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TALENs: a widely applicable technology for targeted genome editing
Open accessAbstract
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.
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“Each repeat binds to a single nucleotide, and the amino acids residues in the positions 12 and 13 of that repeat determine which nucleotide is bound [91].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [91]
In the citing paper
“The discovery that TALEs use a simple modular code for DNA recognition has provided an alternative platform for genome editing and gene disruption.”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [66]
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Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U. (2009) · Science · doi:10.1126/science.1178811 · Cited by 2,470 · cited 1×
“21 The TALE repeat code was also confirmed by another group by performing a computational analysis of the binding specificities of naturally occurring TALEs 22 .”
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
A TALE nuclease architecture for efficient genome editing
Miller, J. C., Tan, S., Qiao, G., Barlow, K. A., Wang, J., Xia, D. F., Meng, X., Paschon, D. E., Leung, E., Hinkley, S. J., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1755 · Cited by 2,098 · cited 7×
“Alternatively, precise insertions have also been introduced into endogenous human genes using HDR with TALENs and a double-stranded homologous donor template plasmid 19, 20 .”
InfluentialA Simple Cipher Governs DNA Recognition by TAL Effectors
Moscou, M., & Bogdanove, A. J. (2009) · Science · doi:10.1126/science.1178817 · Cited by 2,050 · cited 6×
“4 DNA binding by these TALEs is mediated by arrays of highly conserved 33–35 amino acid repeats flanked by additional TALE-derived domains at the amino-and carboxy-terminal ends of the array (Figure 1c).”
InfluentialČermák, T., Doyle, E., Christian, M. L., Wang, L., Zhang, Y., Schmidt, C. L., Baller, J. A., Somia, N. V., Bogdanove, A. J., & Voytas, D. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr218 · Cited by 2,034 · cited 6×
“To date, TALENs have been used to make knockout mutations in Arabidopsis 15 and to introduce resistance to infection by Xanthomonas bacteria in rice by disrupting the target sites of naturally occurring TALEs that contribute to pathogenicity 16 .”
InfluentialHybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.
Kim, Y. G., Cha, J., & Chandrasegaran, S. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1156 · Cited by 1,889 · cited 1×
“A large number of zinc finger arrays engineered using these various methods have been fused to a non-specific nuclease domain from the Type IIS FokI restriction enzyme to create zinc finger nucleases ( ZFN s) 91 .”
Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Christian, M., Cermak, T., Doyle, E. L., Schmidt, C., Zhang, F., Hummel, A., Bogdanove, A. J., & Voytas, D. F. (2010) · Genetics · doi:10.1534/genetics.110.120717 · Cited by 1,869 · cited 1×
“24 The TALEN framework was then refined by showing that nuclease activities could be greatly enhanced by truncating the length of this carboxy-terminal TALE-derived sequence 19 , 68 .”
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Urnov, F. D., Miller, J. C., Lee, Y.-L., Beausejour, C. M., Rock, J. M., Augustus, S., Jamieson, A. C., Porteus, M. H., Gregory, P. D., & Holmes, M. C. (2005) · Nature · doi:10.1038/nature03556 · Cited by 1,642 · cited 1×
“…38 , catfish 39 , sea urchin 40 , frog 41 , pig 42 , cattle 43 , cricket 8 , rabbit 44 , silkworm 45 , butterfly 46 , mouse 47, 48 , rat 49, 50 , soybean 51, 52 , thale cress 53, 54 , corn 55 , tobacco 56 , petunia 57 , hamster cells 58 , and human somatic 59, 60 and pluripotent stem cells 61–63 .”
Vascular Endothelial Growth Factor
Ferrara, N. (2009) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.108.179663 · Cited by 1,308
FLASH assembly of TALENs for high-throughput genome editing
Reyon, D., Tsai, S. Q., Khayter, C., Foden, J. A., Sander, J. D., & Joung, J. K. (2012) · Nature Biotechnology · doi:10.1038/nbt.2170 · Cited by 1,171 · cited 6×
“Although the high success rate and robust targeting range of dimeric TALENs has been recently established, 18 relatively less is known about these parameters for monomeric engineered TALE-based transcriptional activators or repressors.”
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Wang, J., Yang, J., Li, D., & Li, J. (2020) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2020.188454 · Cited by 53
Mitochondrial Heteroplasmy Shifting as a Potential Biomarker of Cancer Progression
Pérez-Amado, C. J., Bazan-Cordoba, A., Hidalgo‐Miranda, A., & Jiménez‐Morales, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147369 · Cited by 54
Müller, T. M., Böttcher, C., Morbitzer, R., Götz, C. C., Lehmann, J. C., Lahaye, T., & Glawischnig, E. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00481 · Cited by 51
“We used TRANSCRIPTION ACTIVATORLIKE EFFECTOR NUCLEASEs (TALENs; Christian et al., 2010; Joung and Sander, 2013) to create stable cyp71a12 cyp71a13 double knockout lines.”
BackgroundMethodDetermining causality and consequence of expression quantitative trait loci
Battle, A. J., & Montgomery, S. B. (2014) · Human Genetics · doi:10.1007/s00439-014-1446-0 · Cited by 53
“2013) and transcription activator-like effector nucleases (TaLeNs) (Joung and Sander 2013).”
BackgroundEvolution of egg coats: linking molecular biology and ecology
Shu, L., Suter, M. J. ‐., & Räsänen, K. (2015) · Molecular Ecology · doi:10.1111/mec.13283 · Cited by 51
“…genome editing techniques, such as zinc-finger nucleases (ZFNs) (Carroll 2011), transcription activator-like effector nucleases (TALENs) (Joung & Sander 2013) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based system (Sander © 2015 John Wiley & Sons…”
MethodTargeted Genome Editing in Human Cells Using CRISPR/Cas Nucleases and Truncated Guide RNAs
Fu, Y., Reyon, D., & Joung, J. K. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-801185-0.00002-7 · Cited by 50
Cheng, W.-J., Chen, L.-C., Ho, H., Lin, H.-L., & Sheu, M. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s181440 · Cited by 51
Lux, C. T., Pattabhi, S., Berger, M. P., Nourigat, C., Flowers, D. A., Nègre, O., Humbert, O., Yang, J., Lee, C., Jacoby, K., et al. (2018) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2018.12.008 · Cited by 52
“DNA binding is paired with endonuclease cleavage via use of a FokI nuclease fused to each of the respective DNA-binding domains requiring two unique DNA binding sites in a defined proximity leading to a low degree of off-target cutting.(19) TALEN delivery as mRNA avoids the requirement for viral delivery or for protei…”
Gene Therapy for Cardiovascular Disease: Basic Research and Clinical Prospects
Cao, G., Xuan, X., Zhang, R., Hu, J. H., & Dong, H. (2021) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.760140 · Cited by 58
“As FokI nuclease requires homodimerization for activate cleavage, a pair of TALENs were designed to locate at the upstream and downstream sequences of the target site (32).”
BackgroundRazzaq, M. K., Akhter, M., Ahmad, R. M., Cheema, K. L., Hina, A., Karikari, B., Raza, G., Xing, G., Gai, J., & Khurshid, M. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-07391-4 · Cited by 50
CRISPR‐Cas targeted plasmid integration into mammalian cells via non‐homologous end joining
Bachu, R., Bergareche, I., & Chasin, L. A. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25629 · Cited by 50
Key Applications of Plant Metabolic Engineering
Lau, W., Fischbach, M. A., Osbourn, A., & Sattely, E. S. (2014) · PLoS Biology · doi:10.1371/journal.pbio.1001879 · Cited by 50
CRISPR–Cas9 in genome editing: Its function and medical applications
Khadempar, S., Familghadakchi, S., Motlagh, R. A., Farahani, N., Dashtiahangar, M., Rezaei, H., & Hayat, S. M. G. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.27476 · Cited by 53
New and TALENted Genome Engineering Toolbox
Campbell, J. M., Hartjes, K. A., Nelson, T. J., Xu, X., & Ekker, S. C. (2013) · Circulation Research · doi:10.1161/circresaha.113.301765 · Cited by 48
“Eukaryotic cells repair the resulting double-strand breaks by either error-prone nonhomologous end joining (NHEJ), resulting in insertions or deletions (indels) at the cut site, or by homologous recombination (HR) or homologydirected repair (HDR) in the presence of an exogenous DNA donor (Figure 1).(13) If targeted to…”
Wang, Q., Chen, S., Xiao, Q., Liu, Z., Liu, S., Hou, P., Zhou, L., Hou, W., Ho, W., Li, C., et al. (2017) · Retrovirology · doi:10.1186/s12977-017-0375-0 · Cited by 49
“Over the last decade, novel genome-editing methods that utilize nucleases have been developed, including zinc finger nucleases (ZFNs) [11], transcription activator like-effector nucleases (TALENs) [12] and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated nuclease (Cas9) [13, 14].”
BackgroundEfficient engineered probiotics using synthetic biology approaches: A review
Yadav, M., & Shukla, P. (2019) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1822 · Cited by 49
Tailor‐made TALEN system for highly efficient targeted gene replacement in the rice blast fungus
Arazoe, T., Ogawa, T., Miyoshi, K., Yamato, T., Ohsato, S., Sakuma, T., Yamamoto, T., Arie, T., & Kuwata, S. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25559 · Cited by 49
Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing
Kim, D., Le, Q., Wu, Y., Park, J.-W., & Oh, Y. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121233 · Cited by 51
“Until the early 2010s, such genome engineering was mainly carried out using a specialized enzyme called zinc-finger nuclease (ZFN) and transcription activator-like effector nucleases (TALENs) [15,16].”
BackgroundGenetic correction using engineered nucleases for gene therapy applications
Li, H. L., Nakano, T., & Hotta, A. (2013) · Development Growth & Differentiation · doi:10.1111/dgd.12107 · Cited by 50
“Recent progress in gene editing technology using tailor-made nucleases, such as meganucleases (MNs), zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs) and, more recently, CRISPR/Cas9, has significantly broadened our ability to precisely modify target sites in the human genome.”
BackgroundInfluentialModeling Psychiatric Disorder Biology with Stem Cells
Das, D., Feuer, K. L., Wahbeh, M. H., & Avramopoulos, D. (2020) · Current Psychiatry Reports · doi:10.1007/s11920-020-01148-1 · Cited by 49
Integration of drug, protein, and gene delivery systems with regenerative medicine
Lorden, E. R., Levinson, H., & Leong, K. W. (2013) · Drug Delivery and Translational Research · doi:10.1007/s13346-013-0165-8 · Cited by 49
“Although in their infancy, TALENs have been suggested to cleave DNAwith similar efficiency as zinc finger nucleases, but boast simplistic design methods [47].”
Insights into maize genome editing via CRISPR/Cas9
Agarwal, A., Yadava, P., Kumar, K., Singh, I., Kaul, T., Pattanayak, A., & Agrawal, P. K. (2018) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-017-0502-3 · Cited by 48
Genome editing in large animal models
Maynard, L. H., Humbert, O., Peterson, C. W., & Kiem, H. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.09.026 · Cited by 50
Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants
Kaur, N., Sharma, S., Hasanuzzaman, M., & Pati, P. K. (2022) · International Journal of Genomics · doi:10.1155/2022/5547231 · Cited by 47
“Thus, TALEN also needs to dimerize to act on their target sites [47].”
BackgroundInfluentialA synthetic biology approach for the treatment of pollutants with microalgae
Webster, L. J., Gómez, D. K. V., Brown, R. B., Clarke, W. P., & Schenk, P. M. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1379301 · Cited by 47
“Naturally this bacterium injects a highly conserved repeat sequence into plant cells to alter transcription and allow for bacterial colonisation (Joung and Sander, 2013).”
BackgroundSynthetic Biology‐Based Engineering Cells for Drug Delivery
Yi, W., Hu, S., Qian, X., Yan, W., & Li, Y. (2025) · Exploration · doi:10.1002/exp.20240095 · Cited by 49
“Zinc-finger nucleases (ZFNs) [16], transcription activator-like (TAL) effector nucleases (TALENs) [17], and clustered regularly interspaced short palindromic repeat-associated 9 nucleases (CRISPR-Cas9) [18] are currently being developed for the construction and modulation of synthetic gene circuits…”
Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology
Mabashi‐Asazuma, H., Kuo, C., Khoo, K., & Jarvis, D. L. (2015) · ACS Chemical Biology · doi:10.1021/acschembio.5b00340 · Cited by 47
Progress towards the ‘Golden Age’ of biotechnology
Gartland, K. M. A., Bruschi, F., Dündar, M., Gahan, P. B., Magni, M. V., & Akbarova, Y. Y. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.05.011 · Cited by 47
“TALENs can be designed to target almost any DNA sequence and will have major impacts for the design and testing of therapeutic agents for specific genetic diseases [52,53].”
BackgroundStrauβ, A., & Lahaye, T. (2013) · Molecular Plant · doi:10.1093/mp/sst075 · Cited by 47
“Residues in position 13 that bind preferentially to adenine, cytosine, guanine, or thymine have been identified and this TALE-code facilitates the assembly of repeat arrays with any desired specificity (Joung and Sander, 2013).”
BackgroundInfluentialSato, M., Miyoshi, K., Nagao, Y., Nishi, Y., Ohtsuka, M., Nakamura, S., Sakurai, T., & Watanabe, S. (2014) · Xenotransplantation · doi:10.1111/xen.12089 · Cited by 47
“Recently, zinc-finger nucleases (ZFNs) and transcription activator-like effectors (TALENs) have emerged as powerful and efficient tools for gene editing [8].”
BackgroundRen, Y., Qiu, L., Lu, F.-L., Ru, X., Li, S., Xiang, Y., Yu, S., & Zhang, Y. (2016) · Scientific Reports · doi:10.1038/srep23775 · Cited by 45
Bastidas, R. J., & Valdivia, R. H. (2016) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00071-15 · Cited by 47
“In addition, an inducible expression system would be beneficial for epigenetic gene silencing approaches, such as the CRISPR/Cas9 system (166) and TALENS (167, 168), or for the expression of programmable repressors and dominant negative protein variants.”
BackgroundShock wave-induced permeabilization of mammalian cells
Lopez-Marin, L. M., Rivera, A. L., Fernández, F., & Loske, A. M. (2018) · Physics of Life Reviews · doi:10.1016/j.plrev.2018.03.001 · Cited by 49
Rai, P. K., Craven, L., Hoogewijs, K., Russell, O. M., & Lightowlers, R. N. (2018) · Essays in Biochemistry · doi:10.1042/ebc20170113 · Cited by 52
A Statistical Guide to the Design of Deep Mutational Scanning Experiments
Matuszewski, S., Hildebrandt, M. E., Ghenu, A., Jensen, J. D., & Bank, C. (2016) · Genetics · doi:10.1534/genetics.116.190462 · Cited by 49
An Engineered Cas-Transposon System for Programmable and Site-Directed DNA Transpositions
Chen, S. P., & Wang, H. H. (2019) · The CRISPR Journal · doi:10.1089/crispr.2019.0030 · Cited by 47
“4,5 Site-specific nucleases such as CRISPR-associated (Cas) nucleases, 6,7 zinc-finger nucleases (ZFNs), 8 and transcription activator-like effector nucleases (TALENs) 9 can be programmed to generate double-strand DNA breaks that are then repaired to incorporate a template DNA.”
Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression
Kabadi, A. M., & Gersbach, C. A. (2014) · Methods · doi:10.1016/j.ymeth.2014.06.014 · Cited by 50
“Because of their simple DNA recognition code, the frequency of engineering active TALE-based enzymes is very high (4, 39).”
BackgroundGenome editing strategies: potential tools for eradicating HIV-1/AIDS
Khalili, K., Kaminski, R., Gordon, J., Cosentino, L., & Hu, W. (2015) · Journal of NeuroVirology · doi:10.1007/s13365-014-0308-9 · Cited by 51
“In the past 3–4 years, TALEN has been extensively used in a large number of applications due to its easy design, high cleavage efficiency, and seemingly unlimited targeting range (Guilinger et al. 2014a; Joung and Sander 2013).”
BackgroundKnockout of ush2a gene in zebrafish causes hearing impairment and late onset rod-cone dystrophy
Han, S., Liu, X., Xie, S., Gao, M., Liu, F., Yu, S., Sun, P., Wang, C., Archacki, S. R., Lu, Z., et al. (2018) · Human Genetics · doi:10.1007/s00439-018-1936-6 · Cited by 46
Metabolic pathway engineering: Perspectives and applications
Dasgupta, A., Chowdhury, N., & De, R. K. (2020) · Computer Methods and Programs in Biomedicine · doi:10.1016/j.cmpb.2020.105436 · Cited by 47
Genome editing in zebrafish: a practical overview
Sertori, R., Trengove, M. C., Basheer, F., Ward, A. C., & Liongue, C. (2015) · Briefings in Functional Genomics · doi:10.1093/bfgp/elv051 · Cited by 44
Genome engineering for breaking barriers in lignocellulosic bioethanol production
Ulaganathan, K., Goud, S., Reddy, M., & Ulaganathan, K. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.01.028 · Cited by 45
Genome-edited livestock: Ethics and social acceptance
Ishii, T. (2017) · Animal Frontiers · doi:10.2527/af.2017.0115 · Cited by 51
“Genome editing tools, such as zincfinger nucleases (ZFNs; Klug, 2010), transcription activator-like effector nucleases (TALENs; Joung and Sander, 2013), and the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas 9 (Barrangou and Doudna, 2016), can break DNA double strands at target sites and then ac…”
BackgroundYong, L., Thet, Z., & Zhu, Y. (2017) · Journal of Experimental Biology · doi:10.1242/jeb.161596 · Cited by 45
“TALENs (transcription activator-like effector nucleases) and CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9), has opened opportunities to easily manipulate genes in unprecedented ways, affording the ability to test candidate genes linked to a behavior (Joung and Sand…”
BackgroundMining for missed sORF-encoded peptides
Yin, X., Jing, Y., & Xu, H. (2019) · Expert Review of Proteomics · doi:10.1080/14789450.2019.1571919 · Cited by 46
“Many gene editing techniques, such as CRISPR/Cas9 [112], TALENs [113] and ZFNs [114], can also be designed to validate sORF coding potential.”
BackgroundMitochondrial genome engineering coming-of-age
Barrera-Paez, J. D., & Moraes, C. T. (2022) · Trends in Genetics · doi:10.1016/j.tig.2022.04.011 · Cited by 44
Hillary, V. E., & Ceasar, S. A. (2022) · BioEssays · doi:10.1002/bies.202200032 · Cited by 45
“[3] Later, genome editing technologies such as meganucleases (MNs),[4] zinc finger nucleases (ZFNs),[5] and transcription activator-like effector nucleases (TALENs)[6] have been explored for a targeted knockout or knockdown studies to cleave double-stranded DNA (dsDNA) at a targeted region.”
BackgroundFarooqi, M. Q. U., Nawaz, G., Wani, S. H., Choudhary, J. R., Rana, M., Sah, R. P., Afzal, M., Zahra, Z., Ganie, S. A., Razzaq, A., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.965878 · Cited by 50
“Some of the wellknown genome editing tools are ZFNs (zinc-finger nucleases), TALENs (transcription activator-like effector nucleases), and clustered CRISPR (regularly interspaced short palindromic repeats)/Cas systems (Miller et al., 1985; Jinek et al., 2012; Joung and Sander, 2013).”
BackgroundZebrafish: A model vertebrate suitable for the analysis of human genetic disorders
Ota, S., & Kawahara, A. (2013) · Congenital Anomalies · doi:10.1111/cga.12040 · Cited by 44
Feng, W., Dai, Y., Mou, L., Cooper, D. K. C., Shi, D., & Cai, Z. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms16036545 · Cited by 44
Single-Stranded γPNAs for In Vivo Site-Specific Genome Editing via Watson-Crick Recognition
Bahal, R., Quijano, E., McNeer, N. A., Liu, Y., Bhunia, D. C., Lopez-Giraldez, F., Fields, R. J., Saltzman, W. M., Ly, D. H., & Glazer, P. M. (2014) · Current Gene Therapy · doi:10.2174/1566523214666140825154158 · Cited by 45
“One approach involves the use of engineered nucleases, including Meganucleases [1, 2], zinc-finger nucleases (ZFNs) [3, 4], TAL effector nucleases (TALENs) [5, 6], the CRISPR/Cas9 system [7, 8] and artificial DNA cutters [9].”
BackgroundThe role of chromatin and epigenetics in the polyphenisms of ant castes
Bonasio, R. (2014) · Briefings in Functional Genomics · doi:10.1093/bfgp/elt056 · Cited by 40
“editing technologies [77, 78] have lifted some of”
Beyond the Mouse Monopoly: Studying the Male Germ Line in Domestic Animal Models
González, R., & Dobrinski, I. (2015) · ILAR Journal · doi:10.1093/ilar/ilv004 · Cited by 43
Ji, Y., Buel, S. M., & Amack, J. D. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.06.010 · Cited by 43
“Abnormalities associated with pituitary defects are found in ARS patients (Chang et al., 2012; Tumer and Bach-Holm, 2009) and pitx2 knockout mice (Gage et al., 1999; Kitamura et al., 1999; Lin et al., 1999; Lu et al., 1999; Suh et al., 2002).”
InfluentialKey Players in the Mutant p53 Team: Small Molecules, Gene Editing, Immunotherapy
Chasov, V. V., Mirgayazova, R., Zmievskaya, E., Khadiullina, R., Valiullina, A., Clarke, J. R. S., Rizvanov, A. A., Baud, M. G. J., & Bulatov, E. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.01460 · Cited by 42
“CRISPR/Cas Gene Editing There are numerous molecular tools for programmable genome editing at a clinical level, including zinc-finger nucleases (ZFNs) (49, 50), transcription activator-like effector nucleases (TALENs) (51, 52), clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)…”
BackgroundSolin, S. L., Shive, H. R., Woolard, K., Essner, J. J., & McGrail, M. A. (2015) · Scientific Reports · doi:10.1038/srep13745 · Cited by 41
Genome engineering: Drosophila melanogaster and beyond
Venken, K. J. T., Sarrion‐Perdigones, A., Vandeventer, P. J., Abel, N. S., Christiansen, A. E., & Hoffman, K. L. (2015) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.214 · Cited by 43
“These RVD-nucleotide associations are not exclusive, and most pairs can incorporate mismatches.(135) Nevertheless, multiple TALE repeats can be serially linked to form a synthetic DNA-binding protein that recognizes up to two dozen nucleotides with high specificity(135) (Figure 4(c)).”
BackgroundInfluentialWang, Y., Wang, D., Wang, X., Tao, H., Feng, E., Zhu, L., Pan, C., Wang, B., Liu, C., Liu, X., et al. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01932 · Cited by 43
Present and Future Therapies for Chronic Hepatitis B
Tao, Y.-C., Wu, D., Zhou, L., Chen, E., Liu, C., Tang, X., Jiang, W., Han, N., Li, H., & Tang, H. (2019) · Advances in experimental medicine and biology · doi:10.1007/978-981-13-9151-4_6 · Cited by 42
Cho, H., Lee, K.-H., Shen, Y., Shin, T.-J., Ryu, P. D., Choi, M., Kang, K., & Cho, J. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2020.01.007 · Cited by 42
Omics Approach to Axonal Dysfunction of Motor Neurons in Amyotrophic Lateral Sclerosis (ALS)
Suzuki, N., Akiyama, T., Warita, H., & Aoki, M. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00194 · Cited by 46
“…the physiological and pathological features of neuronal axons, including cargo transport within axons, local translation, and the axonal transcriptome (Jung et al., 2012; Maday et al., 2014; Batista and Hengst, 2016; Neto et al., 2016; Brady and Morfini, 2017; De Vos and Hafezparast, 2017).”
BackgroundFDA orphan drug designations for lysosomal storage disorders – a cross-sectional analysis
Garbade, S. F., Zielonka, M., Mechler, K., Kölker, S., Hoffmann, G. F., Staufner, C., Mengel, E., & Ries, M. (2020) · PLoS ONE · doi:10.1371/journal.pone.0230898 · Cited by 41
“Those approaches are currently limited to applications in basic research, but transfer into clinical trials can be expected in the near future [34, 35].”
BackgroundHayashi, T., Sakamoto, K., Sakuma, T., Yokotani, N., Inoue, T., Kawaguchi, E., Agata, K., Yamamoto, T., & Takeuchi, T. (2013) · Development Growth & Differentiation · doi:10.1111/dgd.12103 · Cited by 41
“…zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and the RNAguided CRISPR/Cas9 nuculease system have enabled targeted alteration of any genomic sequence in a wide range of organisms (Urnov et al. 2010; Daboussi et al. 2012; Joung & Sander 2013; Wei et al. 2013).”
BackgroundConditional targeted genome editing using somatically expressed TALENs in C. elegans
Cheng, Z., Yi, P., Wang, X., Chai, Y., Feng, G., Yang, Y., Liang, X., Zhu, Z., Li, W., & Ou, G. (2013) · Nature Biotechnology · doi:10.1038/nbt.2674 · Cited by 41
O’Brien, S. A., Lee, K., Fu, H., Lee, Z., Le, T. S., Stach, C. S., McCann, M. G., Zhang, A. Q., Smanski, M. J., Somia, N. V., et al. (2018) · Biotechnology Journal · doi:10.1002/biot.201800226 · Cited by 41
Stein, H. P., Navajas‐Pérez, R., & Aranda, E. (2018) · Nanotechnology in the life sciences · doi:10.1007/978-3-030-02369-0_4 · Cited by 41
Purification and enrichment of specific chromatin loci
Gauchier, M., van Mierlo, G., Vermeulen, M., & Déjardin, J. (2020) · Nature Methods · doi:10.1038/s41592-020-0765-4 · Cited by 41
Current Approaches and Applications in Avian Genome Editing
Lee, J., Kim, D., & Lee, K. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21113937 · Cited by 42
“To improve the efficiency and accuracy of conventional gene targeting, a new method relying on site-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), was developed [2,3].”
MethodInduced mutagenesis for genetic improvement of Allium genetic resources: a comprehensive review
Singh, H., Khar, A., & Verma, P. (2021) · Genetic Resources and Crop Evolution · doi:10.1007/s10722-021-01210-8 · Cited by 41
The Development of TALE Nucleases for Biotechnology
Ousterout, D. G., & Gersbach, C. A. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2932-0_3 · Cited by 42
“There are numerous platforms for generating site-specific gene modifications in the genome, but to date the most successful have been based on zinc finger nucleases (3,4), TALENs (5,6) and more recently, the RNA-guided CRISPR/Cas9 system (7–9).”
BackgroundCRISPR-Cas9: from Genome Editing to Cancer Research
Chen, S., Sun, H., Miao, K., & Deng, C. (2016) · International Journal of Biological Sciences · doi:10.7150/ijbs.17421 · Cited by 40
“By ranging the modules in different ways, the enzyme could target specific sequences with relatively high frequency [17-19].”
BackgroundChickpea (Cicer arietinum L.) Cytogenetics, Genetic Diversity and Breeding
Raina, A., Khan, S., Wani, M. R., Laskar, R. A., & Mushtaq, W. (2019) · Springer eBooks · doi:10.1007/978-3-030-23400-3_3 · Cited by 42
Endo, T., Fujii, H., Omura, M., & Shimada, T. (2020) · BMC Plant Biology · doi:10.1186/s12870-020-02399-z · Cited by 40
Mammalian Germ Cell Development: From Mechanism to In Vitro Reconstitution
Saitou, M. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.01.008 · Cited by 42
A most formidable arsenal: genetic technologies for building a better mousePreprint
Clark, J. F., Dinsmore, C. J., & Soriano, P. M. (2020) · Genes & Development · doi:10.1101/gad.342089.120 · Cited by 40
Shen, J.-Y., Zhao, Q., & He, Q. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00099 · Cited by 39
Soybean Oil and Protein: Biosynthesis, Regulation and Strategies for Genetic Improvement
Li, H., Sun, J., Zhang, Y., Wang, N., Li, T., Dong, H., Yang, M., Xu, C. J., Hu, L., Liu, C., et al. (2024) · Plant Cell & Environment · doi:10.1111/pce.15272 · Cited by 39
“Tools derived from the CRISPR/Cas9 system, such as base editors and master editors, can expand the scope of genome editing and increase the efficiency of editing tools (Bai et al. 2024).”
Progress and Prospects of Anti-HBV Gene Therapy Development
Maepa, M. B., Roelofse, I., Ely, A., & Arbuthnot, P. B. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms160817589 · Cited by 41
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR /Cas9
Abbasi, F., Miyata, H., & Ikawa, M. (2017) · Reproductive Medicine and Biology · doi:10.1002/rmb2.12067 · Cited by 38
Okano, H., & Kishi, N. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2017.10.016 · Cited by 40
CRISPR/Cas9 disease models in zebrafish and Xenopus: The genetic renaissance of fish and frogs
Naert, T., & Vleminckx, K. (2018) · Drug Discovery Today Technologies · doi:10.1016/j.ddtec.2018.07.001 · Cited by 42
Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis
Hong, K., Liu, D., Chen, T., & Wang, Z. (2018) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-018-2537-1 · Cited by 39
Common therapeutic advances for Duchenne muscular dystrophy (DMD)
Salmaninejad, A., Abarghan, Y. J., Qomi, S. B., Bayat, H., Yousefi, M., Azhdari, S., Talebi, S., & Mojarrad, M. (2020) · International Journal of Neuroscience · doi:10.1080/00207454.2020.1740218 · Cited by 41
Kabir, S. M. T., Hossain, M. S., Bashar, K. K., Honi, U., Ahmed, B., Emdad, E. M., Alam, M. M., Haque, M. S., & Islam, M. S. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.113469 · Cited by 39
CRISPR‐Cas9 ‐mediated editing of BADH2 gene triggered fragrance revolution in rice
Imran, M., Shafiq, S., & Tang, X. (2023) · Physiologia Plantarum · doi:10.1111/ppl.13871 · Cited by 39
Next generation mothers: Maternal control of germline development in zebrafish
Dosch, R. (2014) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2014.985816 · Cited by 45
Myoediting: Toward Prevention of Muscular Dystrophy by Therapeutic Genome Editing
Zhang, Y., Long, C., Bassel‐Duby, R., & Olson, E. N. (2018) · Physiological Reviews · doi:10.1152/physrev.00046.2017 · Cited by 42
“TALENs have been widely used to target genomes of various species including cultured cells, animals, and plants (116, 170, 352, 380).”
BackgroundThe Mechanisms of Genome Editing Technologies in Crop Plants
Ahmad, Y., Haider, S., Iqbal, J., Abbasi, B. A., Yaseen, T., & Mahmood, T. (2022) · Springer eBooks · doi:10.1007/978-3-030-96925-7_13 · Cited by 40
Ahmad, A., Mustafa, G., Rana, A. Z., & Zia, A. R. (2023) · Current Microbiology · doi:10.1007/s00284-023-03316-x · Cited by 38
“These genome editing tools belong to the nonspecific class of type 2 restriction enzymes and are synthesized by fusing the transcription activator-like effectors (TALEs) binding domain with DNA cleavage domain with FoK1 nuclease activity [51, 53].”
BackgroundOverview of guide RNA design tools for CRISPR-Cas9 genome editing technology
Zhu, L. J. (2015) · Frontiers in Biology · doi:10.1007/s11515-015-1366-y · Cited by 38
da Cunha, N. B., Vianna, G. R., Lima, T. D. A., & Rech, E. L. (2013) · Biotechnology Journal · doi:10.1002/biot.201300062 · Cited by 38
A Model Study of In Silico Proficiency Testing for Clinical Next-Generation Sequencing
Duncavage, E. J., Abel, H., Merker, J. D., Bodner, J. B., Zhao, Q., Voelkerding, K. V., & Pfeifer, J. D. (2016) · Archives of Pathology & Laboratory Medicine · doi:10.5858/arpa.2016-0194-cp · Cited by 38
Enzyme therapy: a forerunner in catalyzing a healthy society?
Datta, S., Rajnish, K. N., Doss, C. G. P., Samuel, S. M., Ethiraj, S., & Zayed, H. (2020) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2020.1787980 · Cited by 38
Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects
Bhatta, B. P., & Malla, S. (2020) · Plants · doi:10.3390/plants9101360 · Cited by 40
“Many studies have utilized these tools: mega nucleases in maize (Zea mays) [45], ZFNs in maize, Arabidopsis, and soybean (Glycine max) [46–48], and TALENs in rice (Oryza sativa) [49,50], wheat (Triticum aestivum) [14], maize [51], tomato (Solanum lycopersicum) [52], and Arabidopsis [53].”
CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering
Saifaldeen, M., Al-Ansari, D. E., Ramotar, D., & Aouida, M. (2020) · Cells · doi:10.3390/cells9112518 · Cited by 40
“(~3 Kb compared to ~1 Kb for ZFNs) limits its packaging and delivery [13,39].”
BackgroundXiao, H., Hu, J., Huang, C., Feng, W., Liu, Y., Kumblathan, T., Tao, J., Xu, J., Le, X. C., & Zhang, H. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117000 · Cited by 39
“Thus, the CRISPR-Cas system is simpler and more efficient than other genome-editing nucleases, such as meganucleases [11], zinc-finger nucleases (ZFNs) [12], and transcription activator-like effector nucleases (TALENs) [13].”
Application of CRISPR/Cas9 System in Establishing Large Animal Models
Lin, Y., Li, J., Li, C., Tu, Z., Li, S., Li, X., & Yan, S. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.919155 · Cited by 42
Genetic biofortification: advancing crop nutrition to tackle hidden hunger
Zulfiqar, U., Khokhar, A., Maqsood, M. F., Shahbaz, M., Naz, N., Sara, M., Maqsood, S., Sahar, S., Hussain, S., & Ahmad, M. (2024) · Functional & Integrative Genomics · doi:10.1007/s10142-024-01308-z · Cited by 37
“TALENs are engineered with a TAL effector binding DNA domain attached to the FokI nuclease (Joung and Sander 2013).”
Staurosporine-Derived Inhibitors Broaden the Scope of Analog-Sensitive Kinase Technology
Lopez, M. S., Choy, J. W., Peters, U., Sos, M. L., Morgan, D. O., & Shokat, K. M. (2013) · Journal of the American Chemical Society · doi:10.1021/ja408704u · Cited by 37
Ishizaki, T. (2016) · Molecular Breeding · doi:10.1007/s11032-016-0591-7 · Cited by 37
“Genome editing is a new approach that can induce mutations at specific loci in the genomes of cellular organisms (Gaj et al. 2013; Hsu et al. 2014; Joung and Sander 2013).”
Background
Metadata
- Authors
- Joung, J. K., & Sander, J. D.
- Affiliations
- Harvard University (US)
- Corresponding author
- Joung, J. Keith
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2012-11-21
- Volume
- 14
- Issue
- 1
- Pages
- 49-55
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- PMC
- PMC3547402
- OpenAlex
- W2061184966
- Cited by
- 1,650
- References
- 103
- Citations by year
- 2026: 65 · 2025: 106 · 2024: 128 · 2023: 119 · 2022: 123 · 2021: 102 · 2020: 112 · 2019: 122 · 2018: 114 · 2017: 103 · 2016: 114 · 2015: 159 · 2014: 175 · 2013: 107
- FWCI
- 12.87
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Human Genome Research Institute (P50HG005550)
- National Cancer Institute (T32CA009216)
- National Institute of General Medical Sciences (DP1GM105378, R01GM088040)
- National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- TALEN, targeted genome editing, engineered nucleases, genetic modification, genetic disease treatment
- MeSH terms
- Animals, Deoxyribonucleases, DNA, DNA-Binding Proteins, Humans, Protein Engineering, Transcriptional Activation, Genome, Protein Structure, Tertiary
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