Perilipin Expression in Human Adipose Tissues: Effects of Severe Obesity, Gender, and Depot
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Objective: Perilipins are phosphoproteins that are localized to the surface of triacylglycerol droplets within adipocytes where they regulate the rate of lipolysis. We sought to determine the effects of severe obesity and depot [omental (Om) vs. subcutaneous (Sc)] on perilipin expression in the adipose tissue of individuals. Research Methods and Procedures: Samples of Om and Sc adipose tissues obtained at surgery from severely obese subjects and fat aspirations from nonobese subjects were analyzed for perilipin protein and mRNA levels by Northern and Western analysis. Results: Perilipin A (periA) was the major perilipin expressed in adipose tissues. periA mRNA relative abundance was significantly lower in Sc adipose tissue from severely obese compared to that from nonobese subjects. Western blotting of adipose tissue extracts showed that periA protein levels expressed relative to tissue protein or fat cell surface area were significantly lower (∼ −40%) in abdominal Sc adipose tissue from severely obese compared to that from nonobese subjects. However, the calculated mass of perilipin per fat cell did not differ between the two groups. Perilipin mRNA levels were higher in Sc compared to Om adipose tissue from obese individuals (p < 0.025; n = 26; 17 women, 9 men); however, periA protein levels did not differ. In addition, perilipin protein, but not mRNA, levels were higher in Sc adipose tissue from obese men than from women (p < 0.025). Discussion: Variations in perilipin expression may contribute to the higher basal lipolytic rates observed in obese compared to nonobese individuals and in obese women compared to obese men.
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Abdominal subcutaneous adipose perilipin protein relative to cell surface area is greater in obese men than in obese women.
“Abdominal subcutaneous adipose perilipin protein relative to cell surface area was found to be greater in obese men than in obese women (60).”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [60]
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- Authors
- Wang, Y., Sullivan, S., Trujillo, M., Lee, M., Schneider, S. H., Brolin, R. E., Kang, Y. H., Werber, Y., Greenberg, A. S., & Fried, S. K.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Tufts University (US)
- Corresponding author
- Fried, Susan K.
- Venue
- Obesity Research
- Publisher
- Wiley
- Published
- 2003-08
- Volume
- 11
- Issue
- 8
- Pages
- 930-936
- ISSN
- 1071-7323 (ISSN-L)
- 1550-8528
- OpenAlex
- W2028942296
- Cited by
- 128
- References
- 28
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 7 · 2018: 2 · 2017: 11 · 2016: 5 · 2015: 6 · 2014: 6 · 2013: 4 · 2012: 6
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Obesity Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Diabetes Association
- U.S. Department of Agriculture (58-1950-9-001)
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (DK52398, R01DK052398, DK50647, R01DK050647)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- perilipin, perilipin A, morbid obesity, adipose tissue, omental adipose tissue, subcutaneous adipose tissue, depots, lipolysis, adipocytes, gender differences, gene expression
- MeSH terms
- Perilipin-1, Adipose Tissue, Adult, Amino Acid Sequence, Carrier Proteins, Cell Count, Female, Humans, Male, Molecular Sequence Data, Molecular Weight, Obesity, Phosphoproteins, RNA, Messenger, Sex Factors, Blotting, Northern, Blotting, Western, Adipocytes
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