1,474
Bandura, D. R., Baranov, V. I., Ornatsky, O. I., Antonov, A., Kinach, R., Lou, X., Pavlov, S., Vorobiev, S., Dick, J. E., & Tanner, S. D. (2009) · Analytical Chemistry · doi:10.1021/ac901049w · Cited by 1,295
Droplet microfluidic technology for single-cell high-throughput screening
Brouzes, E., Medkova, M., Savenelli, N., Marran, D., Twardowski, M., Hutchison, J. B., Rothberg, J. M., Link, D. R., Perrimon, N., & Samuels, M. L. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903542106 · Cited by 1,069
Nemes, P., & Vertes, A. (2007) · Analytical Chemistry · doi:10.1021/ac071181r · Cited by 817
Self-citationNuclear reprogramming to a pluripotent state by three approaches
Yamanaka, S., & Blau, H. M. (2010) · Nature · doi:10.1038/nature09229 · Cited by 776
Towards single-molecule nanomechanical mass spectrometry
Naik, A. K., Hanay, M. S., Hiebert, W. K., Feng, X. L., & Roukes, M. L. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.152 · Cited by 683
Single cell analysis: the new frontier in ‘omics’
Wang, D., & Bodovitz, S. (2010) · Trends in Biotechnology · doi:10.1016/j.tibtech.2010.03.002 · Cited by 673
Proceedings of the 46th ASMS Conference on Mass Spectrometry and Allied Topics
Bruins, A. P. (1998) · Cited by 642
Mosaic Analysis with Double Markers in Mice
Zong, H., Espinosa, J. S., Su, H. H., Muzumdar, M. D., & Luo, L. (2005) · Cell · doi:10.1016/j.cell.2005.02.012 · Cited by 595
Krutzik, P. O., & Nolan, G. P. (2006) · Nature Methods · doi:10.1038/nmeth872 · Cited by 525
High-Resolution Microcoil 1 H-NMR for Mass-Limited, Nanoliter-Volume Samples
Olson, D. L., Peck, T. L., Webb, A. G., Magin, R. L., & Sweedler, J. V. (1995) · Science · doi:10.1126/science.270.5244.1967 · Cited by 503
Self-citationSingle-Cell Microbiology: Tools, Technologies, and Applications
Brehm-Stecher, B. F., & Johnson, E. A. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.3.538-559.2004 · Cited by 501
Clathrate nanostructures for mass spectrometry
Northen, T. R., Yanes, O., Northen, M. T., Marrinucci, D., Uritboonthai, W., Apon, J., Golledge, S. L., Nordström, A., & Siuzdak, G. (2007) · Nature · doi:10.1038/nature06195 · Cited by 480
Behavioural report of single neuron stimulation in somatosensory cortex
Houweling, A. R., & Brecht, M. (2008) · Nature · doi:10.1038/nature06447 · Cited by 456
In vivo lipidomics using single-cell Raman spectroscopy
Wu, H., Volponi, J. V., Oliver, A. E., Parikh, A. N., Simmons, B. A., & Singh, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009043108 · Cited by 436 · cited 1×
“Individual living microalgal cells have been laser-trapped and analyzed by Raman method”
Whisker movements evoked by stimulation of single pyramidal cells in rat motor cortex
Brecht, M., Schneider, M., Sakmann, B., & Margrie, T. W. (2004) · Nature · doi:10.1038/nature02266 · Cited by 430
Generation of a prostate from a single adult stem cell
Leong, K. G., Wang, B.-E., Johnson, L., & Gao, W.-Q. (2008) · Nature · doi:10.1038/nature07427 · Cited by 421
Microfluidic Devices for the High-Throughput Chemical Analysis of Cells
McClain, M. A., Culbertson, C. T., Jacobson, S. C., Allbritton, N. L., Sims, C. E., & Ramsey, J. M. (2003) · Analytical Chemistry · doi:10.1021/ac0346510 · Cited by 383
Antibody‐Conjugated Nanoparticles for Biomedical Applications
Arruebo, M., Valladares, M., & González-Fernández, Á. (2009) · Journal of Nanomaterials · doi:10.1155/2009/439389 · Cited by 331
Markers and Methods for Cell Sorting of Human Embryonic Stem Cell-Derived Neural Cell Populations
Pruszak, J., Sonntag, K.-C., Aung, M. H., Sanchez-Pernaute, R., & Isacson, O. (2007) · Stem Cells · doi:10.1634/stemcells.2006-0744 · Cited by 320
A unifying concept: the history of cell theory
Mazzarello, P. (1999) · Nature Cell Biology · doi:10.1038/8964 · Cited by 307
Fletcher, J. S., Lockyer, N. P., Vaidyanathan, S., & Vickerman, J. C. (2007) · Analytical Chemistry · doi:10.1021/ac061370u · Cited by 302
Gao, J., Yin, X.-F., & Fang, Z.-L. (2004) · Lab on a Chip · doi:10.1039/b310552k · Cited by 300
Gil-Santos, E., Ramos, D., Martínez, J., Fernández-Regúlez, M., García, R., San Paulo, Á., Calleja, M., & Tamayo, J. (2010) · Nature Nanotechnology · doi:10.1038/nnano.2010.151 · Cited by 283
Shrestha, B., & Vertes, A. (2009) · Analytical Chemistry · doi:10.1021/ac901525g · Cited by 273
Current techniques for single-cell lysis
Brown, R. B., & Audet, J. (2008) · Journal of The Royal Society Interface · doi:10.1098/rsif.2008.0009.focus · Cited by 257
Functional consequences of animal-to-animal variation in circuit parameters
Goaillard, J.-M., Taylor, A. L., Schulz, D. J., & Marder, E. (2009) · Nature Neuroscience · doi:10.1038/nn.2404 · Cited by 256
Mellors, J. S., Jorabchi, K., Smith, L. M., & Ramsey, J. M. (2010) · Analytical Chemistry · doi:10.1021/ac902218y · Cited by 235
Kotecha, N., Flores, N. J., Irish, J. M., Simonds, E. F., Sakai, D. S., Archambeault, S., Diaz-Flores, E., Coram, M., Shannon, K. M., Nolan, G. P., et al. (2008) · Cancer Cell · doi:10.1016/j.ccr.2008.08.014 · Cited by 233
Rinia, H. A., Burger, K. N. J., Bonn, M., & Müller, M. (2008) · Biophysical Journal · doi:10.1529/biophysj.108.137737 · Cited by 231
Multiple Ionization Mass Spectrometry Strategy Used To Reveal the Complexity of Metabolomics
Nordström, A., Want, E., Northen, T., Lehtiö, J., & Siuzdak, G. (2008) · Analytical Chemistry · doi:10.1021/ac701982e · Cited by 211
Metabolome diversity: too few genes, too many metabolites?
Schwab, W. (2003) · Phytochemistry · doi:10.1016/s0031-9422(02)00723-9 · Cited by 201
Lapainis, T., Rubakhin, S. S., & Sweedler, J. V. (2009) · Analytical Chemistry · doi:10.1021/ac900936g · Cited by 192
Self-citationChemical and biological single cell analysis
Schmid, A., Kortmann, H., Dittrich, P. S., & Blank, L. M. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.01.007 · Cited by 191
The Hodgkin-Huxley theory of the action potential
Häusser, M. (2000) · Nature Neuroscience · doi:10.1038/81426 · Cited by 187
Bouschen, W., Schulz, O., Eikel, D., & Spengler, B. (2010) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.4401 · Cited by 186
Mizuno, H., Tsuyama, N., Harada, T., & Masujima, T. (2008) · Journal of Mass Spectrometry · doi:10.1002/jms.1460 · Cited by 173
Brain and Visual Perception: The Story of a 25-Year Collaboration
Hubel, D. H., & Wiesel, T. N. (2004) · Cited by 159
Analytical techniques for single-cell metabolomics: state of the art and trends
Amantonico, A., Urban, P. L., & Zenobi, R. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-3850-1 · Cited by 158
Single-neuron labeling with inducible Cre-mediated knockout in transgenic mice
Young, P., Qiu, L., Wang, D., Zhao, S., Gross, J., & Feng, G. (2008) · Nature Neuroscience · doi:10.1038/nn.2118 · Cited by 152
Amantonico, A., Urban, P. L., Fagerer, S. R., Balabin, R. M., & Zenobi, R. (2010) · Analytical Chemistry · doi:10.1021/ac1015326 · Cited by 144
Tyagi, R. K., Azrad, A., Degani, H., et al. (1996) · Magnetic Resonance in Medicine · doi:10.1002/mrm.1910350210 · Cited by 141
Brain and visual perception: The story of a 25‐year collaboration
Davis, J. L. (2006) · Color Research & Application · doi:10.1002/col.20195 · Cited by 139
Measuring the peptides in individual organelles with mass spectrometry
Rubakhin, S. S., Garden, R. W., Fuller, R. R., & Sweedler, J. V. (2000) · Nature Biotechnology · doi:10.1038/72622 · Cited by 137
Self-citationHofstadler, S. A., Severs, J. C., Smith, R., Swanek, F. D., & Ewing, A. G. (1996) · Rapid Communications in Mass Spectrometry · Cited by 131
Vitamin E Imaging and Localization in the Neuronal Membrane
Monroe, E. B., Jurchen, J. C., Lee, J., Rubakhin, S. S., & Sweedler, J. V. (2005) · Journal of the American Chemical Society · doi:10.1021/ja051223y · Cited by 129
Self-citationReview: Microfluidic applications in metabolomics and metabolic profiling
Kraly, J. R., Holcomb, R. E., Guan, Q., & Henry, C. S. (2009) · Analytica Chimica Acta · doi:10.1016/j.aca.2009.08.037 · Cited by 126
Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platform
Cheng, W., Klauke, N., Sedgwick, H., Smith, G. L., & Cooper, J. M. (2006) · Lab on a Chip · doi:10.1039/b608202e · Cited by 126
Ultra-small-sample molecular structure detection using microslot waveguide nuclear spin resonance
Maguire, Y. G., Chuang, I. L., Zhang, S., & Gershenfeld, N. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703001104 · Cited by 110
Chemical Cytometry: Fluorescence-Based Single-Cell Analysis
Cohen, D., Dickerson, J. A., Whitmore, C. D., Turner, E. H., Palcic, M. M., Hindsgaul, O., & Dovic̀hi, N. J. (2008) · Annual Review of Analytical Chemistry · doi:10.1146/annurev.anchem.1.031207.113104 · Cited by 109
Quantitative Measurements of Cell−Cell Signaling Peptides with Single-Cell MALDI MS
Rubakhin, S. S., & Sweedler, J. V. (2008) · Analytical Chemistry · doi:10.1021/ac8010389 · Cited by 99
Self-citationSingle Neuron Analysis by Capillary Electrophoresis with Fluorescence Spectroscopy
Fuller, R. R., Moroz, L. L., Gillette, R., & Sweedler, J. V. (1998) · Neuron · doi:10.1016/s0896-6273(00)80446-8 · Cited by 98
Self-citationKurczy, M. E., Piehowski, P., Bell, C. T. V., Heien, M. L., Winograd, N., & Ewing, A. G. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908101107 · Cited by 84
Induction of pluripotency by defined factors
Okita, K., & Yamanaka, S. (2010) · Experimental Cell Research · doi:10.1016/j.yexcr.2010.04.023 · Cited by 82
Characterizing peptides in individual mammalian cells using mass spectrometry
Rubakhin, S. S., & Sweedler, J. V. (2007) · Nature Protocols · doi:10.1038/nprot.2007.277 · Cited by 80
Self-citationKruse, R. A., Rubakhin, S. S., Romanova, E. V., Bohn, P. W., & Sweedler, J. V. (2001) · Journal of Mass Spectrometry · doi:10.1002/jms.237 · Cited by 77
Self-citationSingle-cell analyses of nitrergic neurons in simple nervous systems
Moroz, L. L., Gillette, R., & Sweedler, J. V. (1999) · Journal of Experimental Biology · doi:10.1242/jeb.202.4.333 · Cited by 73
Self-citationMass spectrometric imaging of peptide release from neuronal cells within microfluidic devices
Jo, K., Heien, M. L., Thompson, L. B., Zhong, M., Nuzzo, R. G., & Sweedler, J. V. (2007) · Lab on a Chip · doi:10.1039/b706940e · Cited by 67
Self-citationRecent advances in the analysis of biological particles by capillary electrophoresis
Košťál, V., & Arriaga, E. A. (2008) · Electrophoresis · doi:10.1002/elps.200700917 · Cited by 62
Cellular Magnetic Resonance Imaging: In Vivo Imaging of Melanoma Cells in Lymph Nodes of Mice
Foster, P. J., Dunn, E. A., Karl, K. E., Snir, J. A., Nycz, C. M., Harvey, A., & Pettis, R. J. (2008) · Neoplasia · doi:10.1593/neo.07937 · Cited by 60
Neuropeptidomics of the Supraoptic Rat Nucleus
Bora, A., Annangudi, S. P., Millet, L. J., Rubakhin, S. S., Forbes, A. J., Kelleher, N. L., Gillette, M. U., & Sweedler, J. V. (2008) · Journal of Proteome Research · doi:10.1021/pr800394e · Cited by 59
Self-citationHsieh, S., Dreisewerd, K., van der Schors, R. C., Jiménez, C. R., Stahl-Zeng, J., Hillenkamp, F., Jorgenson, J. W., Geraerts, W. P. M., & Li, K. W. (1998) · Analytical Chemistry · doi:10.1021/ac9708295 · Cited by 57
A Mass Spectrometry Primer for Mass Spectrometry Imaging
Rubakhin, S. S., & Sweedler, J. V. (2010) · Methods in molecular biology · doi:10.1007/978-1-60761-746-4_2 · Cited by 52
Self-citationMonitoring Real-Time Metabolism of Living Cells by Fast Two-Dimensional NMR Spectroscopy
Motta, A., Paris, D., & Melck, D. J. (2010) · Analytical Chemistry · doi:10.1021/ac9026934 · Cited by 52
Ye, X., Rubakhin, S. S., & Sweedler, J. V. (2007) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2007.10.026 · Cited by 49
Self-citationRecognition of biomarkers and cell‐specific molecular signatures: Aptamers as capture agents
Nery, A. A., Wrenger, C., & Ulrich, H. (2009) · Journal of Separation Science · doi:10.1002/jssc.200800695 · Cited by 47
MR and Iron Magnetic Nanoparticles. Imaging Opportunities in Preclinical and Translational Research
Neumaier, C. E., Baio, G., Ferrini, S., Corte, G., & Daga, A. (2008) · Tumori Journal · doi:10.1177/030089160809400215 · Cited by 46
Reckel, S., Hänsel, R., Löhr, F., & Dötsch, V. (2007) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/j.pnmrs.2007.02.002 · Cited by 44
Winkler, J., Sotiriadou, I., Chen, S., Hescheler, J., & Sachinidis, A. (2009) · Current Medicinal Chemistry · doi:10.2174/092986709789909657 · Cited by 40
Subcellular In Vivo 1H MR Spectroscopy of Xenopus laevis Oocytes
Lee, S., Cho, J., Mietchen, D., Kim, Y., Hong, K. S., Lee, C., Kang, D., Park, K. D., Choi, B., & Cheong, C. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.073502 · Cited by 36
Miao, H., Rubakhin, S. S., Scanlan, C., Wang, L., & Sweedler, J. V. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03791.x · Cited by 35
Self-citationAnterograde Axonal Tract Tracing
Raju, D. V., & Smith, Y. (2006) · Current Protocols in Neuroscience · doi:10.1002/0471142301.ns0114s37 · Cited by 29
Gene transfer in the nervous system and implications for transsynaptic neuronal tracing
Huh, Y., Oh, M. S., Leblanc, P. R., & Kim, K. (2010) · Expert Opinion on Biological Therapy · doi:10.1517/14712591003796538 · Cited by 27
Extra- and intracellular metabolite concentrations for murine hybridoma cells
Schmid, G., & Blanch, H. W. (1992) · Applied Microbiology and Biotechnology · doi:10.1007/bf00183239 · Cited by 22
Conte-Perales, L., Barroso‐Chinea, P., Rico, A. J., Gómez-Bautista, V., López, I. P., Roda, E., Wouterlood, F. G., & Lanciego, J. L. (2009) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2009.11.021 · Cited by 10
Cited by
Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices
Halldorsson, S., Lucumi, E., Gómez-Sjöberg, R., & Fleming, R. M. T. (2015) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.07.029 · Cited by 1,027
Liu, X., & Locasale, J. W. (2017) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2017.01.004 · Cited by 453
Single-cell proteomics enabled by next-generation sequencing or mass spectrometry
Bennett, H. M., Stephenson, W., Rose, C. M., & Darmanis, S. (2023) · Nature Methods · doi:10.1038/s41592-023-01791-5 · Cited by 351
Lombard‐Banek, C., Moody, S. A., & Nemes, P. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201510411 · Cited by 214
Self-citationZhu, H., Zou, G., Wang, N., Zhuang, M., Xiong, W., & Huang, G. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1615557114 · Cited by 133
Metadata
- Authors
- Rubakhin, S. S., Romanova, E. V., Nemes, P., & Sweedler, J. V.
- Venue
- Nature Methods
- Published
- 2011-03-30
- Volume
- 8
- Issue
- S4
- Pages
- S20-S29
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2081412654
- Cited by
- 347
- References
- 80
- FWCI
- 13.64
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
