Diversifying microRNA sequence and function
Abstract
MicroRNAs (miRNAs) regulate the expression of most genes in animals, but we are only now beginning to understand how they are generated, assembled into functional complexes and destroyed. Various mechanisms have now been identified that regulate miRNA stability and that diversify miRNA sequences to create distinct isoforms. The production of different isoforms of individual miRNAs in specific cells and tissues may have broader implications for miRNA-mediated gene expression control. Rigorously testing the many discrepant models for how miRNAs function using quantitative biochemical measurements made in vivo and in vitro remains a major challenge for the future.
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PCR enrichment using a primer targeted to the miRNA causes final reads to lose the original miRNA sequence present in the miRNA:mRNA chimeric molecule, leading to loss of information about differential targeting of miRNA family members and isomiRs.
“Next, because of the use of a primer targeted to the miRNA, the final reads reflect amplified products and have lost the original miRNA sequence present in the miRNA:mRNA chimeric molecule, leading to loss of information about differential targeting of miRNA family members (which often only contain a single nucleotide difference), and isomiRs or other variations which have been described to play”
From Scalable and deep profiling of mRNA targets for individual microRNAs with chimeric eCLIP (2022) · cites this paper as Ameres and Zamore, 2013
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- Authors
- Ameres, S. L., & Zamore, P. D.
- Affiliations
- Institute of Molecular Biotechnology (AT)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Ameres, Stefan L.
- Zamore, Phillip D.
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2013-06-26
- Volume
- 14
- Issue
- 8
- Pages
- 475-488
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- OpenAlex
- W2078849828
- Cited by
- 1,175
- References
- 16
- Citations by year
- 2026: 14 · 2025: 36 · 2024: 46 · 2023: 46 · 2022: 71 · 2021: 77 · 2020: 97 · 2019: 104 · 2018: 122 · 2017: 156 · 2016: 127 · 2015: 155 · 2014: 109 · 2013: 14 · 2012: 1
- FWCI
- 11.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of General Medical Sciences (R01GM065236)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- microRNA, miRNA stability, miRNA isoforms, gene expression regulation, microRNA biogenesis
- MeSH terms
- Animals, Humans, Models, Biological, RNA Processing, Post-Transcriptional, Transcription, Genetic, Genetic Variation, Gene Expression, RNA Stability, MicroRNAs
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