High-throughput RNAi screening in cultured cells: a user's guide

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

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A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.

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The nuclear RNase III Drosha initiates microRNA processing

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A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants

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The Microprocessor complex mediates the genesis of microRNAs

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Nuclear Export of MicroRNA Precursors

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Processing of primary microRNAs by the Microprocessor complex

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Expression profiling reveals off-target gene regulation by RNAi

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TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing

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Rational siRNA design for RNA interference

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Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

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Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells

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Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs

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Activation of the interferon system by short-interfering RNAs

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Regulation by let-7 and lin-4 miRNAs Results in Target mRNA Degradation

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siRNAs can function as miRNAsPreprint

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Cell lines derived from late embryonic stages of Drosophila melanogaster

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A large-scale RNAi screen in human cells identifies new components of the p53 pathway

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Microarrays of cells expressing defined cDNAs

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3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

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Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

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Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy

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The Human DiGeorge Syndrome Critical Region Gene 8 and Its D. melanogaster Homolog Are Required for miRNA Biogenesis

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Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

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Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways

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Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

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A resource for large-scale RNA-interference-based screens in mammals

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Second-generation shRNA libraries covering the mouse and human genomes

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Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells

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Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis

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Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance

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Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein

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Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells

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A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells

Stegmeier, F. P., Hu, G., Rickles, R. J., Hannon, G. J., & Elledge, S. J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506306102 · Cited by 530

Probing tumor phenotypes using stable and regulated synthetic microRNA precursors

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A Genetic Screen for Candidate Tumor Suppressors Identifies REST

Westbrook, T. F., Martin, E. S., Schlabach, M. R., Leng, Y., Liang, A. C. T., Feng, B., Zhao, J. J., Roberts, T. M., Mandel, G., Hannon, G. J., et al. (2005) · Cell · doi:10.1016/j.cell.2005.03.033 · Cited by 492

Analysis of Clathrin-mediated Endocytosis of Epidermal Growth Factor Receptor by RNA Interference

Huang, F., Khvorova, A., Marshall, W., & Sorkin, A. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.c400046200 · Cited by 470

Processing of Pre-microRNAs by the Dicer-1–Loquacious Complex in Drosophila Cells

Saito, K., Ishizuka, A., Siomi, H., & Siomi, M. C. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030235 · Cited by 450

A functional genomic analysis of cell morphology using RNA interference

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An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

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In vitrol culture ofDrosophila melanogaster embryonic cells

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Design of a genome-wide siRNA library using an artificial neural network

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Drosophila RNAi Screen Reveals CD36 Family Member Required for Mycobacterial Infection

Philips, J. A., Rubin, E. J., & Perrimon, N. (2005) · Science · doi:10.1126/science.1116006 · Cited by 381

Activation of the mammalian immune system by siRNAs

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Genome-wide survey of protein kinases required for cell cycle progression

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Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway

DasGupta, R., Kaykas, A., Moon, R. T., & Perrimon, N. (2005) · Science · doi:10.1126/science.1109374 · Cited by 333

Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets

Eggert, U., Kiger, A. A., Richter, C., Perlman, Z. E., Perrimon, N., Mitchison, T. J., & Field, C. M. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020379 · Cited by 325

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells

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Genome-Wide RNAi Screen for Host Factors Required for Intracellular Bacterial Infection

Agaisse, H., Burrack, L. S., Philips, J. A., Rubin, E. J., Perrimon, N., & Higgins, D. E. (2005) · Science · doi:10.1126/science.1116008 · Cited by 308

Identification of JAK/STAT signalling components by genome-wide RNA interference

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Systematic genome-wide screens of gene function

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A Genetic Screen Identifies PITX1 as a Suppressor of RAS Activity and Tumorigenicity

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Functional Dissection of an Innate Immune Response by a Genome-Wide RNAi Screen

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Sustained Small Interfering RNA-Mediated HumanImmunodeficiency Virus Type 1 Inhibition in PrimaryMacrophages

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Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibitionPreprint

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Genome-wide RNAi analysis of JAK/STAT signaling components in DrosophilaPreprint

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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes

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The ferredoxin reductase gene is regulated by the p53 family and sensitizes cells to oxidative stress-induced apoptosis

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RNAi Microarray Analysis in Cultured Mammalian CellsPreprint

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A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway

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Identification and Characterization of a Novel Line of Drosophila Schneider S2 Cells That Respond to Wingless Signaling

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Functional genomics to new drug targets

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RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells

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An RNA interference screen ide.jpgies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila

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RNAi living-cell microarrays for loss-of-function screens in Drosophila melanogaster cells

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Microarrays of lentiviruses for gene function screens in immortalized and primary cells

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High-throughput RNAi screening in vitro: From cell lines to primary cells

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A TRAIL receptor-dependent synthetic lethal relationship between MYC activation and GSK3β/FBW7 loss of function

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Involvement of MINK, a Ste20 Family Kinase, in Ras Oncogene-Induced Growth Arrest in Human Ovarian Surface Epithelial Cells

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In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5

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Newly established cell lines fromDrosophila larval CNS express neural specific characteristics

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RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells

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High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells

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siRNA Cell Arrays for High-Content Screening Microscopy

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Genes required for Drosophila nervous system development identified by RNA interference

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The ultrastructure of imaginal disc cells in primary cultures and during cell aggregation in continuous cell lines

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Drosophila Cell Culture

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CellProfiler: image analysis software for identifying and quantifying cell phenotypes

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Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

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High-throughput functional genomics using CRISPR-Cas9

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The Novel Tail-anchored Membrane Protein Mff Controls Mitochondrial and Peroxisomal Fission in Mammalian Cells

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Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI

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High‐throughput RNAi screening to dissect cellular pathways: A how‐to guide

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High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways

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RNA interference in mammals: behind the screen

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High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells

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An Analysis of Normalization Methods for Drosophila RNAi Genomic Screens and Development of a Robust Validation Scheme

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