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Cited by 550RNA and protein synthesis mechanismsRNA modifications and cancerGenomics and Phylogenetic Studies

Efficient and quantitative high-throughput tRNA sequencing

Open access
Zheng, Guanqun, Qin, Yidan, Clark, Wesley C, Dai, Qing, Yi, Chengqi, He, Chuan, Lambowitz, Alan M, Pan, Tao·2015·Nature Methods
DOI 10.1038/nmeth.3478PMC PMC4624326
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Abstract

Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.

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  • High-throughput tRNA-specific sequencing techniques have been developed.

    “More recently, high-throughput tRNA-specific sequencing techniques have also been developed.”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [139]

  • Enzymatic removal of tRNA modifications and thermostable group II intron reverse transcriptase are combined to overcome the blocks caused by modifications and structure.

    “Enzymatic removal of tRNA modifications and thermostable group II intron reverse transcriptase are combined to overcome the blocks caused by modifications and structure (139).”

    From Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication (2019) · cites this paper as [139]

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Research works printed but not linked: 20

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Authors
Zheng, G., Qin, Y., Clark, W. C., Dai, Q., Yi, C., He, C., Lambowitz, A. M., & Pan, T.
Affiliations
  • University of Chicago (US)
  • The University of Texas at Austin (US)
  • Howard Hughes Medical Institute (US)
Corresponding author
  • Lambowitz, Alan M
  • Pan, Tao
ORCID
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2015-07-27
Volume
12
Issue
9
Pages
835-837
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/nmeth.3478
PMC
PMC4624326
OpenAlex
W2114147818
Cited by
550
References
2
Citations by year
2026: 39 · 2025: 53 · 2024: 57 · 2023: 58 · 2022: 53 · 2021: 61 · 2020: 58 · 2019: 58 · 2018: 36 · 2017: 37 · 2016: 35 · 2015: 5
FWCI
16.51
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Human Genome Research Institute (K01HG006699)
  • National Institute of General Medical Sciences (T32GM007183, R37GM037951, GM 37951, DP1GM105386, R01GM037949, R01-GM37949, R01GM037951)
  • National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105386)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
tRNA sequencing, post-transcriptional modification, small RNA profiling, HEK293T cells
MeSH terms
Algorithms, Base Sequence, Humans, Molecular Sequence Data, RNA, Transfer, Gene Library, HEK293 Cells, High-Throughput Nucleotide Sequencing

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