Cited by 1,525Pluripotent Stem Cells ResearchMesenchymal stem cell researchCRISPR and Genetic Engineering

The DOI matches its Crossref record; 224 of the 224 references it cites were found in a registry or an index.

Induced pluripotent stem cell technology: a decade of progress.

Open access

Shi, Yanhong, Inoue, Haruhisa, Wu, Joseph C., Yamanaka, Shinya·2017·Nature Reviews Drug Discovery

DOI 10.1038/nrd.2016.245PMC PMC6416143

Open this paper in CoChat — free account

Abstract

Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago, enormous progress has been made in stem cell biology and regenerative medicine. Human iPSCs have been widely used for disease modelling, drug discovery and cell therapy development. Novel pathological mechanisms have been elucidated, new drugs originating from iPSC screens are in the pipeline and the first clinical trial using human iPSC-derived products has been initiated. In particular, the combination of human iPSC technology with recent developments in gene editing and 3D organoids makes iPSC-based platforms even more powerful in each area of their application, including precision medicine. In this Review, we discuss the progress in applications of iPSC technology that are particularly relevant to drug discovery and regenerative medicine, and consider the remaining challenges and the emerging opportunities in the field.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nrd.2016.245

Cites

224 of 224 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Self-citation
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Self-citation
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz‐Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,947
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Genome-scale CRISPR-Cas9 knockout screening in human cells
Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234

Flagged first, then by citations

Show all 224 references

Research works printed but not linked: 1

Meissner, T., Strominger, J. & Cowan, C. The universal donor stem cells: removing the immune barrier to transplantation using CRISPR–Cas9. J. Immunol. 194 (1 Suppl.), 140.128 (2015).

Sorted by citations

Items 301–400 of 1,001

Genetically modified cell sheets in regenerative medicine and tissue engineering
Jiang, Z., Li, N., Zhu, D., Ren, L., Shao, Q., Ke, Y., & Yang, G. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120908 · Cited by 37
Deep Learning‐Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard‐to‐Transfect Cells
Mukherjee, P., Patino, C. A., Pathak, N., Lemaitre, V., & Espinosa, H. D. (2022) · Small · doi:10.1002/smll.202107795 · Cited by 38
Angiogenesis in Regenerative Dentistry: Are We Far Enough for Therapy?
Baru, O., Nutu, A. M., Braicu, C., Cismaru, C. A., Berindan‐Neagoe, I., Buduru, S. D., & Badea, M. E. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22020929 · Cited by 35
Patient-derived organoids for therapy personalization in inflammatory bowel diseases
Lucafò, M., Muzzo, A., Marcuzzi, M., Giorio, L., Decorti, G., & Stocco, G. (2022) · World Journal of Gastroenterology · doi:10.3748/wjg.v28.i24.2636 · Cited by 38
Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy
Chen, H., Yan, G., Wen, M., Brooks, K. N., Zhang, Y., Huang, P.-S., & Chen, T. (2024) · Chemical & Biomedical Imaging · doi:10.1021/cbmi.4c00019 · Cited by 34
The application of in vitro ‐derived human neurons in neurodegenerative disease modeling
D’Souza, G. X., Rose, S. E., Knupp, A., Nicholson, D. A., Keene, C. D., & Young, J. E. (2020) · Journal of Neuroscience Research · doi:10.1002/jnr.24615 · Cited by 36
A novel human pluripotent stem cell-based assay to predict developmental toxicity
Lauschke, K., Rosenmai, A. K., Meiser, I., Neubauer, J. C., Schmidt, K., Rasmussen, M. A., Holst, B., Taxvig, C., Emnéus, J., & Vinggaard, A. M. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02856-6 · Cited by 35
Brain organoids: Human 3D models to investigate neuronal circuits assembly, function and dysfunction
Tambalo, M., & Lodato, S. (2020) · Brain Research · doi:10.1016/j.brainres.2020.147028 · Cited by 36
Magnetic heating of nanoparticles as a scalable cryopreservation technology for human induced pluripotent stem cells
Itô, A., Yoshioka, K., Masumoto, S., Sato, K., Hatae, Y., Nakai, T., Yamazaki, T., Takahashi, M., Tanoue, S., & Horie, M. (2020) · Scientific Reports · doi:10.1038/s41598-020-70707-6 · Cited by 35
Decoding microRNAs in autism spectrum disorder
Li, J., Xu, X., Liu, J., Zhang, S., Tan, X., Li, Z., Zhang, J., & Wang, Z. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.11.005 · Cited by 40
DNA binding redistributes activation domain ensemble and accessibility in pioneer factor Sox2
Bjarnason, S., McIvor, J. A. P., Prestel, A., Demény, K. S., Bullerjahn, J. T., Kragelund, B. B., Mercadante, D., & Heidarsson, P. O. (2024) · Nature Communications · doi:10.1038/s41467-024-45847-2 · Cited by 35
The Potency of Induced Pluripotent Stem Cells in Cartilage Regeneration and Osteoarthritis Treatment
Murphy, C., Mobasheri, A., Táncos, Z., Kobolák, J., & Dinnyés, A. (2017) · Advances in experimental medicine and biology · doi:10.1007/5584_2017_141 · Cited by 34
Integrated generation of induced pluripotent stem cells in a low-cost device
Lin, H., Li, Q., Du, Q., Wang, O., Wang, Z., Akert, L., Carlson, M. A., Zhang, C., Subramanian, A., Zhang, C., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.10.027 · Cited by 35
Hutchinson-Gilford progeria syndrome: Rejuvenating old drugs to fight accelerated ageing
Guilbert, S. M., Cardoso, D., Lévy, N., Muchir, A., & Nissan, X. (2020) · Methods · doi:10.1016/j.ymeth.2020.04.005 · Cited by 31
Microphysiological Modeling of the Human Endometrium
Campo, H., Murphy, A. R., Yıldız, Ş., Woodruff, T. K., Cervelló, I., & Kim, J. J. (2020) · Tissue Engineering Part A · doi:10.1089/ten.tea.2020.0022 · Cited by 36
Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs
Limraksasin, P., Kosaka, Y., Zhang, M., Horie, N., Kondo, T., Okawa, H., Yamada, M., & Egusa, H. (2020) · Scientific Reports · doi:10.1038/s41598-020-72038-y · Cited by 34
Patient-Derived Induced Pluripotent Stem Cells (iPSCs) and Cerebral Organoids for Drug Screening and Development in Autism Spectrum Disorder: Opportunities and Challenges
Villa, C., Combi, R., Conconi, D., & Lavitrano, M. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13020280 · Cited by 37
Induced Pluripotent Stem Cells (iPSCs) and Gene Therapy: A New Era for the Treatment of Neurological Diseases
Sguazzi, G. P., Muto, V., Tartaglia, M., Bertini, E., & Compagnucci, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222413674 · Cited by 37
Direct chemical induction of hepatocyte‐like cells with capacity for liver repopulation
Bai, Y., Yang, Z., Xu, X., Ding, W., Qi, J., Liu, F., Wang, X., Zhou, B., Zhang, W., Zhuang, X., et al. (2022) · Hepatology · doi:10.1002/hep.32686 · Cited by 35
Stem Cell Therapies for Chronic Liver Diseases: Progress and Challenges
Li, T., Wang, Z.-R., Yao, W., Linghu, E., Wang, F., & Shi, L. (2022) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szac053 · Cited by 38
CAR-T State of the Art and Future Challenges, A Regulatory Perspective
Giorgioni, L., Ambrosone, A., Cometa, M. F., Salvati, A. L., & Magrelli, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241411803 · Cited by 37
Myogenic progenitor specification from pluripotent stem cells
Magli, A., & Perlingeiro, R. C. R. (2017) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2017.10.031 · Cited by 34
Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing
Truong, V., Viken, K., Geng, Z., Barkan, S., Johnson, B., Ebeling, M. C., Montezuma, S. R., Ferrington, D. A., & Dutton, J. R. (2021) · SLAS TECHNOLOGY · doi:10.1177/2472630320972110 · Cited by 30
Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
Busch, M., Brouwer, H., Aalderink, G., Bredeck, G., Kämpfer, A. A. M., Schins, R. P. F., & Bouwmeester, H. (2023) · Frontiers in Toxicology · doi:10.3389/ftox.2023.1112212 · Cited by 34
Corneal Endothelial-like Cells Derived from Induced Pluripotent Stem Cells for Cell Therapy
Ng, X. Y., Peh, G. S. L., Yam, G. H., Tay, H. G., & Mehta, J. S. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241512433 · Cited by 34
Advancing therapeutic applications of synthetic gene circuits
Higashikuni, Y., Chen, W. C. W., & Lu, T. K. (2017) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.06.011 · Cited by 32
Clinical Application of iNKT Cell-mediated Anti-tumor Activity Against Lung Cancer and Head and Neck Cancer
Takami, M., Ihara, F., & Motohashi, S. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02021 · Cited by 33
Tissue Engineering for Musculoskeletal Regeneration and Disease Modeling
Li, Z. A., Xiang, S., Li, E. N., Fritch, M. R., Alexander, P. G., Lin, H., & Tuan, R. S. (2020) · Handbook of experimental pharmacology · doi:10.1007/164_2020_377 · Cited by 34
Human induced pluripotent stem cells and CRISPR/Cas-mediated targeted genome editing: Platforms to tackle sensorineural hearing loss
Stojković, M., Han, D., Jeong, M., Stojković, P., & Stanković, K. M. (2021) · Stem Cells · doi:10.1002/stem.3353 · Cited by 34
Bioinspired 3D Culture in Nanoliter Hyaluronic Acid‐Rich Core‐Shell Hydrogel Microcapsules Isolates Highly Pluripotent Human iPSCs
Xu, J., Shamul, J. G., Staten, N. A., White, A. M., Jiang, B., & He, X. (2021) · Small · doi:10.1002/smll.202102219 · Cited by 35
Scalable expansion of iPSC and their derivatives across multiple lineages
Kwok, C. K., Sébastien, I., Hariharan, K., Meiser, I., Wihan, J., Altmaier, S., Karnatz, I., Bauer, D., Fischer, B., Feile, A., et al. (2022) · Reproductive Toxicology · doi:10.1016/j.reprotox.2022.05.007 · Cited by 33
Genetic enhancement: an avenue to combat aging-related diseases
Cai, Y., Ji, Z., Wang, S., Zhang, W., Qu, J. T., Belmonte, J. C. I., & Liu, G. (2022) · Life Medicine · doi:10.1093/lifemedi/lnac054 · Cited by 33
Developing Hypoimmunogenic Human iPSC‐Derived Oligodendrocyte Progenitor Cells as an Off‐The‐Shelf Cell Therapy for Myelin Disorders
Feng, L., Chao, J., Ye, P., Luong, Q., Sun, G., Liu, W., Cui, Q., Flores, S. M., Jackson, N., Shayento, A. N. H., et al. (2023) · Advanced Science · doi:10.1002/advs.202206910 · Cited by 34
Self-citation
New approach methodologies for drug discovery
Liu, W., Pang, P. D., Wu, C. A., Tagle, D. A., & Wu, J. C. (2026) · Cell · doi:10.1016/j.cell.2026.02.012 · Cited by 38
Self-citation
Reimagining human-centric drug development with new approach methodologies
Wu, X., Wu, M. A., Zou, J., Kleinstreuer, N., & Wu, J. C. (2026) · Science · doi:10.1126/science.aeb0045 · Cited by 37
Self-citation
Pluripotent stem cell replacement approaches to treat type 1 diabetes
Pellegrini, S., Piemonti, L., & Sordi, V. (2018) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2018.07.007 · Cited by 31
Systems-Wide Approaches in Induced Pluripotent Stem Cell Models
Lau, E., Paik, D. T., & Wu, J. C. (2018) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathmechdis-012418-013046 · Cited by 33
Self-citation
Cell death assays for neurodegenerative disease drug discovery
Linsley, J. W., Reisine, T., & Finkbeiner, S. (2019) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2019.1623784 · Cited by 32
CRISPR/Cas9-mediated Knockout of the Neuropsychiatric Risk Gene KCTD13 Causes Developmental Deficits in Human Cortical Neurons Derived from Induced Pluripotent Stem Cells
Kizner, V., Naujock, M., Fischer, S. E., Jäger, S., Reich, S., Schlotthauer, I., Zuckschwerdt, K., Geiger, T., Hildebrandt, T., Lawless, N., et al. (2019) · Molecular Neurobiology · doi:10.1007/s12035-019-01727-1 · Cited by 32
Recent advances in models of immune-mediated drug-induced liver injury
Tasnim, F., Huang, X., Lee, C. Z. W., Ginhoux, F., & Yu, H. (2021) · Frontiers in Toxicology · doi:10.3389/ftox.2021.605392 · Cited by 32
Concise Review: Regulatory Influence of Sleep and Epigenetics on Adult Hippocampal Neurogenesis and Cognitive and Emotional Function
Akers, K. G., Chérasse, Y., Fujita, Y., Srinivasan, S., Sakurai, T., & Sakaguchi, M. (2018) · Stem Cells · doi:10.1002/stem.2815 · Cited by 34
Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
Kim, J., Kim, Y., Choi, J., Jung, H., Lee, K.-J., Kang, J., Park, N., Rim, Y. A., Nam, Y., & Ju, J. H. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-1100-1 · Cited by 32
A decade of liver organoids: Advances in disease modeling
Liu, Y., Sheng, J.-Y., Yang, C.-F., Ding, J., & Chan, Y.-S. (2023) · Clinical and Molecular Hepatology · doi:10.3350/cmh.2022.0428 · Cited by 30
Whole-Heart Tissue Engineering and Cardiac Patches: Challenges and Promises
Akbarzadeh, A., Sobhani, S., Khaboushan, A. S., & Kajbafzadeh, A. M. (2023) · Bioengineering · doi:10.3390/bioengineering10010106 · Cited by 32
Novel therapeutic agents for management of diabetes mellitus: A hope for drug designing against diabetes mellitus
Elkhalifa, A. M. E., Nazar, M., Ali, S. I., Khursheed, I., Taifa, S., Ahmad Mir, M., Shah, I. H., Malik, M., Ramzan, Z., Ahad, S., et al. (2024) · Life · doi:10.3390/life14010099 · Cited by 31
Emerging technologies in regenerative medicine: The future of wound care and therapy
Sharma, Y., Ghatak, S., Sen, C. K., & Mohanty, S. (2024) · Journal of Molecular Medicine · doi:10.1007/s00109-024-02493-x · Cited by 32
Contribution of induced pluripotent stem cell technologies to the understanding of cellular phenotypes in schizophrenia
Balan, S., Toyoshima, M., & Yoshikawa, T. (2018) · Neurobiology of Disease · doi:10.1016/j.nbd.2018.04.021 · Cited by 31
Regenerating the kidney using human pluripotent stem cells and renal progenitors
Becherucci, F., Mazzinghi, B., Allinovi, M., Angelotti, M. L., & Romagnani, P. (2018) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2018.1492546 · Cited by 30
Application of induced pluripotent stem cell transplants: Autologous or allogeneic?
Li, C., Chen, S., Zhou, Y., Zhao, Y., Liu, P., & Cai, J. (2018) · Life Sciences · doi:10.1016/j.lfs.2018.09.057 · Cited by 31
Various strategies to improve efficacy of stem cell transplantation in multiple sclerosis: Focus on mesenchymal stem cells and neuroprotection
Yousefi, F., Arab, F. L., Saeidi, K., Amiri, H., & Mahmoudi, M. (2018) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2018.11.015 · Cited by 34
Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications
Cotovio, J. P., & Fernandes, T. G. (2020) · Bioengineering · doi:10.3390/bioengineering7020036 · Cited by 31
The Progress and Clinical Application of Breast Cancer Organoids
Yu, J. S., & Huang, W. (2020) · International Journal of Stem Cells · doi:10.15283/ijsc20082 · Cited by 32
Cell‐Based Therapy for Canavan Disease Using Human iPSC‐Derived NPCs and OPCs
Feng, L., Chao, J., Tian, E., Li, L., Ye, P., Zhang, M., Chen, X., Cui, Q., Sun, G., Zhou, T., et al. (2020) · Advanced Science · doi:10.1002/advs.202002155 · Cited by 30
Self-citation
Generation and Culture of Cardiac Microtissues in a Microfluidic Chip with a Reversible Open Top Enables Electrical Pacing, Dynamic Drug Dosing and Endothelial Cell Co‐Culture
Vivas, A., IJspeert, C., Pan, J. Y., Vermeul, K., van den Berg, A., Passier, R. C., Keller, S. S., & van der Meer, A. D. (2022) · Advanced Materials Technologies · doi:10.1002/admt.202101355 · Cited by 31
Neuronal Cultures: Exploring Biophysics, Complex Systems, and Medicine in a Dish
Soriano, J. (2023) · Biophysica · doi:10.3390/biophysica3010012 · Cited by 33
Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model
Liu, Z., Chao, J., Wang, C., Sun, G., Roeth, D., Liu, W., Chen, X., Li, L., Tian, E., Feng, L., et al. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112841 · Cited by 30
Self-citation
Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells
Yi, H., Xie, B., Liu, B., Wang, X., Xu, L., Liu, J., Li, M., Zhong, X., & Peng, F. (2018) · Stem Cells International · doi:10.1155/2018/3628578 · Cited by 32
Integration of Lead Discovery Tactics and the Evolution of the Lead Discovery Toolbox
Leveridge, M. V., Chung, C., Gross, J. W., Phelps, C. B., & Green, D. V. S. (2018) · SLAS DISCOVERY · doi:10.1177/2472555218778503 · Cited by 32
Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays
Hartmann, J., Henschel, N., Bartmann, K., Dönmez, A., Brockerhoff, G., Koch, K., & Fritsche, E. (2023) · Cells · doi:10.3390/cells12091270 · Cited by 31
Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era
Singh, B., Mal, G., Verma, V., Tiwari, R., Khan, M. I., Mohapatra, R. K., Mitra, S., Alyami, S. A., Emran, T. B., Dhama, K., et al. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02334-5 · Cited by 30
Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review
Lv, S., He, E., Luo, J., Liu, Y., Liang, W. J., Xu, S., Zhang, K., Yang, Y. F., Wang, M., Song, Y., et al. (2023) · Advanced Science · doi:10.1002/advs.202301828 · Cited by 32
Generation of hypoimmunogenic universal iPS cells through HLA-type gene knockout
Kim, J., Nam, Y., Jeon, D., Choi, Y.-J., Choi, S., Hong, C. P., Kim, S., Jung, H., Park, N., Sohn, Y., et al. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01422-3 · Cited by 36
Rethinking Regenerative Medicine From a Transplant Perspective (and Vice Versa)
Orlando, G., Murphy, S. V., Bussolati, B., Clancy, M. J., Cravedi, P., Migliaccio, G., & Murray, P. A. (2018) · Transplantation · doi:10.1097/tp.0000000000002370 · Cited by 31
3D Bioprinting of Adipose-Derived Stem Cells for Organ Manufacturing
Wang, X., & Liu, C. (2018) · Advances in experimental medicine and biology · doi:10.1007/978-981-13-0950-2_1 · Cited by 30
A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons
Stathakos, P., Jiménez-Moreno, N., Crompton, L., Nistor, P., Badger, J. L., Barbuti, P. A., Kerrigan, T. L., Randall, A. D., Caldwell, M. A., & Lane, J. D. (2020) · Autophagy · doi:10.1080/15548627.2020.1739441 · Cited by 31
Biallelic correction of sickle cell disease-derived induced pluripotent stem cells (iPSCs) confirmed at the protein level through serum-free iPS-sac/erythroid differentiation
Haro‐Mora, J. J., Uchida, N., Demirci, S., Wang, Q., Zou, J., & Tisdale, J. F. (2020) · Stem Cells Translational Medicine · doi:10.1002/sctm.19-0216 · Cited by 30
Current understanding of and emerging treatment options for spinal muscular atrophy with respiratory distress type 1 (SMARD1)
Perego, M. G. L., Galli, N., Nizzardo, M., Govoni, A., Taiana, M. M., Bresolin, N., Comi, G. P., & Corti, S. P. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03492-0 · Cited by 30
Mesenchymal stromal cells for articular cartilage repair: preclinical studies
Fernández‐Pernas, P., Barrachina, L., Marquina, M., Rodellar, C., Arufe, M. C., & Costa, C. (2020) · European Cells and Materials · doi:10.22203/ecm.v040a06 · Cited by 30
Experimental Models to Study Autism Spectrum Disorders: hiPSCs, Rodents and Zebrafish
Pensado‐López, A., Veiga-Rúa, S., Carracedo, Á., Allegue, C., & Sanchez, L. E. (2020) · Genes · doi:10.3390/genes11111376 · Cited by 34
Techno-Economic Analysis of Automated iPSC Production
Nießing, B., Kiesel, R., Herbst, L., & Schmitt, R. H. (2021) · Processes · doi:10.3390/pr9020240 · Cited by 32
Hair Follicle Stem Cells for Tissue Regeneration
Peterson, A., & Nair, L. S. (2021) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2021.0098 · Cited by 30
MicroRNA Roles in Cell Reprogramming Mechanisms
Pascale, E., Caiazza, C., Paladino, M., Parisi, S., Passaro, F., & Caiazzo, M. (2022) · Cells · doi:10.3390/cells11060940 · Cited by 30
Super-enhancers conserved within placental mammals maintain stem cell pluripotency
Zhang, J., Zhou, Y., Yue, W., Zhu, Z., Wu, X., Yu, S., Shen, Q., Pan, Q., Xu, W., Zhang, R., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2204716119 · Cited by 27
Recognizing the Differentiation Degree of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells Using Machine Learning and Deep Learning-Based Approaches
Lien, C.-Y., Chen, T.-T., Tsai, E.-T., Hsiao, Y., Lee, N., Gao, C.-E., Yang, Y., Chen, S., Yarmishyn, A. A., Hwang, D., et al. (2023) · Cells · doi:10.3390/cells12020211 · Cited by 30
Exploiting urine-derived induced pluripotent stem cells for advancing precision medicine in cell therapy, disease modeling, and drug testing
Yin, X., Li, Q., Shu, Y., Wang, H., Thomas, B., Maxwell, J. T., & Zhang, Y. (2024) · Journal of Biomedical Science · doi:10.1186/s12929-024-01035-4 · Cited by 37
Cell therapy for liver disorders: past, present and future
Ortuño‐Costela, M. D. C., Pinzani, M., & Vallier, L. (2025) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-025-01050-2 · Cited by 34
Interrupted reprogramming of alveolar type II cells induces progenitor-like cells that ameliorate pulmonary fibrosis
Guo, L., Karoubi, G., Duchesneau, P., Aoki, F. G., Shutova, M. V., Rogers, I. M., Nagy, A., & Waddell, T. K. (2018) · npj Regenerative Medicine · doi:10.1038/s41536-018-0052-5 · Cited by 29
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Kim, Y., & Ju, J. H. (2019) · Journal of Visualized Experiments · doi:10.3791/59297 · Cited by 31
The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues
Ogoke, O., Maloy, M. H., & Parashurama, N. (2020) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12650 · Cited by 28
DNA methylation and the core pluripotency network
Shanak, S., & Helms, V. (2020) · Developmental Biology · doi:10.1016/j.ydbio.2020.06.001 · Cited by 31
Advances in Regeneration of Retinal Ganglion Cells and Optic Nerves
Yuan, F., Wang, M., Jin, K., & Xiang, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094616 · Cited by 30
Targeting NMDA receptors in neuropsychiatric disorders by drug screening on human neurons derived from pluripotent stem cells
Zhang, W., Ross, P. J., Ellis, J., & Salter, M. W. (2022) · Translational Psychiatry · doi:10.1038/s41398-022-02010-z · Cited by 36
Human mini-blood–brain barrier models for biomedical neuroscience research: a review
Tran, M., Heo, C., Lee, L. P., & Cho, H. (2022) · Biomaterials Research · doi:10.1186/s40824-022-00332-z · Cited by 31
Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective
Yousafzai, M. S., & Hammer, J. A. (2023) · Biosensors · doi:10.3390/bios13100905 · Cited by 30
Self-Organized Cerebellar Tissue from Human Pluripotent Stem Cells and Disease Modeling with Patient-Derived iPSCs
Muguruma, K. (2017) · The Cerebellum · doi:10.1007/s12311-017-0905-2 · Cited by 29
Safe scarless cassette-free selection of genome-edited human pluripotent stem cells using temporary drug resistance
Kim, K.-T., Park, J.-C., Jang, H., Lee, H., Park, S., Kim, J., Kwon, O., Go, Y.-H., Jin, Y., Kim, W., et al. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120295 · Cited by 26
Application of patient-derived induced pluripotent stem cells and organoids in inherited retinal diseases
Liang, Y., Sun, X., Duan, C., Tang, S., & Chen, J. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03564-5 · Cited by 28
Directing neuronal cell fate in vitro: Achievements and challenges
Riemens, R. J. M., van den Hove, D., Esteller, M., & Delgado‐Morales, R. (2018) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2018.04.003 · Cited by 27
Epigenetic Enzymes, Age, and Ancestry Regulate the Efficiency of Human iPSC Reprogramming
Mackey, L. C., Annab, L. A., Yang, J., Rao, B., Kissling, G. E., Schurman, S. H., Dixon, D., & Archer, T. (2018) · Stem Cells · doi:10.1002/stem.2899 · Cited by 28
Modeling Human Brain Circuitry Using Pluripotent Stem Cell Platforms
Hartlaub, A. M., McElroy, C. A., Maitre, N. L., & Hester, M. E. (2019) · Frontiers in Pediatrics · doi:10.3389/fped.2019.00057 · Cited by 28
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications
Tian, S., Feng, J., Cao, Y., Shen, S., Cai, Y., Yang, D., Yan, R., Wang, L., Zhang, H., Zhong, X., et al. (2019) · Life Science Alliance · doi:10.26508/lsa.201900413 · Cited by 26
Towards the clinical translation of optogenetic skeletal muscle stimulation
Gundelach, L. A., Hüser, M. A., Beutner, D., Ruther, P., & Bruegmann, T. (2020) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-020-02387-0 · Cited by 30
Biological implications of genetic variations in autism spectrum disorders from genomics studies
Zhang, Y., Liu, X., Guo, R., Xu, W., Guo, Q. J., Hao, C., Ni, X., & Li, W. (2021) · Bioscience Reports · doi:10.1042/bsr20210593 · Cited by 33
Approaches in Immunotherapy, Regenerative Medicine, and Bioengineering for Type 1 Diabetes
Kopan, C., Tucker, T. R., Alexander, M., Mohammadi, M. R., Pone, E. J., & Lakey, J. R. T. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01354 · Cited by 28
Synthetic Biology and Tissue Engineering: Toward Fabrication of Complex and Smart Cellular Constructs
Hoffman, T., Antovski, P., Tebon, P. J., Xu, C., Ashammakhi, N. A., Ahadian, S., Morsut, L., & Khademhosseini, A. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201909882 · Cited by 32
Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives
Zhu, Z., Cheng, Y., Liu, X., Ding, W., Liu, J., Ling, Z., & Wu, L. (2025) · Cell Transplantation · doi:10.1177/09636897241303271 · Cited by 31
Deciphering Cell Intrinsic Properties: A Key Issue for Robust Organoid Production
Picollet-D’hahan, N., Dolega, M. E., Freida, D., Martin, D. K., & Gidrol, X. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.08.003 · Cited by 36
Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response
Saygılı, E., Yıldız-Ozturk, E., Green, M. J., Ghaemmaghami, A. M., & Yeşil-Çeliktaş, Ö. (2021) · Biomicrofluidics · doi:10.1063/5.0038924 · Cited by 27
Human iPSC-based neurodevelopmental models of globoid cell leukodystrophy uncover patient- and cell type-specific disease phenotypes
Mangiameli, E., Cecchele, A., Morena, F., Sanvito, F., Matafora, V., Cattaneo, A., della Volpe, L., Gnani, D., Paulis, M., Susani, L., et al. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.04.011 · Cited by 27
Derivation of male germ cells from induced pluripotent stem cells by inducers: A review
Mahabadi, J. A., Sabzalipoor, H., Kehtari, M., Enderami, S. E., Soleimani, M., & Nikzad, H. (2018) · Cytotherapy · doi:10.1016/j.jcyt.2018.01.002 · Cited by 27

Authors: Shi, Y., Inoue, H., Wu, J. C., & Yamanaka, S.
Affiliations: City Of Hope National Medical Center (US)
Kyoto University (JP)
Stanford Cardiovascular Institute (US)
Gladstone Institutes (US)
Corresponding author: Shi, Yanhong
Inoue, Haruhisa
ORCID: Shi, Yanhong
Inoue, Haruhisa
Wu, Joseph C.
Yamanaka, Shinya
Venue: Nature Reviews Drug Discovery
Publisher: Springer Science and Business Media LLC
Published: 2016-12-16
Volume: 16
Issue: 2
Pages: 115-130
ISSN: 1474-1776 (ISSN-L)
1474-1784
DOI: 10.1038/nrd.2016.245
PMC: PMC6416143
OpenAlex: W2565787913
Cited by: 1,525
References: 224
Citations by year: 2026: 96 · 2025: 143 · 2024: 177 · 2023: 185 · 2022: 194 · 2021: 205 · 2020: 199 · 2019: 123 · 2018: 143 · 2017: 56 · 2016: 1
FWCI: 9.72
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: www.springer.com/tdm (tdm)
Locations: Nature Reviews Drug Discovery (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: National Heart, Lung, and Blood Institute (R01HL128170, R01HL130020)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords: induced pluripotent stem cells, drug discovery, regenerative medicine, disease modeling, cell therapy, genome editing, 3D organoids, precision medicine, clinical trials
MeSH terms: Drug Therapy, Humans, Stem Cell Transplantation, Regenerative Medicine, History, 21st Century, Drug Discovery, Induced Pluripotent Stem Cells, High-Throughput Screening Assays

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Induced pluripotent stem cell technology: a decade of progress.

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks