The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
STIM1 gates the store-operated calcium channel ORAI1 in vitro
Open accessAbstract
Store-operated Ca2+ entry through the plasma membrane Ca2+ release–activated Ca2+ (CRAC) channel in mammalian T cells and mast cells depends on the sensor protein stromal interaction molecule 1 (STIM1) and the channel subunit ORAI1. To study STIM1-ORAI1 signaling in vitro, we have expressed human ORAI1 in a sec6-4 strain of the yeast Saccharomyces cerevisiae and isolated sealed membrane vesicles carrying ORAI1 from the Golgi compartment to the plasma membrane. We show by in vitro Ca2+ flux assays that bacterially expressed recombinant STIM1 opens wild-type ORAI1 channels but not channels assembled from the ORAI1 pore mutant E106Q or the ORAI1 severe combined immunodeficiency (SCID) mutant R91W. These experiments show that the STIM1-ORAI1 interaction is sufficient to gate recombinant human ORAI1 channels in the absence of other proteins of the human ORAI1 channel complex, and they set the stage for further biochemical and biophysical dissection of ORAI1 channel gating.
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Cites
Store-Operated Calcium Channels
Parekh, A. B., & Putney, J. W. (2005) · Physiological Reviews · doi:10.1152/physrev.00057.2003 · Cited by 2,089
Calcium signalling in lymphocyte activation and disease
Feske, S. (2007) · Nature Reviews Immunology · doi:10.1038/nri2152 · Cited by 1,043
A Homolog of Voltage-Gated Ca 2+ Channels Stimulated by Depletion of Secretory Ca 2+ in Yeast
Locke, E. G., Bonilla, M., Liang, L., Takita, Y., & Cunningham, K. W. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6686-6694.2000 · Cited by 212
Dissecting ICRAC, a store-operated calcium current
Hogan, P. G., & Rao, A. (2007) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.03.009 · Cited by 117
Self-citation
Cited by
Store-Operated Calcium Channels
Prakriya, M., & Lewis, R. S. (2015) · Physiological Reviews · doi:10.1152/physrev.00020.2014 · Cited by 1,239
Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI
Hogan, P. G., Lewis, R. S., & Rao, A. (2010) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132550 · Cited by 760
Self-citationOrganization and Function of Membrane Contact Sites
Helle, S. C. J., Kanfer, G., Kolar, K., Lang, A., Michel, A. H., & Kornmann, B. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2013.01.028 · Cited by 453
Diseases caused by mutations in ORAI1 and STIM1
Lacruz, R. S., & Feske, S. (2015) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.12938 · Cited by 443
SARAF Inactivates the Store Operated Calcium Entry Machinery to Prevent Excess Calcium Refilling
Palty, R., Raveh, A., Kaminsky, I., Meller, R., & Reuveny, E. (2012) · Cell · doi:10.1016/j.cell.2012.01.055 · Cited by 273
Metadata
- Authors
- Zhou, Y., Meraner, P., Kwon, H. T., Machnes, D., Oh-hora, M., Zimmer, J., Huang, Y., Stura, A., Rao, A., & Hogan, P. G.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2009-12-27
- Volume
- 17
- Issue
- 1
- Pages
- 112-116
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2093214713
- Cited by
- 239
- References
- 4
- FWCI
- 7.71
- Type
- journal article
- Language
- en
- Open access
- green
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