Structure of the DBL3x domain of pregnancy-associated malaria protein VAR2CSA complexed with chondroitin sulfate A
Open accessAbstract
Plasmodium falciparum-infected erythrocytes bind to chondroitin sulfate A (CSA) in the placenta via the VAR2CSA protein, a member of the P. falciparum erythrocyte membrane protein-1 family, leading to life-threatening malaria in pregnant women with severe effects on their fetuses and newborns. Here we describe the structure of the CSA binding DBL3x domain, a Duffy binding-like (DBL) domain of VAR2CSA. By forming a complex of DBL3x with CSA oligosaccharides and determining its structure, we have identified the CSA binding site to be a cluster of conserved positively charged residues on subdomain 2 and subdomain 3. Mutation or chemical modification of lysine residues at the site markedly diminished CSA binding to DBL3x. The location of the CSA binding site is an important step forward in the molecular understanding of pregnancy-associated malaria and offers a new target for vaccine development.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
[20] Processing of X-ray diffraction data collected in oscillation mode
Otwinowski, Z., & Minor, W. (1997) · Methods in Enzymology · doi:10.1016/s0076-6879(97)76066-x · Cited by 33,475 · see citing works
<i>Phaser</i> crystallographic software
McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., & Read, R. J. (2007) · Journal of Applied Crystallography · doi:10.1107/s0021889807021206 · Cited by 20,612 · see citing works
The CCP4 suite: programs for protein crystallography
Number Collaborative Computational Project (1994) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444994003112 · Cited by 17,299 · see citing works
Kabsch, W. (1993) · Journal of Applied Crystallography · doi:10.1107/s0021889893005588 · Cited by 3,562 · see citing works
Version 1.2 of the Crystallography and NMR system
Brünger, A. T. (2007) · Nature Protocols · doi:10.1038/nprot.2007.406 · Cited by 1,325 · see citing works
Su, X.-Z., Heatwole, V. M., Wertheimer, S. P., Guinet, F., Herrfeldt, J. A., Peterson, D. S., Ravetch, J. A., & Wellems, T. E. (1995) · Cell · doi:10.1016/0092-8674(95)90055-1 · Cited by 1,219 · see citing works
Adherence of <i>Plasmodium falciparum</i> to Chondroitin Sulfate A in the Human Placenta
Fried, M., & Duffy, P. E. (1996) · Science · doi:10.1126/science.272.5267.1502 · Cited by 1,108 · see citing works
Baruch, D., Pasloske, B., Singh, H. B., Bi, X., Ma, X. C., Feldman, M., Taraschi, T., & Howard, R. J. (1995) · Cell · doi:10.1016/0092-8674(95)90054-3 · Cited by 1,087 · see citing works
Smith, J. D., Chitnis, C. E., Craig, A. G., Roberts, D. J., Hudson-Taylor, D. E., Peterson, D. S., Pinches, R., Newbold, C. I., & Miller, L. H. (1995) · Cell · doi:10.1016/0092-8674(95)90056-x · Cited by 1,005 · see citing works
Self-citationSalanti, A., Staalsoe, T., Lavstsen, T., Jensen, A. T. R., Sowa, M. P. K., Arnot, D. E., Hviid, L., & Theander, T. G. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03570.x · Cited by 702 · see citing works
Maternal antibodies block malaria
Fried, M., Nosten, F., Brockman, A., Brabin, B. J., & Duffy, P. E. (1998) · Nature · doi:10.1038/27570 · Cited by 659 · see citing works
The Sick Placenta—The Role of Malaria
Brabin, B. J., Romagosa, C., Abdelgalil, S., Menéndez, C., Verhoeff, F. H., McGready, R. M., Fletcher, K. A., Owens, S., d'Alessandro, U., Nosten, F. H., et al. (2004) · Placenta · doi:10.1016/j.placenta.2003.10.019 · Cited by 374 · see citing works
Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes.
Rogerson, S. J., Chaiyaroj, S. C., Ng, K., Reeder, J. C., & Brown, G. V. (1995) · The Journal of Experimental Medicine · doi:10.1084/jem.182.1.15 · Cited by 342 · see citing works
Tolia, N. H., Enemark, E. J., Sim, B. K. L., & Joshua‐Tor, L. (2005) · Cell · doi:10.1016/j.cell.2005.05.033 · Cited by 331 · see citing works
Structural basis for Duffy recognition by the malaria parasite Duffy-binding-like domain
Singh, S. K., Hora, R., Belrhali, H., Chitnis, C. E., & Sharma, A. (2006) · Nature · doi:10.1038/nature04443 · Cited by 247 · see citing works
Viebig, N. K., Gamain, B., Scheidig, C., Lépolard, C., Przyborski, J., Lanzer, M., Gysin, J., & Scherf, A. (2005) · The EMBO Reports · doi:10.1038/sj.embor.7400466 · Cited by 201 · see citing works
Achur, R. N., Valiyaveettil, M., Alkhalil, A., Ockenhouse, C. F., & Gowda, D. C. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m006398200 · Cited by 162 · see citing works
Self-citationGamain, B., Trimnell, A. R., Scheidig, C., Scherf, A., Miller, L. H., & Smith, J. D. (2005) · The Journal of Infectious Diseases · doi:10.1086/428137 · Cited by 145 · see citing works
Self-citationReductive alkylation of lysine residues to alter crystallization properties of proteins.
Rayment, I. (1997) · PubMed · Cited by 141 · see citing works
[12] Reductive alkylation of lysine residues to alter crystallization properties of proteins
Rayment, I. (1997) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(97)76058-0 · Cited by 131 · see citing works
Bockhorst, J., Lu, F., Janes, J. H., Keebler, J., Gamain, B., Awadalla, P., Su, X.-Z., Samudrala, R., Jojic, N., & Smith, J. D. (2007) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2007.06.007 · Cited by 117 · see citing works
Duffy, M. F., Maier, A. G., Byrne, T. J., Marty, A. J., Elliott, S. R., O’Neill, M. T., Payne, P. D., Rogerson, S. J., Cowman, A. F., Crabb, B. S., et al. (2006) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2006.03.006 · Cited by 114 · see citing works
Alkhalil, A., Achur, R. N., Valiyaveettil, M., Ockenhouse, C. F., & Gowda, D. C. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m006399200 · Cited by 109 · see citing works
Self-citationFried, M., Domingo, G. J., Gowda, C. D., Mutabingwa, T. K., & Duffy, P. E. (2006) · Experimental Parasitology · doi:10.1016/j.exppara.2005.12.003 · Cited by 107 · see citing works
The Structure of a Chondroitin Sulfate-binding Domain Important in Placental Malaria
Higgins, M. K. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.c800086200 · Cited by 99 · see citing works
Dahlbäck, M., Rask, T. S., Andersen, P. H., Nielsen, M. A., Ndam, N. T., Resende, M., Turner, L., Deloron, P., Hviid, L., Lund, O., et al. (2006) · PLoS Pathogens · doi:10.1371/journal.ppat.0020124 · Cited by 98 · see citing works
The Immunology and Pathogenesis of Malaria During Pregnancy
Beeson, J. G., & Duffy, P. E. (2005) · Current topics in microbiology and immunology · doi:10.1007/3-540-29967-x_6 · Cited by 94 · see citing works
Chondroitin 4-sulfate: The structure of a sulfated glycosaminoglycan
Winter, W. T., Arnott, S., Isaac, D. H., & Atkins, E. D. T. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90251-6 · Cited by 91 · see citing works
Tan, K., Duquette, M. A., Liu, J., Zhang, R., Joachimiak, A., Wang, J., & Lawler, J. (2006) · Structure · doi:10.1016/j.str.2005.09.017 · Cited by 87 · see citing works
Interactive electron-density map interpretation: fromINTERtoO
Jones, T. A. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904023509 · Cited by 72 · see citing works
VAR2CSA and protective immunity against pregnancy-associatedPlasmodium falciparummalaria
Hviid, L., & Salanti, A. (2007) · Parasitology · doi:10.1017/s0031182007000121 · Cited by 69 · see citing works
Sodeoka, M., Larson, C. J., Chen, L., LeClair, K. P., & Verdine, G. L. (1993) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/s0960-894x(00)80293-0 · Cited by 65 · see citing works
Characterization of anti-var2CSA-PfEMP1 cytoadhesion inhibitory mouse monoclonal antibodies
Avril, M., Gamain, B., Lépolard, C., Viaud, N., Scherf, A., & Gysin, J. (2006) · Microbes and Infection · doi:10.1016/j.micinf.2006.09.005 · Cited by 61 · see citing works
The distinct proteome of placental malaria parasites
Fried, M., Hixson, K. K., Anderson, L., Ogata, Y., Mutabingwa, T. K., & Duffy, P. E. (2007) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2007.05.010 · Cited by 61 · see citing works
The surface variant antigens of Plasmodium falciparum contain cross-reactive epitopes
Gamain, B., Miller, L. H., & Baruch, D. I. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.041602598 · Cited by 50 · see citing works
Self-citationPregnancy-associated malaria: Parasite binding, natural immunity and vaccine development
Gamain, B., Smith, J. D., Viebig, N. K., Gysin, J., & Scherf, A. (2006) · International Journal for Parasitology · doi:10.1016/j.ijpara.2006.11.011 · Cited by 49 · see citing works
Gamain, B., Smith, J. D., Avril, M., Baruch, D. I., Scherf, A., Gysin, J., & Miller, L. H. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04145.x · Cited by 40 · see citing works
Self-citationHeparin-induced cis- and trans-Dimerization Modes of the Thrombospondin-1 N-terminal Domain
Tan, K., Duquette, M. A., Liu, J., Shanmugasundaram, K., Joachimiak, A., Gallagher, J. T., Rigby, A. C., Wang, J., & Lawler, J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m705203200 · Cited by 34 · see citing works
Muthusamy, A. (2004) · Glycobiology · doi:10.1093/glycob/cwh077 · Cited by 25 · see citing works
Saxena, A. K., Saul, A. J., & Garboczi, D. N. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904001398 · Cited by 8 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
The repertoire of glycan determinants in the human glycome
Cummings, R. D. (2009) · Molecular BioSystems · doi:10.1039/b907931a · Cited by 487 · see citing works
Variant surface antigens of Plasmodium falciparum and their roles in severe malaria
Wahlgren, M., Goel, S., & Akhouri, R. R. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.47 · Cited by 237 · see citing works
New insights into malaria pathogenesis
Moxon, C. A., Gibbins, M. P., McGuinness, D., Milner, D. A., & Marti, M. (2020) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathmechdis-012419-032640 · Cited by 207 · see citing works
Glycosaminoglycans in infectious disease
Kamhi, E., Joo, E. J., Dordick, J. S., & Linhardt, R. J. (2013) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12034 · Cited by 192 · see citing works
Clausen, T. M., Christoffersen, S., Dahlbäck, M., Langkilde, A. E., Jensen, K. E., Resende, M., Agerbæk, M. Ø., Andersen, D., Berisha, B., Ditlev, S. B., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.348839 · Cited by 173 · see citing works
(2015) · Advances in Parasitology · doi:10.1016/bs.apar.2015.02.004 · Cited by 162 · see citing works
Srivastava, A., Gangnard, S., Round, A., Dechavanne, S., Juillerat, A., Raynal, B., Faure, G., Baron, B., Ramboarina, S., Singh, S. K., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000951107 · Cited by 149 · see citing works
Structural Basis for the ABO Blood-Group Dependence of Plasmodium falciparum Rosetting
Vigan-Womas, I., Guillotte-Blisnick, M., Juillerat, A., Hessel, A., Raynal, B. D. E., England, P., Cohen, J. H. M., Bertrand, O., Peyrard, T., Bentley, G. A., et al. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002781 · Cited by 100 · see citing works
Designing a VAR2CSA-based vaccine to prevent placental malaria
Fried, M., & Duffy, P. E. (2015) · Vaccine · doi:10.1016/j.vaccine.2015.10.011 · Cited by 86 · see citing works
“Following these observations, two crystallography studies using recombinant DBL3 protein of the A4 allele confirmed binding to CSA [19,20].”
BackgroundEndemic Burkitt's lymphoma as a polymicrobial disease
Chêne, A., Donati, D., Orem, J., Björkman, A., Mbidde, E. R., Kironde, F., Wahlgren, M., & Bejarano, M. T. (2009) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2009.10.002 · Cited by 89 · see citing works
The Chondroitin Sulfate A-binding Site of the VAR2CSA Protein Involves Multiple N-terminal Domains
Dahlbäck, M., Jørgensen, L. M., Nielsen, M. A., Clausen, T. M., Ditlev, S. B., Resende, M., Pinto, V. V., Arnot, D. E., Theander, T. G., & Salanti, A. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.191510 · Cited by 85 · see citing works
Patarroyo, M. E., Bermúdez, A., & Patarroyo, M. A. (2011) · Chemical Reviews · doi:10.1021/cr100223m · Cited by 84 · see citing works
Structural basis for placental malaria mediated by Plasmodium falciparum VAR2CSA.
Ma, R., Lian, T., Huang, R., Renn, J. P., Petersen, J. D., Zimmerberg, J., Duffy, P. E., & Tolia, N. H. (2021) · Nature Microbiology · doi:10.1038/s41564-020-00858-9 · Cited by 72 · see citing works
Singh, K., Gittis, A. G., Gitti, R. K., Ostazeski, S. A., Su, H.-P., & Garboczi, D. N. (2016) · Journal of Virology · doi:10.1128/jvi.02933-15 · Cited by 72 · see citing works
Self-citationModulation of Aβ 42 fìbrillogenesis by glycosaminoglycan structure
Valle‐Delgado, J. J., Alfonso‐Prieto, M., de Groot, N. S., Ventura, S., Samitier, J., Rovira, C., & Fernàndez‐Busquets, X. (2010) · The FASEB Journal · doi:10.1096/fj.09-153551 · Cited by 70 · see citing works
Plasmodium vivax Adherence to Placental Glycosaminoglycans
Chotivanich, K., Udomsangpetch, R., Suwanarusk, R., Pukrittayakamee, S., Wilairatana, P., Beeson, J. G., Day, N. P. J., & White, N. J. (2012) · PLoS ONE · doi:10.1371/journal.pone.0034509 · Cited by 88 · see citing works
Tutterrow, Y. L., Avril, M., Singh, K., Long, C. A., LEKE, R. J. I., Sama, G., Salanti, A., Smith, J. D., Leke, R. G. F., & Taylor, D. W. (2012) · Infection and Immunity · doi:10.1128/iai.00071-12 · Cited by 65 · see citing works
Self-citationMalpede, B. M., Lin, D. H., & Tolia, N. H. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.450643 · Cited by 63 · see citing works
Nanotools for the Delivery of Antimicrobial Peptides
Urban, P., Valle‐Delgado, J. J., Moles, E., Marques, J., Diez, C., & Fernàndez‐Busquets, X. (2012) · Current Drug Targets · doi:10.2174/138945012802002302 · Cited by 61 · see citing works
Khunrae, P., Philip, J. M. D., Bull, D. R., & Higgins, M. K. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2009.08.027 · Cited by 61 · see citing works
Juillerat, A., Lewit‐Bentley, A., Guillotte-Blisnick, M., Gangnard, S., Hessel, A., Baron, B., Vigan-Womas, I., England, P., Mercereau‐Puijalon, O., & Bentley, G. A. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1018692108 · Cited by 59 · see citing works
Hommel, M., Elliott, S. R., Soma, V., Kelly, G., Fowkes, F. J. I., Chesson, J. M., Duffy, M. F., Bockhorst, J. P., Avril, M., Müeller, I., et al. (2010) · Infection and Immunity · doi:10.1128/iai.01365-09 · Cited by 56 · see citing works
Zhang, B., & Chi, L. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.693563 · Cited by 50 · see citing works
Resende, M., Ditlev, S. B., Nielsen, M. A., Bodevin, S., Bruun, S., Pinto, V. V., Clausen, H., Turner, L., Theander, T. G., Salanti, A., et al. (2009) · International Journal for Parasitology · doi:10.1016/j.ijpara.2009.02.022 · Cited by 48 · see citing works
Chen, E., Salinas, N. D., Ntumngia, F. B., Adams, J. H., & Tolia, N. H. (2015) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0003644 · Cited by 46 · see citing works
Rieger, H., Yoshikawa, H., Quadt, K. A., Nielsen, M. A., Sanchez, C. P., Salanti, A., Tanaka, M., & Lanzer, M. (2014) · Blood · doi:10.1182/blood-2014-03-561019 · Cited by 43 · see citing works
Brown, A., Turner, L., Christoffersen, S., Andrews, K. A., Szestak, T., Zhao, Y., Larsen, S., Craig, A. G., & Higgins, M. K. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.416347 · Cited by 43 · see citing works
Avril, M., Hathaway, M. J., Srivastava, A., Dechavanne, S., Hommel, M., Beeson, J. G., Smith, J. D., & Gamain, B. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016622 · Cited by 40 · see citing works
IgM, FcμRs, and Malarial Immune Evasion
Czajkowsky, D. M., Salanti, A., Ditlev, S. B., Shao, Z., Ghumra, A., Rowe, J. A., & Pleass, R. J. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.1000203 · Cited by 43 · see citing works
Valle‐Delgado, J. J., Urbán, P., & Fernàndez‐Busquets, X. (2012) · Nanoscale · doi:10.1039/c2nr32821f · Cited by 41 · see citing works
Oriero, E. C., Amenga-Etego, L. N.-K., Ishengoma, D. S., & Amambua‐Ngwa, A. (2021) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2020.1838440 · Cited by 39 · see citing works
Progress and Insights Toward an Effective Placental Malaria Vaccine
Gamain, B., Chêne, A., Viebig, N. K., Ndam, N. T., & Nielsen, M. A. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.634508 · Cited by 40 · see citing works
Pomin, V. H., Park, Y., Huang, R., Heiß, C., Sharp, J. S., Azadi, P., & Prestegard, J. H. (2012) · Glycobiology · doi:10.1093/glycob/cws055 · Cited by 40 · see citing works
Urban, P., & Fernandez-Busquets, X. (2014) · Current Medicinal Chemistry · doi:10.2174/09298673113206660292 · Cited by 39 · see citing works
Avril, M., Cartwright, M. M., Hathaway, M. J., Hommel, M., Elliott, S. R., Williamson, K., Narum, D. L., Duffy, P. E., Fried, M., Beeson, J. G., et al. (2010) · Infection and Immunity · doi:10.1128/iai.00410-09 · Cited by 39 · see citing works
The case for PfEMP1-based vaccines to protect pregnant women againstPlasmodium falciparummalaria
Hviid, L. (2011) · Expert Review of Vaccines · doi:10.1586/erv.11.113 · Cited by 35 · see citing works
Macchione, G., Maza, S., Kayser, M. M., de Paz, J. L., & Nieto, P. M. (2014) · European Journal of Organic Chemistry · doi:10.1002/ejoc.201402222 · Cited by 35 · see citing works
Blood-Stage Malaria Parasite Antigens: Structure, Function, and Vaccine Potential
Salinas, N. D., Tang, W. K., & Tolia, N. H. (2019) · Journal of Molecular Biology · doi:10.1016/j.jmb.2019.05.018 · Cited by 34 · see citing works
Faller, C. E., & Guvench, O. (2015) · The Journal of Physical Chemistry B · doi:10.1021/jp511431q · Cited by 34 · see citing works
Dechavanne, S., Srivastava, A., Gangnard, S., Nunes-Silva, S., Dechavanne, C., Fiévet, N., Deloron, P., Chêne, A., & Gamain, B. (2015) · Infection and Immunity · doi:10.1128/iai.03116-14 · Cited by 31 · see citing works
Avril, M., Cartwright, M. M., Hathaway, M. J., & Smith, J. D. (2011) · Malaria Journal · doi:10.1186/1475-2875-10-36 · Cited by 30 · see citing works
Tomlinson, A., Semblat, J., Gamain, B., & Chêne, A. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2020.624126 · Cited by 29 · see citing works
Juillerat, A., Igonet, S., Vigan-Womas, I., Guillotte-Blisnick, M., Gangnard, S., Faure, G., Baron, B., Raynal, B. D. E., Mercereau‐Puijalon, O., & Bentley, G. A. (2010) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2009.12.008 · Cited by 28 · see citing works
Achur, R. N., Kakizaki, I., Goel, S., Kojima, K., Madhunapantula, S. V., Goyal, A., Ohta, M., Kumar, S., Takagaki, K., & Gowda, D. C. (2008) · Biochemistry · doi:10.1021/bi801643m · Cited by 28 · see citing works
Self-citationCan any lessons be learned from the ambiguous glycan binding of PfEMP1 domains?
Dahlbäck, M., Nielsen, M. A., & Salanti, A. (2010) · Trends in Parasitology · doi:10.1016/j.pt.2010.02.002 · Cited by 28 · see citing works
Molecular Principles of Intrauterine Growth Restriction in Plasmodium Falciparum Infection
Seitz, J., Prieto, D. M. M., Fávaro, R. R., Schneider, H., & Markert, U. R. (2019) · Frontiers in Endocrinology · doi:10.3389/fendo.2019.00098 · Cited by 29 · see citing works
Renn, J. P., Doritchamou, J. Y. A., Tentokam, B. C. N., Morrison, R. D., Cowles, M. V., Burkhardt, M., Ma, R., Mahamar, A., Attaher, O., Diarra, B. S., et al. (2021) · Communications Biology · doi:10.1038/s42003-021-02787-7 · Cited by 24 · see citing works
Singh, K., Gitti, R. K., Diouf, A., Zhou, H., Gowda, D. C., Miura, K., Ostazeski, S. A., Fairhurst, R. M., Garboczi, D. N., & Long, C. A. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.118612 · Cited by 25 · see citing works
Self-citationGangnard, S., Lewit‐Bentley, A., Dechavanne, S., Srivastava, A., Amirat, F., Bentley, G. A., & Gamain, B. (2015) · Scientific Reports · doi:10.1038/srep14868 · Cited by 25 · see citing works
Combating malaria with nanotechnology-based targeted and combinatorial drug delivery strategies
Thakkar, M., & Brijesh, S. (2016) · Drug Delivery and Translational Research · doi:10.1007/s13346-016-0290-2 · Cited by 25 · see citing works
Pre-clinical and clinical development of the first placental malaria vaccine
Pehrson, C., Salanti, A., Theander, T. G., & Nielsen, M. A. (2017) · Expert Review of Vaccines · doi:10.1080/14760584.2017.1322512 · Cited by 25 · see citing works
Catabolism of chondroitin sulfate
Yamada, S. (2015) · Cellular & Molecular Biology Letters · doi:10.1515/cmble-2015-0011 · Cited by 23 · see citing works
Antigen Reversal Identifies Targets of Opsonizing IgGs against Pregnancy-Associated Malaria
Lambert, L. H., Bullock, J. L., Cook, S. T., Miura, K., Garboczi, D. N., Diakité, M., Fairhurst, R. M., Singh, K., & Long, C. A. (2014) · Infection and Immunity · doi:10.1128/iai.02097-14 · Cited by 23 · see citing works
Self-citationCarbohydrate binding molecules in malaria pathology
Brown, A., & Higgins, M. K. (2010) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2010.06.008 · Cited by 23 · see citing works
Developing vaccines to prevent malaria in pregnant women
Ndam, N. T., & Deloron, P. (2015) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2015.1049595 · Cited by 22 · see citing works
Apicomplexan parasite adhesins: novel strategies for targeting host cell carbohydrates
Boulanger, M. J., Tonkin, M. L., & Crawford, J. (2010) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2010.08.003 · Cited by 21 · see citing works
Obiakor, H., Avril, M., MacDonald, N. J., Srinivasan, P., Reiter, K., Anderson, C., Holmes, K. L., Fried, M., Duffy, P. E., Smith, J. D., et al. (2013) · Clinical and Vaccine Immunology · doi:10.1128/cvi.00638-12 · Cited by 22 · see citing works
Self-citationOptimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris
Avril, M., Hathaway, M. J., Cartwright, M. M., Gose, S. O., Narum, D. L., & Smith, J. D. (2009) · Malaria Journal · doi:10.1186/1475-2875-8-143 · Cited by 21 · see citing works
Gullingsrud, J., Saveria, T., Amos, E., Duffy, P. E., & Oleinikov, A. V. (2013) · PLoS ONE · doi:10.1371/journal.pone.0061323 · Cited by 21 · see citing works
Plasmodium falciparum has evolved multiple mechanisms to hijack human immunoglobulin M
Ji, C., Shen, H., Su, C., Li, Y., Chen, S., Sharp, T. H., & Xiao, J. (2023) · Nature Communications · doi:10.1038/s41467-023-38320-z · Cited by 19 · see citing works
Kane, E. G., & Taylor-Robinson, A. W. (2011) · Malaria Research and Treatment · doi:10.4061/2011/764845 · Cited by 19 · see citing works
Samantray, S., Olubiyi, O. O., & Strodel, B. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222111529 · Cited by 19 · see citing works
Siddiqui, A. A., Xainli, J., Schloegel, J., Carias, L. L., Ntumngia, F. B., Shoham, M., Casey, J. L., Foley, M., Adams, J. H., & King, C. L. (2012) · Infection and Immunity · doi:10.1128/iai.00206-12 · Cited by 17 · see citing works
RetractedFunctionally Relevant Proteins in Plasmodium Falciparum Host Cell Invasion
Patarroyo, M. E., Alba, M. P., Rojas-Luna, R., Bermúdez, A., & Aza-Conde, J. (2017) · Immunotherapy · doi:10.2217/imt-2016-0091 · Cited by 17 · see citing works
Wang, W., Wang, Z., Yang, X., Gao, Y., Zhang, X., Wang, W., Zhao, L., zhang, J., Jing, Q., Xu, Q., et al. (2021) · Cell Discovery · doi:10.1038/s41421-021-00324-8 · Cited by 17 · see citing works
de Brevern, A. G., Autin, L., Colin, Y., Bertrand, O., & Etchebest, C. (2009) · Infectious Disorders - Drug Targets · doi:10.2174/1871526510909030289 · Cited by 17 · see citing works
Gill, J., Chitnis, C. E., & Sharma, A. K. (2009) · Malaria Journal · doi:10.1186/1475-2875-8-67 · Cited by 16 · see citing works
Bewley, M. C., Gautam, L., Jagadeeshaprasad, M. G., Gowda, D. C., & Flanagan, J. M. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra120.014676 · Cited by 15 · see citing works
Self-citationVAR2CSA Signatures of High Plasmodium falciparum Parasitemia in the Placenta
Rovira-Vallbona, E., Monteiro, I. F., Bardají, A., Serra‐Casas, E., Neafsey, D. E., Quelhas, D., Valim, C., Alonso, P. L., Dobaño, C., Ordï, J., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069753 · Cited by 14 · see citing works
Multiple interests in structural models of DARC transmembrane protein
Smolarek, D., Bertrand, O., Czerwiński, M., Colin, Y., Etchebest, C., & de Brevern, A. G. (2010) · Transfusion Clinique et Biologique · doi:10.1016/j.tracli.2010.05.003 · Cited by 14 · see citing works
Bora, H., Garg, S., Sen, P., Kumar, D., Kaur, P., Khan, R. H., & Sharma, Y. D. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016294 · Cited by 14 · see citing works
Victor, M. E., Bengtsson, A., Andersen, G., Bengtsson, D. C., Lusingu, J., Vestergaard, L. S., Arnot, D. E., Theander, T. G., Joergensen, L., & Jensen, A. T. (2010) · Malaria Journal · doi:10.1186/1475-2875-9-325 · Cited by 14 · see citing works
Structural insights into chondroitin sulfate binding in pregnancy-associated malaria
Khunrae, P., & Higgins, M. K. (2010) · Biochemical Society Transactions · doi:10.1042/bst0381337 · Cited by 14 · see citing works
Atomic fidelity of subunit-based chemically-synthesized antimalarial vaccine components
Patarroyo, M. E., Cifuentes, G., Martínez, N. L., & Patarroyo, M. A. (2009) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2009.10.006 · Cited by 14 · see citing works
Talundzic, E., Shah, S., Fawole, O., Owino, S. O., Moore, J. M., & Peterson, D. S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031565 · Cited by 13 · see citing works
Oleinikov, A. V. (2022) · Biochemistry (Moscow) · doi:10.1134/s0006297922140152 · Cited by 12 · see citing works
Gangnard, S., Ndam, N. T., Gnidehou, S., Quiviger, M., Juillerat, A., Faure, G., Baron, B., Viwami, F., Deloron, P., & Bentley, G. A. (2010) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2010.05.014 · Cited by 12 · see citing works
How does Plasmodium falciparum stick to CSA? Let's see in the crystal
Bentley, G. A., & Gamain, B. (2008) · Nature Structural & Molecular Biology · doi:10.1038/nsmb0908-895 · Cited by 9 · see citing works
Using the PfEMP1 Head Structure Binding Motif to Deal a Blow at Severe Malaria
Patarroyo, M. E., Alba, M. P., Curtidor, H., Vanegas, M., Almonacid, H., & Patarroyo, M. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088420 · Cited by 8 · see citing works
How specific is Plasmodium falciparum adherence to chondroitin 4-sulfate?
Goel, S., & Gowda, D. C. (2011) · Trends in Parasitology · doi:10.1016/j.pt.2011.03.005 · Cited by 8 · see citing works
Self-citationIyamu, U., Vinals, D. F., Tornyigah, B., Arango, E., Bhat, R., Adra, T. R., Grewal, S., Martin, K., Maestre, A., Overduin, M., et al. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2023.1202276 · Cited by 7 · see citing works
Jagadeeshaprasad, M. G., Gautam, L., Bewley, M. C., Goel, S., Akhouri, R. R., & Gowda, D. C. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.11.258 · Cited by 7 · see citing works
Self-citationAntigenic Variation, Adherence, and Virulence in Malaria
Smith, J. D., & Deitsch, K. (2012) · Wiley eBooks · doi:10.1002/9781118308165.ch18 · Cited by 6 · see citing works
Padilla, A., Dovell, S., Chesnokov, O., Hoggard, M., Oleinikov, A. V., & Marí, F. (2020) · Journal of Proteomics · doi:10.1016/j.jprot.2020.104083 · Cited by 6 · see citing works
Guillotte-Blisnick, M., Juillerat, A., Igonet, S., Hessel, A., Pêtres, S., Crublet, E., Scanf, C. L., Lewit‐Bentley, A., Bentley, G. A., Vigan-Womas, I., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0134292 · Cited by 5 · see citing works
Spadeto, J. P. M., Cormanich, R. A., França, T. C. C., LaPlante, S. R., & Gonçalves, A. D. S. (2021) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2021.1911859 · Cited by 3 · see citing works
Plasmodium falciparum has evolved multiple mechanisms to hijack human immunoglobulin MPreprint
Ji, C., Shen, H., Su, C., Li, Y., Chen, S., Sharp, T. H., & Xiao, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.08.03.502706 · Cited by 3 · see citing works
Roessner, R. A., Michelarakis, N., Gräter, F., & Aponte-Santamaria, C. A. (2023) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1011726 · Cited by 2 · see citing works
Chêne, A. (2009) · Figshare · Cited by 2 · see citing works
SURFACE ANTIGENS AND VIRULENCE IN PLASMODIUM FALCIPARUM MALARIA
Normark, J. (2008) · Figshare · Cited by 1 · see citing works
Nilsson, S. (2011) · Figshare · Cited by 1 · see citing works
Blomqvist, K. (2011) · Figshare
Pathology and Pathogenesis of Malaria
Amaratunga, C., Lopera-Mesa, T. M., Tse, J. G., Mita-Mendoza, N. K., & Fairhurst, R. M. (2014) · ASM Press eBooks · doi:10.1128/9781555816872.ch29
Ibitokou, A. A. A. S. (2013) · theses.fr (ABES) · doi:10.70675/b940f61dzee5dz46e1za646z2b6d29f41b9d
NMR Studies of Protein–Glycosaminoglycan Interactions
Wang, X. (2017) · Royal Society of Chemistry eBooks · doi:10.1039/9781782623946-00250
Smolarek, D., Bertrand, O., Czerwiński, M., Colin, Y., Etchebest, C., & de Brevern, A. G. (2010)
Molecular Dynamics Simulation and Analysis of some Ligands on Var2csA Target
Okeke, I. (2020) · Novel Approaches in Drug Designing & Development · doi:10.19080/napdd.2020.05.555666
Rovira-Vallbona, E. (2011) · Dipòsit Digital de la Universitat de Barcelona (Universitat de Barcelona)
Molecular Docking and Analysis of some Ligands on Var2csA Target
Okeke, I. (2022) · Journal of Health Medicine and Clinical Studies · doi:10.58425/jhmcs.v1i1.84
- Authors
- Singh, K., Gittis, A. G., Nguyen, P., Gowda, D. C., Miller, L. H., & Garboczi, D. N.
- Affiliations
- National Institutes of Health (US)
- Pennsylvania State University (US)
- Corresponding author
- Singh, Kavita
- Garboczi, David N
- Venue
- Nature Structural & Molecular Biology
- Published
- 2008-08-24
- Volume
- 15
- Issue
- 9
- Pages
- 932-938
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- PMC
- PMC2658892
- OpenAlex
- W2052057644
- Cited by
- 99
- References
- 40
- Citations by year
- 2023: 3 · 2022: 4 · 2021: 9 · 2020: 3 · 2019: 3 · 2017: 4 · 2016: 2 · 2015: 9 · 2014: 6 · 2013: 7 · 2012: 10
- FWCI
- 12.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Energy (W - 31 - 109 - Eng - 38)
- National Institutes of Health (AI45086)
- Office of Science (W-31-109-ENG-38)
- National Institute of Allergy and Infectious Diseases (R21AI045086, AI45086, R01AI045086, Z01AI000881)
- Basic Energy Sciences (W-31-109-Eng38)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Health Sciences › Medicine › Hematology › Blood groups and transfusion
- Keywords
- VAR2CSA, chondroitin sulfate A, malaria in pregnancy, Plasmodium falciparum, Duffy binding-like domain, carbohydrate-protein interactions, binding sites, lysine residues, vaccine targets, placental malaria
- MeSH terms
- Animals, Antigens, Protozoan, Binding Sites, Chondroitin Sulfates, Erythrocytes, Female, Humans, Infant, Newborn, Models, Molecular, Placenta, Plasmodium falciparum, Pregnancy, Pregnancy Complications, Parasitic, Protozoan Proteins, Mutagenesis, Site-Directed, Malaria, Falciparum, Protein Structure, Tertiary, Crystallography, X-Ray, Macromolecular Substances, Static Electricity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review