The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry
Abstract
A novel method for on‐tissue identification of proteins in spatially discrete regions is described using tryptic digestion followed by matrix‐assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) with MS/MS analysis. IMS is first used to reveal the protein and peptide spatial distribution in a tissue section and then a serial section is robotically spotted with small volumes of trypsin solution to carry out in situ protease digestion. After hydrolysis, 2,5‐Dihydroxybenzoic acid (DHB) matrix solution is applied to the digested spots, with subsequent analysis by IMS to reveal the spatial distribution of the various tryptic fragments. Sequence determination of the tryptic fragments is performed using on‐tissue MALDI MS/MS analysis directly from the individual digest spots. This protocol enables protein identification directly from tissue while preserving the spatial integrity of the tissue sample. The procedure is demonstrated with the identification of several proteins in the coronal sections of a rat brain. Copyright © 2007 John Wiley & Sons, Ltd.
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Cites
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
Caprioli, R. M., Farmer, T. B., & Gile, J. (1997) · Analytical Chemistry · doi:10.1021/ac970888i · Cited by 2,234
Self-citationStoeckli, M., Chaurand, P., Hallahan, D. E., & Caprioli, R. M. (2001) · Nature Medicine · doi:10.1038/86573 · Cited by 1,172
Self-citationAssay for the Measurement of Copeptin, a Stable Peptide Derived from the Precursor of Vasopressin
Morgenthaler, N. G., Struck, J., Alonso, C., & Bergmann, A. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2005.060038 · Cited by 1,166
Myelin basic protein: a multifunctional protein
Boggs, J. M. (2006) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-006-6094-7 · Cited by 687
Schwartz, S. A., Reyzer, M. L., & Caprioli, R. M. (2003) · Journal of Mass Spectrometry · doi:10.1002/jms.505 · Cited by 646
Self-citationDirect Molecular Analysis of Whole-Body Animal Tissue Sections by Imaging MALDI Mass Spectrometry
Khatib-Shahidi, S., Andersson, M., Herman, J. L., Gillespie, T. A., & Caprioli, R. M. (2006) · Analytical Chemistry · doi:10.1021/ac060788p · Cited by 505
Self-citationReyzer, M. L., Hsieh, Y., Ng, K., Korfmacher, W. A., & Caprioli, R. M. (2003) · Journal of Mass Spectrometry · doi:10.1002/jms.525 · Cited by 371
Self-citationAutomated Acoustic Matrix Deposition for MALDI Sample Preparation
Aerni, H.-R., Cornett, D. S., & Caprioli, R. M. (2006) · Analytical Chemistry · doi:10.1021/ac051534r · Cited by 344
Self-citationNeurogranin: immunocytochemical localization of a brain-specific protein kinase C substrate
Represa, A., Deloulme, J., Sensenbrenner, M., Ben-Ari, Y., & Baudier, J. (1990) · The Journal of Neuroscience · doi:10.1523/jneurosci.10-12-03782.1990 · Cited by 273
Chaurand, P., Schwartz, S. A., & Caprioli, R. M. (2002) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(02)00370-8 · Cited by 241
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MALDI imaging mass spectrometry of human tissue: method challenges and clinical perspectives
Seeley, E. H., & Caprioli, R. M. (2011) · Trends in Biotechnology · doi:10.1016/j.tibtech.2010.12.002 · Cited by 197
Self-citationRegulation of decellularized matrix mediated immune response
Chakraborty, J., Roy, S., & Ghosh, S. (2020) · Biomaterials Science · doi:10.1039/c9bm01780a · Cited by 130
Metadata
- Authors
- Groseclose, M. R., Andersson, M., Hardesty, W. M., & Caprioli, R. M.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2007-01-17
- Volume
- 42
- Issue
- 2
- Pages
- 254-262
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2021699171
- Cited by
- 377
- References
- 26
- FWCI
- 14.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- Groseclose, M. R., Andersson, M., Hardesty, W. M., & Caprioli, R. M. (2007). Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry. Journal of Mass Spectrometry. https://doi.org/10.1002/jms.1177
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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