The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections
Abstract
The functions of cortical areas depend on their inputs and outputs, but the detailed circuits made by long-range projections are unknown. We show that the light-gated channel channelrhodopsin-2 (ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2-expressing mice, photostimulation of ChR2-positive axons can be transduced reliably into single action potentials. Combining photostimulation with whole-cell recordings of synaptic currents makes it possible to map circuits between presynaptic neurons, defined by ChR2 expression, and postsynaptic neurons, defined by targeted patching. We applied this technique, ChR2-assisted circuit mapping (CRACM), to map long-range callosal projections from layer (L) 2/3 of the somatosensory cortex. L2/3 axons connect with neurons in L5, L2/3 and L6, but not L4, in both ipsilateral and contralateral cortex. In both hemispheres the L2/3-to-L5 projection is stronger than the L2/3-to-L2/3 projection. Our results suggest that laminar specificity may be identical for local and long-range cortical projections.
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“Furthermore, the EPSCs persisted in the presence of tetradotoxin (TTX) and 4-aminopyridine (4AP), suggesting that they represent monosynaptic glutamate release from DRN DA terminals (Petreanu et al., 2007).”
From Dorsal raphe dopamine neurons represent the experience of social isolation (2016) · cites this paper as Petreanu et al., 2007
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Cites
Millisecond-timescale, genetically targeted optical control of neural activity
Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. A. (2005) · Nature Neuroscience · doi:10.1038/nn1525 · Cited by 5,015
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Synaptic Activity and the Construction of Cortical Circuits
Katz, L. C., & Shatz, C. J. (1996) · Science · doi:10.1126/science.274.5290.1133 · Cited by 3,048
Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., & Miyawaki, A. (2002) · Nature Biotechnology · doi:10.1038/nbt0102-87 · Cited by 2,769
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel
Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold, P. R., Ollig, D., Hegemann, P., & Bamberg, E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1936192100 · Cited by 2,730
NEURONAL CIRCUITS OF THE NEOCORTEX
Douglas, R. J., & Martin, K. A. C. (2004) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.27.070203.144152 · Cited by 1,886
Intrinsic firing patterns of diverse neocortical neurons
Connors, B. W., & Gutnick, M. J. (1990) · Trends in Neurosciences · doi:10.1016/0166-2236(90)90185-d · Cited by 1,440
CHAFFEY, N. J. (2001) · Trends in Plant Science · doi:10.1016/s1360-1385(01)01902-1 · Cited by 1,045
Organizing principles for a diversity of GABAergic interneurons and synapses in the neocortex
Gupta, A., Wang, Y., & Markram, H. (2000) · Science · doi:10.1126/science.287.5451.273 · Cited by 1,036
A Quantitative Map of the Circuit of Cat Primary Visual Cortex
Binzegger, T., Douglas, R. J., & Martin, K. A. C. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.1400-04.2004 · Cited by 893
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Hay, Y. A. (2014) · theses.fr (ABES) · doi:10.70675/e05a8175zd583z4b2ezbb03ze7d3e536abc3
Altwegg‐Boussac, T. (2015) · theses.fr (ABES) · doi:10.70675/b00291fezcae2z498az82e5zaf11f0672b45
Neuronal properties and synaptic connectivity in rodent presubiculum
Simonnet, J. (2014) · theses.fr (ABES) · doi:10.70675/bdae6cc1z374ez4e36z8d20zce45fe5c356e
Anatomie et physiologie des voies de sortie du cervelet chez le rongeur
Aïssa, H. B. (2021) · theses.fr (ABES) · doi:10.70675/892f74c0z4e20z49ddza717zda13327fd474
Advanced optical methods for precise high-throughput in vivo connectivity mapping
Chan, C. Y. (2024) · theses.fr (ABES) · doi:10.70675/98023e25z7828z47fdzab42z41bfd46492af
Tillman, D. E., Durak, O., Veeraraghavan, P., Froberg, J. E., Wheeler, G. C., Budnik, B., & Macklis, J. D. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.08.716720
Althoff, P., Labudda, K., Höweler, U., Lübben, M., Gerwert, K. B., Kötting, C., & Rudack, T. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.02.27.707937
Tanaka, Y., Takahashi, A., Minami, K., Oguma, K., Shimizume, N., Shinozaki, Y., Ozaki, I., & Watanabe, T. (2026) · Scientific Reports · doi:10.1038/s41598-026-52100-x
Herrera, Y. N., Aguirre, B. M., & Roberts, M. T. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.12.724651
Crawley, O., Corral-Sanchez, B., Navarro, A. I., Marco, S., & Pérez‐Otaño, I. (2026) · Journal of Neuroscience · doi:10.1523/jneurosci.0156-25.2026
A hypothalamic VMPO-supraoptic vasopressin circuit mediates procalcitonin-induced fluid imbalance
Lin, W., Liu, T., Huang, J., Li, L., Xu, D., Liu, T., Li, M., Mao, Z., Wen, Y., Guo, J., et al. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.117516
Ge, X., Wei, X., Ruan, B., Wu, Q. Y., Chang, S., Tsai, N. Y., Zhang, S., Duan, X., & Scanziani, M. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.12.731929
Layer 1 NDNF interneurons modulate thalamocortical input to the auditory cortex
Tao, J., Chen, Y., Polat, M., Li, S. C., Chen, X., & Zhao, Z. (2026) · iScience · doi:10.1016/j.isci.2026.116836
Chen, L., Chehade, H. D., Somavarapu, S., & Chu, H. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.07.27.741031
WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit MappingPreprint
Wang, T. A., Chen, C., Liu, R., Yi-Luo, A., Cao, X., Hu, J., Guan, S., Chang, S.-Y., Cui, X., Zhou, W., et al. (2026) · Research Square · doi:10.21203/rs.3.rs-9175553/v1
Inada, K. (2026) · Neuroscience Research · doi:10.1016/j.neures.2026.105109
Michaud, N. M., ten Haaf, M. R., & Crowder, N. A. (2026) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2026.110897
Qi, W., Zhang, X.-Y., Ma, W., Qu, W., Shi, J., Huang, Z., & Zhu, Y. (2026) · CNS Neuroscience & Therapeutics · doi:10.1002/cns.71102
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Chen, L., Chehade, H. D., Somavarapu, S., & Chu, H. (2026) · Research Square · doi:10.21203/rs.3.rs-10703985/v1
Early premotor–somatosensory remodeling after primary motor cortex injury
Nudo, R. J., Barbay, S., Frost, S. B., Taylor, M., Hudson, H. M., & Guggenmos, D. J. (2026) · Cerebral Cortex · doi:10.1093/cercor/bhag145
A cortical–hippocampal communication undergoes rebalancing after new learningPreprint
Hall, A. F., & Wang, D. V. (2026) · eLife · doi:10.7554/elife.107370.2
Serotonin neurons in the dorsal raphe control food-craving-like behavior during pregnancy in mice
Zhao, Q., Feng, B. C., Dong, V., Lau, L., Liu, H., Yu, M., Liang, K., Tran, C., Feng, H., Smiley, T., et al. (2026) · Nature Neuroscience · doi:10.1038/s41593-026-02445-3
A pontomesencephalic-mesolimbic circuit modulates activity-based anorexia
Peng, B., Gao, X., Chen, Y., Xin, Y., Zhang, Y., Yu, K., Lu, M., Li, W., Yang, D., Bao, C., et al. (2026) · Science Advances · doi:10.1126/sciadv.aee1476 · Cited by 1
A basolateral amygdala to dorsolateral striatum projection modulates stimulus-evoked motor behavior
Hobel, Z. B., Brechbill, T. R., Cruz, A. M., Yang, L.-T., Jimenez, K., Wu, B., Liu, Q., Kirsch, S. B., Fernandes, N. J., Bock, R., et al. (2026) · Neuron · doi:10.1016/j.neuron.2026.08.025
Ham, G. X., & Augustine, G. J. (2026) · Cells · doi:10.3390/cells15211937
Metadata
- Authors
- Petreanu, L., Huber, D., Sobczyk, A., & Svoboda, K.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Svoboda, Karel
- Venue
- Nature Neuroscience
- Published
- 2007-04-15
- Volume
- 10
- Issue
- 5
- Pages
- 663-668
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W2163438163
- Cited by
- 930
- References
- 46
- Citations by year
- 2026: 38 · 2025: 38 · 2024: 56 · 2023: 44 · 2022: 56 · 2021: 49 · 2020: 44 · 2019: 40 · 2018: 50 · 2017: 52 · 2016: 54 · 2015: 55 · 2014: 58 · 2013: 57 · 2012: 56
- FWCI
- 15.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Keywords
- channelrhodopsin-2, circuit mapping, somatosensory cortex, layer 2/3 pyramidal neurons, laminar specificity, cortical connectivity
- MeSH terms
- Animals, Animals, Newborn, Axons, Brain Mapping, Corpus Callosum, Embryo, Mammalian, Female, Functional Laterality, Membrane Potentials, Mice, Inbred C57BL, Mice, Transgenic, Nerve Net, Neural Pathways, Photic Stimulation, Pregnancy, Retinaldehyde, Rhodopsin, Somatosensory Cortex, Patch-Clamp Techniques, Mice, In Vitro Techniques
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