A public genome-scale lentiviral expression library of human ORFs
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Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
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Current CRISPR sgRNA libraries can be decoded directly via the CROP-seq vector, while other perturbations, such as ORFs or non-coding elements, can be tagged with short barcodes.
“Existing CRISPR sgRNA libraries can be read out directly (using the CROP-seq vector) while other perturbations (e.g., ORFs, non-coding sequences) can be paired with short barcodes (Melnikov et al., 2012; Yang et al., 2011).”
From Optical Pooled Screens in Human Cells (2019) · cites this paper as Yang et al., 2011
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- Authors
- Yang, X., Boehm, J. S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., Lubonja, R., Thomas, S. R., Alkan, O., Bhimdi, T., Green, T. M., Johannessen, C. M., Silver, S. J., Nguyen, C., Murray, R. R., Hieronymus, H., Balcha, D., Fan, C., Lin, C., Ghamsari, L., Vidal, M., Hahn, W. C., Hill, D. E., & Root, D. E.
- Affiliations
- Broad Institute (US)
- Harvard University (US)
- Corresponding author
- Vidal, Marc
- Hahn, William C
- Hill, David E
- Root, David E
- ORCID
- Venue
- Nature Methods
- Published
- 2011-06-26
- Volume
- 8
- Issue
- 8
- Pages
- 659-661
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3234135
- OpenAlex
- W2023994677
- Cited by
- 570
- References
- 20
- Citations by year
- 2026: 35 · 2025: 40 · 2024: 35 · 2023: 49 · 2022: 26 · 2021: 48 · 2020: 52 · 2019: 58 · 2018: 40 · 2017: 35 · 2016: 40 · 2015: 44 · 2014: 31 · 2013: 18 · 2012: 15
- FWCI
- 13.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (P01CA142536, R33CA128625)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- ORFeome, Gateway cloning, loss-of-function screen, gain-of-function screen, functional genomics, mammalian cell expression
- MeSH terms
- Cloning, Molecular, Genetic Vectors, Humans, Genomic Library, Lentivirus, Open Reading Frames
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