Cas9 as a versatile tool for engineering biology

Mali, Prashant, Esvelt, Kevin M, Church, George M·2013·Nature Methods

Cited by (999)

Items 401–500 of 999

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  3. CRISPR in Animals and Animal Models
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  4. Generation of albino medaka (Oryzias latipes) by CRISPR/Cas9
    Jian Fang, Tiansheng Chen, Qihua Pan, Qian Wang · 2018 · Journal of Experimental Zoology Part B Molecular and Developmental Evolution
  5. Knock-In Rat Lines with Cre Recombinase at the Dopamine D1 and Adenosine 2a Receptor Loci
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  6. Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression
    Ami M. Kabadi, Charles A. Gersbach · 2014 · Methods
  7. Genome editing strategies: potential tools for eradicating HIV-1/AIDS
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  8. Behavioral sequelae of astrocyte dysfunction: focus on animal models of schizophrenia
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  9. Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system
    Mingming Wu, Caihong Wei, Zhengxing Lian, Ruizao Liu, Caiye Zhu, Huihua Wang, Jiaxue Cao, Yuelei Shen, Fuping Zhao, Li Zhang et al. · 2016 · Scientific Reports
  10. Gene Editing and Genetic Lung Disease. Basic Research Meets Therapeutic Application
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  11. Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions
    Jiao Lin, Yan Liu, Peidong Lai, Huixia Ye, Liang Xu · 2020 · Nucleic Acids Research
  12. P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri
    Yang Wei Huan, Vincenzo Torraca, Russell Brown, Jidapha Fa-arun, Sydney L. Miles, Diego A. Oyarzún, Serge Mostowy, Baojun Wang · 2023 · ACS Synthetic Biology
  13. Efficient production of d-tagatose via DNA scaffold mediated oxidoreductases assembly in vivo from whey powder
    Wei Liu, Zhilin Zhang, Ying Li, Liying Zhu, Ling Jiang · 2023 · Food Research International
  14. Enriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene
    Shuren Liao, Margaret Tammaro, Hong Yan · 2015 · Nucleic Acids Research
  15. CRISPR Epigenome Editing of AKAP150 in DRG Neurons Abolishes Degenerative IVD-Induced Neuronal Activation.
    J. Stover, Niloofar Farhang, Kristofer C. Berrett, J. Gertz, Brandon D. Lawrence, R. Bowles · 2017 · Molecular Therapy
  16. Recent advances in understanding xenotransplantation: implications for the clinic
    David K. C. Cooper, Rita Bottino · 2015 · Expert Review of Clinical Immunology
  17. The Potential of the Combination of CRISPR/Cas9 and Pluripotent Stem Cells to Provide Human Organs from Chimaeric Pigs
    Wanyou Feng, Yifan Dai, Lisha Mou, David K. C. Cooper, Deshun Shi, Zhiming Cai · 2015 · International Journal of Molecular Sciences
  18. Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system
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  19. Exploring targets of TET2-mediated methylation reprogramming as potential discriminators of prostate cancer progression
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  20. CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway
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  21. Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor
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  22. A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana
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  23. Mechanisms regulating the CRISPR-Cas systems
    Marta Zakrzewska, Michał Burmistrz · 2023 · Frontiers in Microbiology
  24. RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
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  25. Epigenetic modulation, stress and plasticity in susceptibility of the snail host, Biomphalaria glabrata, to Schistosoma mansoni infection
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  26. Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells
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  27. Strategies for mitochondrial gene editing
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  28. Genome engineering: Drosophila melanogaster and beyond
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  29. Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes
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  30. CRISPR/Cas9-mediated correction of human genetic disease
    Ke Men, Xingmei Duan, Zhiyao He, Yang Yang, Shaohua Yao, Yuquan Wei · 2017 · Science China Life Sciences
  31. Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9
    Hyung Suk Oh, Werner Neuhausser, Pierce Eggan, Magdalena Angelova, Rory D. Kirchner, Kevin C. Eggan, David M. Knipe · 2019 · eLife
  32. Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
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  33. CrisprGE: a central hub of CRISPR/Cas-based genome editing
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  34. Rapid and efficient CRISPR/Cas9 gene inactivation in human neurons during human pluripotent stem cell differentiation and direct reprogramming
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  35. Disruption of the sheep BMPR-IB gene by CRISPR/Cas9 in in vitro -produced embryos
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  36. Allele-Specific CRISPR/Cas9 Correction of a Heterozygous DNM2 Mutation Rescues Centronuclear Myopathy Cell Phenotypes
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  40. Disruption of long-range gene regulation in human genetic disease: a kaleidoscope of general principles, diverse mechanisms and unique phenotypic consequences
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  41. Genetics and Genomics of Coronary Artery Disease
    Milos Pjanic, Clint L. Miller, Robert C. Wirka, Juyong Brian Kim, Daniel M. DiRenzo, Thomas Quertermous · 2016 · Current Cardiology Reports
  42. CRISPR‐Cas: From the Bacterial Adaptive Immune System to a Versatile Tool for Genome Engineering
    Marion Kirchner, Sabine Schneider · 2015 · Angewandte Chemie International Edition
  43. DNA-based control of protein activity
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  44. The Development of TALE Nucleases for Biotechnology
    David G. Ousterout, Charles A. Gersbach · 2015 · Methods in molecular biology
  45. Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
    Prarthana Mohanraju, John van der OOST, Martin Jínek, Daan C. Swarts · 2018 · BIO-PROTOCOL
  46. Photocontrol of CRISPR/Cas9 function by site-specific chemical modification of guide RNA
    Yang Wang, Yan Liu, Fan Xie, Jiao Lin, Liang Xu · 2020 · Chemical Science
  47. Human iPSCs and Genome Editing Technologies for Precision Cardiovascular Tissue Engineering
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  48. Genome editing techniques in plants: a comprehensive review and future prospects toward zero hunger
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  49. CRISPR Primer Designer: Design primers for knockout and chromosome imaging CRISPR‐Cas system
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  50. Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys
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  51. Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals
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  53. Delivery methods for CRISPR/Cas9 gene editing in crustaceans
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  54. DTR‐mediated conditional cell ablation—Progress and challenges
    Christiane Ruedl, Steffen Jung · 2018 · European Journal of Immunology
  55. On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains
    Gabriela Pannunzio Carmignotto, Adriano Rodrigues Azzoni · 2019 · Journal of Biotechnology
  56. Application of CRISPR/Cas9 System in Establishing Large Animal Models
    Yingqi Lin, Jun Li, Caijuan Li, Zhuchi Tu, Shihua Li, Xiao‐Jiang Li, Sen Yan · 2022 · Frontiers in Cell and Developmental Biology
  57. Targeted genome editing in Aedes aegypti using TALENs
    Azadeh Aryan, Kevin M. Myles, Zach N. Adelman · 2014 · Methods
  58. Dendritic cell specific targeting of MyD88 signalling pathways in vivo
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  59. Stem cell manipulation, gene therapy and the risk of cancer stem cell emergence
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  60. Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression
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  61. Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation
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  62. New Horizons in the Treatment of Corneal Endothelial Dysfunction
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  63. Methods for targeted mutagenesis in zebrafish using TALENs
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  64. Anopheline Reproductive Biology: Impacts on Vectorial Capacity and Potential Avenues for Malaria Control
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  65. Translatable gene therapy for lung cancer using Crispr CAS9—an exploratory review
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  66. Delivery of genome editing tools: A promising strategy for HPV-related cervical malignancy therapy
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  98. Engineering of Isogenic Cells Deficient for MR1 with a CRISPR/Cas9 Lentiviral System: Tools To Study Microbial Antigen Processing and Presentation to Human MR1-Restricted T Cells
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