Fecal microbial transplantation and a high fiber diet attenuates emphysema development by suppressing inflammation and apoptosis
Open accessAbstract
Recent work has suggested a microbial dysbiosis association between the lung and gut in respiratory diseases. Here, we demonstrated that gut microbiome modulation attenuated emphysema development. To modulate the gut microbiome, fecal microbiota transplantation (FMT) and diet modification were adopted in mice exposed to smoking and poly I:C for the emphysema model. We analyzed the severity of emphysema by the mean linear intercept (MLI) and apoptosis by the fluorescent TUNEL assay. Microbiome analysis was also performed in feces and fecal extracellular vesicles (EVs). The MLI was significantly increased with smoking exposure. FMT or a high-fiber diet (HFD) attenuated the increase. Weight loss, combined with smoking exposure, was not noted in mice with FMT. HFD significantly decreased macrophages and lymphocytes in bronchoalveolar lavage fluid. Furthermore, IL-6 and IFN-γ were decreased in the bronchoalveolar lavage fluid and serum. The TUNEL score was significantly lower in mice with FMT or HFD, suggesting decreased cell apoptosis. In the microbiome analysis, Bacteroidaceae and Lachnospiraceae, which are alleged to metabolize fiber into short-chain fatty acids (SCFAs), increased at the family level with FMT and HFD. FMT and HFD attenuated emphysema development via local and systemic inhibition of inflammation and changes in gut microbiota composition, which could provide a new paradigm in COPD treatment.
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In a mouse model, a combination of FMT and a high-fiber diet attenuates the development of emphysema by downregulating inflammation and apoptosis.
“A combination of FMT and a high-fiber diet also attenuates the development of emphysema by downregulating inflammation and apoptosis in a mouse model. 114”
From Key determinants of success in fecal microbiota transplantation: From microbiome to clinic (2023) · cites this paper as [114]
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Leveraging diet to engineer the gut microbiome
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- Authors
- Jang, Y. O., Lee, S. H., Choi, J. J., Kim, D.-H., Choi, J.-M., Kang, M.-J., Oh, Y.-M., Park, Y.-J., Shin, Y., & Lee, S. W.
- Affiliations
- Asan Medical Center (KR)
- University of Ulsan (KR)
- Hanyang University (KR)
- Yale University (US)
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Corresponding author
- Shin, Yong
- Lee, Sei Won
- ORCID
- Venue
- Experimental & Molecular Medicine
- Published
- 2020-07
- Volume
- 52
- Issue
- 7
- Pages
- 1128-1139
- ISSN
- 1226-3613 (ISSN-L)
- 2092-6413
- PMC
- PMC8080776
- OpenAlex
- W3042783890
- Cited by
- 121
- References
- 63
- Citations by year
- 2026: 14 · 2025: 26 · 2024: 37 · 2023: 19 · 2022: 14 · 2021: 10 · 2020: 1
- FWCI
- 6.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Experimental & Molecular Medicine (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Foundation of Korea (2019R1F1A1057875, 2016R1A6A3A11932575)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Keywords
- fecal microbiota transplantation, high-fiber diet, emphysema, gut-lung axis, gut microbiota, smoking, poly(I:C), chronic obstructive pulmonary disease, inflammation, apoptosis, short-chain fatty acids, Bacteroidaceae, Lachnospiraceae, extracellular vesicles, interleukin-6, interferon-gamma, bronchoalveolar lavage
- MeSH terms
- Fecal Microbiota Transplantation, Administration, Oral, Animals, Emphysema, Fatty Acids, Feces, Female, Inflammation, Mice, Inbred C57BL, Pulmonary Alveoli, Weight Loss, Apoptosis, Diet, High-Fat
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