Cas9 as a versatile tool for engineering biology

Mali, Prashant, Esvelt, Kevin M, Church, George M·2013·Nature Methods

Cited by (999)

Items 801–900 of 999

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  3. A Novel Screening Approach for the Dissection of Cellular Regulatory Networks of NF-κB Using Arrayed CRISPR gRNA Libraries
    Patrick Joseph O'Shea, Jan Wildenhain, Mathew Leveridge, Chetana M. Revankar, Jianping Yang, Jenna Bradley, Mike A. Firth, James E. Pilling, David R. Piper, Jonathan D. Chesnut et al. · 2020 · SLAS DISCOVERY
  4. CRISPR/Cas-Based Insect Resistance in Crops
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  5. Multiple sgRNAs for one-step inactivation of the duplicatedacetyl-coenzyme A carboxylase 2(ACC2) genes inBrassica napus
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  7. The Past, Present, and Future of Genetically Engineered Mouse Models for Skeletal Biology
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  8. Use of theHPRTgene to study nuclease-induced DNA double-strand break repair
    Polly Gravells, Sara Ahrabi, Rajani Kanth Vangala, Kazunori Tomita, James T. Brash, Lena A. Brustle, Christopher P. Chung, Julia M. Hong, Aikaterini Kaloudi, Timothy C. Humphrey et al. · 2015 · Human Molecular Genetics
  9. Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells
    Dan Gao, Fu‐Sen Liang · 2018 · Methods in molecular biology
  10. Advances in CRISPR/Cas9 Technology for in Vivo Translation
    Yağız Anıl Çiçek, David C. Luther, Jessica A. Kretzmann, Vincent M. Rotello · 2019 · Biological and Pharmaceutical Bulletin
  11. Eradication of Human Immunodeficiency Virus Type-1 (HIV-1)-Infected Cells
    Nejat Düzgüneş, Krystyna Konopka · 2019 · Pharmaceutics
  12. Site-specific targeting of a light activated dCas9-KillerRed fusion protein generates transient, localized regions of oxidative DNA damage
    Nealia C. M. House, Ramya Parasuram, Jacob V. Layer, Brendan D. Price · 2020 · PLoS ONE
  13. Editing the Future: CRISPR/Cas9 for Climate-Resilient Crops
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  14. Diagnosis of Infectious Diseases by CRISPR/Cas System
    Mahintaj Dara, Negin Shafieipour, Mahsa Saffar, Mehdi Dianatpour, Seyed-Mohammad-Bagher Tabei, S. M. M. Dastgheib · 2025 · OBM Genetics
  15. A HO-1 gene knockout using a NanoCRISPR scaffold suppresses metastasis in mouse models
    Ning Wang, Zichao Luo, Chao Liu, Jialin Ma, Xinyue Wu, Qinjie Wu, Xiaogang Liu, Changyang Gong · 2025 · Nature Biomedical Engineering
  16. Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity
    Quentin M. Smith, Sylvia Whittle, R. Aramayo, Daniel E. Rollins, Adam S. B. Jalal, Deborah I. Egharevba, Kyle L. Morris, Alice L. B. Pyne, David S. Rueda · 2026 · Nature
  17. Neuroepigenetic Editing
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  18. Phenomics reveals a novel putative chloroplast fatty acid transporter in the marine diatom Skeletonema marinoi involved in temperature acclimation
    Oskar Johansson, Mats Töpel, Jenny Egardt, Matthew I. M. Pinder, Mats X. Andersson, Anna Godhe, Adrian K. Clarke · 2019 · Scientific Reports
  19. A Survey on Shortest Unique Substring Queries
    Paniz Abedin, Muhammed Oğuzhan Külekci, Shama Thankachan · 2020 · Algorithms
  20. Modulation of Insulin Gene Expression with CRISPR/Cas9-based Transcription Factors
    Bakhytzhan Alzhanuly, Zhussipbek Mukhatayev, Dauren M. Botbayev, Yeldar Ashirbekov, Nurlubek Katkenov, Nurlan T. Dzhaynakbaev, Kamalidin O. Sharipov · 2021 · Open Access Macedonian Journal of Medical Sciences
  21. Development of Transformation for Genome Editing of an Emerging Model Organism
    Yutaka Yamamoto, Susan A. Gerbi · 2022 · Genes
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  23. Leveraging synthetic biology for tissue engineering applications
    Esther J. Lee, Jeffrey J. Tabor, Antonios G. Mikos · 2014 · Inflammation and Regeneration
  24. Engineered T cell therapies
    Anne-Christine Field, Waseem Qasim · 2015 · Expert Reviews in Molecular Medicine
  25. CRISPR‐Cas: von einem bakteriellen adaptiven Immunsystem zu einem vielseitigen Werkzeug für die Gentechnik
    Marion Kirchner, Sabine Schneider · 2015 · Angewandte Chemie
  26. OMPPM: online multiple palindrome pattern matching
    Hwee Kim, Yo-Sub Han · 2015 · Bioinformatics
  27. An Enhancer Haplotype may Influence BCL11A Expression Levels and the Response to Hydroxyurea in β-Thalassemia Patients
    Nahal Maroofi, Azita Azarkeivan, Soosan Banihashemi, Saeid Mohammadparast, Ali Aghajanirefah, Mehdi Banan · 2017 · Pharmacogenomics
  28. CRISPR/Cas9 System to Knockdown MicroRNA In Vitro and In Vivo
    Bin Yi, Kristina Larter, Yaguang Xi · 2021 · Methods in molecular biology
  29. CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus
    Raghavendra Yadavalli, Kousuke Umeda, Hannah A. Waugh, Adrienne N. Tracy, Asha V. Sidhu, Derek E. Hernández, José A. Fernández Robledo · 2021 · Frontiers in Bioengineering and Biotechnology
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  31. Genome Engineering for Therapeutic Applications
    Pratiksha I. Thakore, Charles A. Gersbach · 2015 · Elsevier eBooks
  32. Approaches to functionally validate candidate genetic variants involved in colorectal cancer predisposition
    Laia Bonjoch, Pilar Mur, Coral Arnau‐Collell, Gardenia Vargas‐Parra, Bahar Shamloo, Sebastià Franch‐Expósito, Marta Pineda, Gabriel Capellá, Batu Erman, Sergi Castellvı́-Bel · 2019 · Molecular Aspects of Medicine
  33. RNA-Guided CRISPR-Cas9 System for Removal of Microbial Pathogens
    Gargi Bhattacharjee, Khushal Khambhati, Vijai Singh · 2019 · Springer eBooks
  34. Development of a Facile Approach for Generating Chemically Modified CRISPR/Cas9 RNA
    Tristan A. Scott, Citradewi Soemardy, Kevin V. Morris · 2020 · Molecular Therapy — Nucleic Acids
  35. Bioprocess engineering: micromanaging Chinese hamster ovary cell phenotypes
    Paul S. Kelly, Colin Clarke, MARTIN M. CLYNES, Niall Barron · 2014 · Pharmaceutical Bioprocessing
  36. Reworked Discocyclina occurring together with Lepidocyclina in the Oligocene of San Vicente de la Barquera (northern Spain)
    José Serra Kiel, Carles Ferràndez‐Cañadell, Josep Tosquella · 1999 · Revista española de micropaleontología
  37. Genome-wide CRISPR knockout screen reveals membrane tethering complexes EARP and GARP important for Bovine Herpes Virus Type 1 replication
    Wenfang Spring Tan, Enguang Rong, Inga R. Dry, Simon Geoffrey Lillico, Andy S Law, Christopher B.A. Whitelaw, Robert G. Dalziel · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
  38. Therapeutic Strategies of Targeting Gut Microbiota in Colorectal Cancer Management
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  39. Applications of CRISPR-Cas in its natural habitat
    Alexander P. Hynes, Marie-Laurence Lemay, Sylvain Moineau · 2016 · Current Opinion in Chemical Biology
  40. Validation of Candidate Host Cell Entry Factors for Bovine Herpes Virus Type-1 Based on a Genome-Wide CRISPR Knockout Screen
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  41. GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting
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  42. Pathogen-insect interaction candidate molecules for transmission-blocking control strategies of vector borne diseases
    Federico Zumaya-Estrada, María C. Rodríguez, Mario Henry Rodriguez · 2017 · Salud Pública de México
  43. Application and Development of CRISPR/Cas9 Technology in Pig Research
    Huafeng Lin, Qiudi Deng, Lili Li, Lei Shi · 2019 · IntechOpen eBooks
  44. Engineering bone phenotypes in domestic animals: Unique resources for enhancing musculoskeletal research
    Larry J. Suva, Mark E. Westhusin, Charles R. Long, Dana Gaddy · 2019 · Bone
  45. CRISPR-Cas9 expressed in stably transduced cell lines promotes recombination and selects for herpes simplex virus recombinants
    Hyung Suk Oh, Fernando M. Diaz, Changhong Zhou, Nicholas Carpenter, David M. Knipe · 2022 · Current Research in Virological Science
  46. When push comes to shove - RNA polymerase and DNA-bound protein roadblocks
    Nan Hao, Alana J. Donnelly, Ian B. Dodd, Keith Edward Shearwin · 2023 · Biophysical Reviews
  47. Establishment of a genome editing tool using CRISPR-Cas9 ribonucleoprotein complexes in the non-model plant pathogen Sphaerulina musiva
    Joanna Tannous, Cole R. Sawyer, Md Mahmudul Hassan, Jessy Labbé, Carrie A. Eckert · 2023 · Frontiers in Genome Editing
  48. Neocarzilin Inhibits Cancer Cell Proliferation via BST-2 Degradation, Resulting in Lipid Raft-Trapped EGFR
    Josef Braun, Yudong Hu, Adrian T. Jauch, Thomas F. Gronauer, Julia Mergner, Nina C. Bach, Franziska R. Traube, Stefan Zahler A, Stephan A. Sieber · 2024 · JACS Au
  49. Effective Gene Delivery to Valvular Interstitial Cells Using Adeno-Associated Virus Serotypes 2 and 3
    Fergus F. Wong, Michelle L. Ho, Momona Yamagami, Michael T. Lam, Kathryn Jane Grande-Allen, Junghae Suh · 2015 · Tissue Engineering Part C Methods
  50. An integrated miRNA functional screening and target validation method for organ morphogenesis
    Ivan T. Rebustini, Maryann Vlahos, Trevor S. Packer, Maria A. Kukuruzinska, Richard L. Maas · 2016 · Scientific Reports
  51. VPS4B deficiency causes early embryonic lethality and induces signal transduction disorders of cell endocytosis
    Danna Chen, Fei He, Ting Lu, Jin Huang, Meiyi Li, Decheng Cai, Cheng Huang, Dong Chen, Fu Xiong · 2021 · genesis
  52. Monitoring of Dual CRISPR/Cas9-Mediated Steroidogenic Acute Regulatory Protein Gene Deletion and Cholesterol Accumulation Using High-Resolution Fluorescence In Situ Hybridization in a Single Cell
    Jinwoo Lee, Colin R. E. Jefcoate · 2017 · Frontiers in Endocrinology
  53. Chromatin organization in pluripotent cells: emerging approaches to study and disrupt function
    Clara Lopes Novo, Peter J. Rugg‐Gunn · 2015 · Briefings in Functional Genomics
  54. Targeted mutagenesis by an optimized agrobacterium‐delivered CRISPR / Cas 9 system in the model legume Medicago truncatula
    Yingying Meng, Chongnan Wang, Pengcheng Yin, Butuo Zhu, Pengcheng Zhang, Lifang Niu, Hao Lin · 2019 · The Model Legume Medicago truncatula
  55. Sex control strategies in aquaculture: A systematic review of aromatase inhibition
    Mohd Ashraf Malik, Pallath Muhammed Nuzaiba, Irshad Ahmad, T. Paul Nathaniel, Arya Prabhakaran, Subodh Gupta, Tincy Varghese · 2025 · Aquaculture
  56. A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi
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  57. Excision of Integrated Human Herpesvirus 6A Genomes Using CRISPR/Cas9 Technology
    Giulia Aimola, Darren J. Wight, Louis Flamand, Benedikt B. Kaufer · 2023 · Microbiology Spectrum
  58. Predicting the effect of CRISPR-Cas9-based epigenome editing
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  59. Current application and future perspective of CRISPR/cas9 gene editing system mediated immune checkpoint for liver cancer treatment
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  60. Development and optimization of base editors and its application in crops
    Chuandong Jiang, Yangyang Li, Ran Wang, Xiao Sun, Yan Zhang, Qiang Zhang · 2024 · Biochemical and Biophysical Research Communications
  61. Transgene-Free Genome Editing by Germline Injection of CRISPR/Cas RNA
    Hillel T. Schwartz, Paul W. Sternberg · 2014 · Methods in enzymology on CD-ROM/Methods in enzymology
  62. From noise to synthetic nucleoli: can synthetic biology achieve new insights?
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  63. Establishment of IL-7 Expression Reporter Human Cell Lines, and Their Feasibility for High-Throughput Screening of IL-7-Upregulating Chemicals
    Yeon Sook Cho, Byung‐Soo Kim, Chan Kyu Sim, Inki Kim, Myeong Sup Lee · 2016 · PLoS ONE
  64. Aging Genome Modification and Editing using the Crispr-Cas9 system: Anti-Alzheimer Study by Docking Methods
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  65. Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
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  66. Implications of CRISPR-Cas9 Genome Editing Methods in Atherosclerotic Cardiovascular Diseases
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  67. The applications of CRISPR/Cas-mediated genome editing in genetic hearing loss
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  68. New Transformation Technologies for Trees
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  70. Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System
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  71. Neurons under genetic control: What are the next steps towards the treatment of movement disorders?
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  82. Predicting the effect of CRISPR-Cas9-based epigenome editing
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