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Miniaturized integration of a fluorescence microscope
Open accessAbstract
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including a semiconductor light source and sensor. This device enables high-speed cellular imaging across ∼0.5 mm2 areas in active mice. This capability allowed concurrent tracking of Ca2+ spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca2+ spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.
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Metadata
- Authors
- Ghosh, K. K., Burns, L. D., Cocker, E. D., Nimmerjahn, A., Ziv, Y., Gamal, A. E., & Schnitzer, M. J.
- Venue
- Nature Methods
- Published
- 2011-09-11
- Volume
- 8
- Issue
- 10
- Pages
- 871-878
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2077374589
- Cited by
- 1,120
- References
- 39
- FWCI
- 17.01
- Type
- journal article
- Language
- en
- Open access
- green
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