miRNA-3473b contributes to neuroinflammation following cerebral ischemia.
Open accessAbstract
MicroRNAs play an essential role in stroke pathology. Here, we investigated the role of a newly identified microRNA, miR-3473b, in stroke pathology. The expression of miR-3473b was upregulated in the cortex and striatum in mice following transient middle cerebral artery occlusion (MCAO). Intracerebroventricular injection of the miR-3473b antagomir prior to MCAO remarkably attenuated ischemia-induced expression of miR-3473b and pro-inflammatory factors in the ischemic brain and decreased infarct volumes in mice following MCAO. Using in vitro approaches, we showed that the miR-3473b antagomir reduced the mRNA and protein levels of pro-inflammatory factors (iNOS, COX-2, TNF-α, and IL-6) in BV2 microglial cells subjected to LPS stimulation. The miR-3473b antagomir also decreased the expression of pro-inflammatory factors in BV2 cells activated with conditioned medium collected from oxygen-glucose deprivation (OGD)-treated neurons. Suppressor of cytokine signaling 3 (SOCS3), a physiological regulator of innate and adaptive immunity, was predicted to be a potential target of miR-3473b. We verified that the miR-3473b mimic decreased SOCS3 expression in BV2 cells. Meanwhile, the miR-3473b antagomir significantly increased both SOCS3 mRNA and protein levels in the BV2 cells treated with LPS as well as in the ischemic brain. By using the dual luciferase assay, we further showed that the 3'-untranslational region of SOCS3 was directly targeted by miR-3473b. In conclusion, induction of miR-3473b, which is likely targeted to SOCS3, contributes to stroke pathogenesis by enhancing post-stroke neuroinflammation injury.
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Microglia shows excessive activation in pathological conditions such as chronic stress or infection, resulting in increased levels of inflammatory chemicals in the brain and neuronal damage and death.
“Microglia shows excessive activation in pathological condition such as chronic stress or infection, resulting increased not only in levels of inflammatory chemicals in the brain, but also neuronal damage and death [10].”
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Cites
The Science of Stroke: Mechanisms in Search of Treatments
Moskowitz, M. A., Lo, E. H., & Iadecola, C. (2010) · Neuron · doi:10.1016/j.neuron.2010.07.002 · Cited by 1,930 · see citing works
Reactive Oxygen Radicals in Signaling and Damage in the Ischemic Brain
Chan, P. H. (2001) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-200101000-00002 · Cited by 1,589 · see citing works
Hu, X.-M., Li, P.-Y., Guo, Y., Wang, H., Leak, R., Chen, S., Gao, Y.-Q., & Chen, J. (2012) · Stroke · doi:10.1161/strokeaha.112.659656 · Cited by 1,445 · see citing works
Self-citationThe biphasic function of microglia in ischemic stroke
Ma, Y., Wang, J., Wang, Y., & Yang, G. (2016) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2016.01.005 · Cited by 724 · see citing works
Inflammation in stroke and focal cerebral ischemia
Huang, J., Upadhyay, U. M., & Tamargo, R. J. (2006) · Surgical Neurology · doi:10.1016/j.surneu.2005.12.028 · Cited by 649 · see citing works
Jin, Q., Cheng, J. G., Liu, Y., Wu, J. H., Wang, X., Wei, S., Zhou, X., Qin, Z., Jia, J., & Zhen, X. (2014) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2014.03.003 · Cited by 294 · see citing works
Self-citationSOCS1 and SOCS3 in the control of CNS immunity
Baker, B. J., Akhtar, L. N., & Benveniste, E. N. (2009) · Trends in Immunology · doi:10.1016/j.it.2009.07.001 · Cited by 276 · see citing works
MicroRNA in ischemic stroke etiology and pathology
Rink, C. L., & Khanna, S. (2010) · Physiological Genomics · doi:10.1152/physiolgenomics.00158.2010 · Cited by 255 · see citing works
Saugstad, J. A. (2010) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2010.101 · Cited by 223 · see citing works
MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia
Taj, S. H., Kho, W., Riou, A., Wiedermann, D., & Hoehn, M. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.03.025 · Cited by 191 · see citing works
In Vivo Inhibition of miR-155 Promotes Recovery after Experimental Mouse Stroke
Caballero‐Garrido, E., Peña-Philippides, J. C., Lordkipanidze, T. G., Bragin, D. E., Yang, Y., Erhardt, E. B., & Roitbak, T. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.1641-15.2015 · Cited by 181 · see citing works
Liu, P., Zhao, H., Wang, R., et al. (2014) · Stroke · doi:10.1161/strokeaha.114.007482 · Cited by 181 · see citing works
Zhao, H., Wang, J., Gao, L., Wang, R., Liu, X., Gao, Z., Tao, Z., Xu, C., Song, J., Ji, X., et al. (2013) · Stroke · doi:10.1161/strokeaha.111.000504 · Cited by 173 · see citing works
Ni, J., Wang, X., Chen, S., Liu, H., Wang, Y., Xu, X., Cheng, J. G., Jia, J., & Zhen, X. (2015) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2015.04.014 · Cited by 169 · see citing works
Self-citationQin, H., Roberts, K. L., Niyongere, S., Cong, Y., Elson, C. O., & Benveniste, E. N. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.9.5966 · Cited by 168 · see citing works
Wu, C., Xue, Y., Wang, P., Lin, L., Liu, Q., Li, N., Xu, J., & Cao, X. (2014) · The Journal of Immunology · doi:10.4049/jimmunol.1302379 · Cited by 147 · see citing works
microRNAs: Innovative Targets for Cerebral Ischemia and Stroke
Ouyang, Y., Stary, C. M., Yang, G., & Giffard, R. G. (2012) · Current Drug Targets · doi:10.2174/138945013804806424 · Cited by 139 · see citing works
Mechanisms and Potential Therapeutic Applications of Microglial Activation after Brain Injury
Kim, J. Y., Kim, N., & Yenari, M. A. (2014) · CNS Neuroscience & Therapeutics · doi:10.1111/cns.12360 · Cited by 119 · see citing works
SOCS3 and its role in associated diseases
Yin, Y., Liu, W., & Dai, Y. (2015) · Human Immunology · doi:10.1016/j.humimm.2015.09.037 · Cited by 113 · see citing works
MicroRNAs: Key Players in Microglia and Astrocyte Mediated Inflammation in CNS Pathologies
Karthikeyan, A., Patnala, R., Jadhav, S. P., Ling, E., & Dheen, S. T. (2016) · Current Medicinal Chemistry · doi:10.2174/0929867323666160814001040 · Cited by 100 · see citing works
Doeppner, T. R., Kaltwasser, B., Bähr, M., & Hermann, D. M. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00338 · Cited by 89 · see citing works
miR-19a: An Effective Regulator of SOCS3 and Enhancer of JAK-STAT Signalling
Collins, A. S., McCoy, C. E., Lloyd, A. T., O’Farrelly, C., & Stevenson, N. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069090 · Cited by 89 · see citing works
Neuroprotective effect of microRNA-99a against focal cerebral ischemia–reperfusion injury in mice
Tao, Z., Zhao, H., Wang, R., et al. (2015) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2015.05.036 · Cited by 86 · see citing works
MicroRNA-200c Contributes to Injury From Transient Focal Cerebral Ischemia by Targeting Reelin
Stary, C. M., Xu, L., Sun, X., Ouyang, Y., White, R., Leong, J., Li, J., Xiong, X., & Giffard, R. G. (2015) · Stroke · doi:10.1161/strokeaha.114.007041 · Cited by 81 · see citing works
Tan, M., Yu, J., Jiang, T., Zhu, X., Guan, H., & Tan, L. (2013) · Journal of Alzheimer s Disease · doi:10.3233/jad-131148 · Cited by 80 · see citing works
Zhao, Y., Yu, J., Li, Q., Ma, C., Lü, C., & Xiao, B. (2011) · Neuron Glia Biology · doi:10.1017/s1740925x12000129 · Cited by 65 · see citing works
Anti-inflammatory effects of glaucocalyxin B in microglia cells
Gan, P., Li, Z., Chen, Y., Zhang, Y., Zhang, F., Zhou, X., Zhang, X., Gao, B., Zhen, X., Zhang, J., et al. (2015) · Journal of Pharmacological Sciences · doi:10.1016/j.jphs.2015.04.005 · Cited by 56 · see citing works
Self-citationSphingosine kinase 1 mediates neuroinflammation following cerebral ischemia
Zheng, S., Wei, S., Wang, X., et al. (2015) · Experimental Neurology · doi:10.1016/j.expneurol.2015.03.012 · Cited by 52 · see citing works
Self-citationZhang, B., Fang, L., Wu, H., Ding, P., Xu, K., & Liu, R. (2016) · Molecular Immunology · doi:10.1016/j.molimm.2016.06.016 · Cited by 40 · see citing works
Qin, X., Sun, Z.-Q., Dai, X., Mao, S., Zhang, J., Jia, M., & Zhang, Y.-M. (2012) · Neurological Research · doi:10.1179/1743132812y.0000000028 · Cited by 29 · see citing works
Xu, Z., Wu, J., Zheng, J., Ma, H., Zhang, H., Zhen, X., Zheng, L. T., & Zhang, X. (2015) · International Immunopharmacology · doi:10.1016/j.intimp.2015.02.033 · Cited by 28 · see citing works
Self-citationCalcium/calmodulin‐dependent protein kinase kinase β is neuroprotective in stroke in aged mice
Liu, L., Yuan, H., Denton, K. R., et al. (2016) · European Journal of Neuroscience · doi:10.1111/ejn.13299 · Cited by 19 · see citing works
Correction: SOCS3 Deficiency Promotes M1 Macrophage Polarization and Inflammation
Qin, H., Holdbrooks, A. T., Liu, Y., et al. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1600710 · Cited by 19 · see citing works
Choi, J., Shin, Y., Cha, J., Kim, H.-Y., Choi, J.-Y., Chun, M., & Lee, M. (2008) · Neuroscience Letters · doi:10.1016/j.neulet.2008.06.051 · Cited by 12 · see citing works
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Yang, R., Yang, B., Liu, W., Tan, C., Chen, H., & Wang, X. (2023) · Journal of Neuroinflammation · doi:10.1186/s12974-023-02856-0 · Cited by 91 · see citing works
Self-citationInhibition of activated astrocyte ameliorates lipopolysaccharide- induced depressive-like behaviors
Wang, Y., Ni, J., Zhai, L., Gao, C., Xie, L., Zhao, L., & Yin, X. (2019) · Journal of Affective Disorders · doi:10.1016/j.jad.2018.08.015 · Cited by 75 · see citing works
Self-citationWu, Z., Cai, Z., Shi, H., Huang, X., Cai, M., Yuan, K., Huang, P., Shi, G., Yan, T., & Li, Z. (2022) · Aging · doi:10.18632/aging.204030 · Cited by 37 · see citing works
- Authors
- Wang, X., Chen, S., Ni, J., Cheng, J., Jia, J., & Zhen, X.
- Affiliations
- Soochow University (CN)
- Corresponding author
- Jia, Jia
- Zhen, Xuechu
- Venue
- Cell Death & Disease
- Published
- 2018-01-09
- Volume
- 9
- Issue
- 1
- Pages
- 11-11
- ISSN
- 2041-4889 (ISSN-L)
- PMC
- PMC5849032
- OpenAlex
- W2783391945
- Cited by
- 110
- References
- 34
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 9 · 2023: 14 · 2022: 18 · 2021: 16 · 2020: 20 · 2019: 15 · 2018: 7
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Cell Death and Disease (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Natural Science Foundation of China (81671310, 81571124, BM2013003, 81373382, 81471336, 81371278, 81773702)
- Priority Academic Program Development of Jiangsu Higher Education Institutions (BM2013003, 81571124, 81373382, 81371278)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- microRNA, neuroinflammation, cerebral ischemia, stroke, SOCS3, microglia, BV-2 cells, middle cerebral artery occlusion, oxygen-glucose deprivation, inflammatory cytokines, inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, interleukin-6, antagomir, 3' untranslated region
- MeSH terms
- Antagomirs, Suppressor of Cytokine Signaling 3 Protein, Animals, Cells, Cultured, Brain Ischemia, Disease Models, Animal, Glucose, Male, Neurons, Tumor Necrosis Factor-alpha, Cell Hypoxia, Interleukin-6, Microglia, Infarction, Middle Cerebral Artery, 3' Untranslated Regions, MicroRNAs, Mice, Nitric Oxide Synthase Type II
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