The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Precise and reliable gene expression via standard transcription and translation initiation elements
Open accessAbstract
An inability to reliably predict quantitative behaviors for novel combinations of genetic elements limits the rational engineering of biological systems. We developed an expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli: transcription elements encode a common mRNA start, and translation elements use an overlapping genetic motif found in many natural systems. We engineered libraries of constitutive and repressor-regulated promoters along with translation initiation elements following these definitions. We measured activity distributions for each library and selected elements that collectively resulted in expression across a 1,000-fold observed dynamic range. We studied all combinations of curated elements, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability. We expect the genetic element definitions validated here can be collectively expanded to create collections of public-domain standard biological parts that support reliable forward engineering of gene expression at genome scales.
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Self-citationCoding-Sequence Determinants of Gene Expression in Escherichia coli
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Meyer, V., Basenko, E. Y., Benz, J. P., Braus, G. H., Caddick, M. X., Csukai, M., de Vries, R. P., Endy, D., Frisvad, J. C., Gunde-Cimerman, N., et al. (2020) · Fungal Biology and Biotechnology · doi:10.1186/s40694-020-00095-z · Cited by 515
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Metadata
- Authors
- Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D.
- Venue
- Nature Methods
- Published
- 2013-03-10
- Volume
- 10
- Issue
- 4
- Pages
- 354-360
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2001426985
- Cited by
- 764
- References
- 8
- FWCI
- 28.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods. https://doi.org/10.1038/nmeth.2404
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
