Cited by 930Neuroscience and Neuropharmacology ResearchPhotoreceptor and optogenetics researchNeuroscience and Neural Engineering

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Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections

Petreanu, Leopoldo, Huber, Daniel, Sobczyk, Aleksander, Svoboda, Karel·2007·Nature Neuroscience

DOI 10.1038/nn1891

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Abstract

The functions of cortical areas depend on their inputs and outputs, but the detailed circuits made by long-range projections are unknown. We show that the light-gated channel channelrhodopsin-2 (ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2-expressing mice, photostimulation of ChR2-positive axons can be transduced reliably into single action potentials. Combining photostimulation with whole-cell recordings of synaptic currents makes it possible to map circuits between presynaptic neurons, defined by ChR2 expression, and postsynaptic neurons, defined by targeted patching. We applied this technique, ChR2-assisted circuit mapping (CRACM), to map long-range callosal projections from layer (L) 2/3 of the somatosensory cortex. L2/3 axons connect with neurons in L5, L2/3 and L6, but not L4, in both ipsilateral and contralateral cortex. In both hemispheres the L2/3-to-L5 projection is stronger than the L2/3-to-L2/3 projection. Our results suggest that laminar specificity may be identical for local and long-range cortical projections.

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Authors: Petreanu, L., Huber, D., Sobczyk, A., & Svoboda, K.
Affiliations: Howard Hughes Medical Institute (US)
Corresponding author: Svoboda, Karel
ORCID: Petreanu, Leopoldo
Huber, Daniel
Svoboda, Karel
Venue: Nature Neuroscience
Publisher: Springer Science and Business Media LLC
Published: 2007-04-15
Volume: 10
Issue: 5
Pages: 663-668
ISSN: 1097-6256 (ISSN-L)
1546-1726
DOI: 10.1038/nn1891
OpenAlex: W2163438163
Cited by: 930
References: 46
Citations by year: 2026: 38 · 2025: 38 · 2024: 56 · 2023: 44 · 2022: 56 · 2021: 49 · 2020: 44 · 2019: 40 · 2018: 50 · 2017: 52 · 2016: 54 · 2015: 55 · 2014: 58 · 2013: 57 · 2012: 56
FWCI: 15.33
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Neuroscience (publishedVersion)
PubMed (publishedVersion)
Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
Funders: National Institutes of Health
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
Keywords: channelrhodopsin-2, circuit mapping, somatosensory cortex, layer 2/3 pyramidal neurons, laminar specificity, cortical connectivity
MeSH terms: Animals, Animals, Newborn, Axons, Brain Mapping, Corpus Callosum, Embryo, Mammalian, Female, Functional Laterality, Membrane Potentials, Mice, Inbred C57BL, Mice, Transgenic, Nerve Net, Neural Pathways, Photic Stimulation, Pregnancy, Retinaldehyde, Rhodopsin, Somatosensory Cortex, Patch-Clamp Techniques, Mice, In Vitro Techniques

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Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections

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