The DOI matches its Crossref record; 78 of the 78 references it cites were found in a registry or an index.
High-throughput RNAi screening in cultured cells: a user's guide
Open accessAbstract
RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.
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“High-throughput screening techniques that can generate large amounts of data are now common in many fields of biology”
From WILDS: A Benchmark of in-the-Wild Distribution Shifts (2020) · cites this paper as Echeverri and Perrimon, 2006
Citations
Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
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The nuclear RNase III Drosha initiates microRNA processing
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A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants
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The Microprocessor complex mediates the genesis of microRNAs
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Nuclear Export of MicroRNA Precursors
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Denli, A. M., Tops, B. B. J., Plasterk, R. H. A., Ketting, R. F., & Hannon, G. J. (2004) · Nature · doi:10.1038/nature03049 · Cited by 2,784
Expression profiling reveals off-target gene regulation by RNAi
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TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
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Research works printed but not linked: 1
- Echalier, G. Drosophila Cell in Culture (Academic Press, New York, 1997).
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Carpenter, A. E., Jones, T. R., Lamprecht, M. R., Clarke, C., Kang, I. H., Friman, O., Guertin, D. A., Chang, J. H., Lindquist, R. A., Moffat, J., et al. (2006) · Genome biology · doi:10.1186/gb-2006-7-10-r100 · Cited by 5,212
Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Joung, J., Konermann, S., Gootenberg, J. S., Abudayyeh, O. O., Platt, R. J., Brigham, M. D., Sanjana, N. E., & Zhang, F. (2017) · Nature Protocols · doi:10.1038/nprot.2017.016 · Cited by 1,243
High-throughput functional genomics using CRISPR-Cas9
Shalem, O., Sanjana, N. E., & Zhang, F. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3899 · Cited by 1,233
Gandre-Babbe, S., & van der Bliek, A. M. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-12-1287 · Cited by 769
Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI
Hogan, P. G., Lewis, R. S., & Rao, A. (2010) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132550 · Cited by 769
Metadata
- Authors
- Echeverri, C. J., & Perrimon, N.
- Affiliations
- Cenix BioScience (Germany) (DE)
- Howard Hughes Medical Institute (US)
- ORCID
- Perrimon, Norbert
- Venue
- Nature Reviews Genetics
- Published
- 2006-04-11
- Volume
- 7
- Issue
- 5
- Pages
- 373-384
- ISSN
- 1471-0056 (ISSN-L)
- 1471-0064
- OpenAlex
- W1968410036
- Cited by
- 380
- References
- 78
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 12 · 2023: 7 · 2022: 7 · 2021: 10 · 2020: 15 · 2019: 10 · 2018: 13 · 2017: 14 · 2016: 18 · 2015: 17 · 2014: 25 · 2013: 20 · 2012: 23
- FWCI
- 3.08
- Type
- journal article
- Language
- en
- Open access
- green
- nrs.harvard.edu
- Licence
- Locations
- Nature Reviews Genetics (publishedVersion)
- PubMed (publishedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- RNA interference, high-throughput screening, loss-of-function screen, functional genomics, cell lines, screening optimization, quality control, normalization strategies, control system design
- MeSH terms
- Animals, Automation, Cells, Cultured, Drosophila melanogaster, Humans, Models, Biological, Phenotype, Quality Control, Gene Transfer Techniques, Gene Expression Profiling, RNA Interference, RNA, Small Interfering
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