The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Biological basis for restriction of microRNA targets to the 3′ untranslated region in mammalian mRNAs
Open accessAbstract
MicroRNAs (miRNAs) interact with target sites located in the 3′ untranslated regions (3′ UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to target mRNA. To establish the functional importance of target-site localization in the 3′ UTR, we modified the stop codon to extend the coding region of the transgene reporter through the miRNA target sequence. As a result, the miRNAs lost their ability to inhibit translation but retained their ability to function as small interfering RNAs in mammalian cells in culture and in vivo. The addition of rare but not optimal codons upstream of the extended opening reading frame (ORF) made the miRNA target site more accessible and restored miRNA-induced translational knockdown. Taken together, these results suggest that active translation impedes miRNA-programmed RISC association with target mRNAs and support a mechanistic explanation for the localization of most miRNA target sites in noncoding regions of mRNAs in mammals.
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Self-citationc-Myc-regulated microRNAs modulate E2F1 expression
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Predicting effective microRNA target sites in mammalian mRNAs
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A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language?
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Regulation of mRNA Translation and Stability by microRNAs
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Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIP
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Metadata
- Authors
- Gu, S., Jin, L., Zhang, F., Sarnow, P., & Kay, M. A.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2009-02
- Volume
- 16
- Issue
- 2
- Pages
- 144-150
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W1997379339
- Cited by
- 462
- References
- 49
- FWCI
- 11.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Gu, S., Jin, L., Zhang, F., Sarnow, P., & Kay, M. A. (2009). Biological basis for restriction of microRNA targets to the 3′ untranslated region in mammalian mRNAs. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1552
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