The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Isolation of single-base genome-edited human iPS cells without antibiotic selection
Open accessAbstract
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease–induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
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Cited by
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CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs
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Self-citationCRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons
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“Alternative strategies for increasing editing efficiencies include methods to more efficiently detect and subclone cells that have undergone rare editing events (Miyaoka et al., 2014) as well as to enhance delivery methods for the nuclease and donor template (Lin et al.”
MethodGenome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis
Tian, R., Abarientos, A., Hong, J., Hashemi, S. H., Yan, R., Dräger, N., Leng, K., Nalls, M. A., Singleton, A. B., Xu, K., et al. (2021) · Nature Neuroscience · doi:10.1038/s41593-021-00862-0 · Cited by 414
Metadata
- Authors
- Miyaoka, Y., Chan, A. H., Judge, L. M., Yoo, J., Huang, M., Nguyen, T. D., Lizarraga, P. P., So, P.-L., & Conklin, B. R.
- Venue
- Nature Methods
- Published
- 2014-02-09
- Volume
- 11
- Issue
- 3
- Pages
- 291-293
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2003418092
- Cited by
- 301
- References
- 30
- FWCI
- 10.10
- Type
- journal article
- Language
- en
- Open access
- green
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