How retroviruses select their genomes
Abstract
As retroviruses assemble in infected cells, two copies of their full-length, unspliced RNA genomes are selected for packaging from a cellular milieu that contains a substantial excess of non-viral and spliced viral RNAs. Understanding the molecular details of genome packaging is important for the development of new antiviral strategies and to enhance the efficacy of retroviral vectors used in human gene therapy. Recent studies of viral RNA structure in vitro and in vivo and high-resolution studies of RNA fragments and protein-RNA complexes are helping to unravel the mechanism of genome packaging and providing the first glimpses of the initial stages of retrovirus assembly.
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Retroviral genome packaging takes place with great specificity despite the RNA being present in low abundance in infected cells compared to host and viral subgenomic RNAs.
“This takes place with great specificity despite being present in low abundance in the infected cells in comparison to host and viral subgenomic RNAs”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as D’Souza and Summers 2005
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- Authors
- D'Souza, V., & Summers, M. F.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Summers, Michael F.
- ORCID
- Summers, Michael F.
- Venue
- Nature Reviews Microbiology
- Published
- 2005-08
- Volume
- 3
- Issue
- 8
- Pages
- 643-655
- ISSN
- 1740-1526 (ISSN-L)
- 1740-1534
- OpenAlex
- W2016719543
- Cited by
- 339
- References
- 166
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 8 · 2023: 11 · 2022: 8 · 2021: 17 · 2020: 15 · 2019: 12 · 2018: 17 · 2017: 13 · 2016: 21 · 2015: 19 · 2014: 18 · 2013: 23 · 2012: 19
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Keywords
- retroviruses, genome packaging, viral RNA, RNA splicing, viral RNA structure, retroviral assembly, protein-RNA complex, antiviral strategies, retroviral vectors, gene therapy
- MeSH terms
- Animals, Base Sequence, Humans, Molecular Sequence Data, Moloney murine leukemia virus, Nucleic Acid Conformation, Retroviridae, RNA, Viral, HIV-1, Gene Products, gag, Genome, Viral, Virus Assembly
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