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The art and design of genetic screens: Drosophila melanogaster

St Johnston, Daniel·2002·Nature Reviews Genetics
DOI 10.1038/nrg751
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Abstract

The success of Drosophila melanogaster as a model organism is largely due to the power of forward genetic screens to identify the genes that are involved in a biological process. Traditional screens, such as the Nobel-prize-winning screen for embryonic-patterning mutants, can only identify the earliest phenotype of a mutation. This review describes the ingenious approaches that have been devised to circumvent this problem: modifier screens, for example, have been invaluable for elucidating signal-transduction pathways, whereas clonal screens now make it possible to screen for almost any phenotype in any cell at any stage of development.

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Metadata

Authors
St Johnston, D.
Venue
Nature Reviews Genetics
Publisher
Springer Science and Business Media LLC
Published
2002-03
Volume
3
Issue
3
Pages
176-188
ISSN
  • 1471-0056 (ISSN-L)
  • 1471-0064
DOI
10.1038/nrg751
OpenAlex
W2027125425
Cited by
644
References
0
Citations by year
2026: 7 · 2025: 17 · 2024: 26 · 2023: 23 · 2022: 25 · 2021: 28 · 2020: 40 · 2019: 33 · 2018: 38 · 2017: 29 · 2016: 30 · 2015: 40 · 2014: 41 · 2013: 32 · 2012: 36
FWCI
1.76
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Reviews Genetics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Diptera species taxonomy and behavior
Keywords
forward genetic screen, Drosophila melanogaster, modifier screen, embryonic patterning, signaling pathways
MeSH terms
Animals, Crosses, Genetic, Drosophila melanogaster, Enhancer Elements, Genetic, Eye, Female, Genetic Testing, Male, Phenotype, Gene Expression, Genes, Suppressor, Mutagenesis, Genes, Insect

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