The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Isolation of single-base genome-edited human iPS cells without antibiotic selection
Open accessAbstract
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease–induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
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Cites
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Self-citationGenome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis
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Metadata
- Authors
- Miyaoka, Y., Chan, A. H., Judge, L. M., Yoo, J., Huang, M., Nguyen, T. D., Lizarraga, P. P., So, P.-L., & Conklin, B. R.
- Venue
- Nature Methods
- Published
- 2014-02-09
- Volume
- 11
- Issue
- 3
- Pages
- 291-293
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2003418092
- Cited by
- 301
- References
- 30
- FWCI
- 10.10
- Type
- journal article
- Language
- en
- Open access
- green
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