Initiation of hepatic stellate cell activation extends into chronic liver disease
Open accessAbstract
Activated hepatic stellate cells (aHSC) are the main source of extra cellular matrix in liver fibrosis. Activation is classically divided in two phases: initiation and perpetuation. Currently, HSC-based therapeutic candidates largely focus on targeting the aHSCs in the perpetuation phase. However, the importance of HSC initiation during chronic liver disease (CLD) remains unclear. Here, we identified transcriptional programs of initiating and activated HSCs by RNA sequencing, using in vitro and in vivo mouse models of fibrosis. Importantly, we show that both programs are active in HSCs during murine and human CLD. In human cirrhotic livers, scar associated mesenchymal cells employ both transcriptional programs at the single cell level. Our results indicate that the transcriptional programs that drive the initiation of HSCs are still active in humans suffering from CLD. We conclude that molecules involved in the initiation of HSC activation, or in the maintenance of aHSCs can be considered equally important in the search for druggable targets of chronic liver disease.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Activated hepatic stellate cells are the primary source of extracellular matrix and a central factor in the development of liver fibrosis.
“The activated HSCs have long been recognized as the primary source of ECM and a central factor in the development of liver fibrosis (35).”
From Matrix mechanical remodeled carrier-free nanosystem for programmable closed-loop reversal of liver fibrosis via STING alkylation (2025) · cites this paper as [35]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
Love, M. I., Huber, W., & Anders, S. (2014) · Genome Biology · doi:10.1186/s13059-014-0550-8 · Cited by 95,106 · see citing works
Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625 · see citing works
STAR: ultrafast universal RNA-seq aligner
Dobin, A., Davis, C. A., Schlesinger, F., Drenkow, J., Zaleski, C., Jha, S., Batut, P., Chaisson, M., & Gingeras, T. R. (2013) · Bioinformatics · doi:10.1093/bioinformatics/bts635 · Cited by 51,709 · see citing works
limma powers differential expression analyses for RNA-sequencing and microarray studies
Ritchie, M. E., Phipson, B., Wu, D., Hu, Y., Law, C. W., Shi, W., & Smyth, G. K. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv007 · Cited by 40,458 · see citing works
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
Huang, D. W., Sherman, B. T., & Lempicki, R. A. (2009) · Nature Protocols · doi:10.1038/nprot.2008.211 · Cited by 35,890 · see citing works
Comprehensive Integration of Single-Cell Data
Stuart, T., Butler, A., Hoffman, P., Hafemeister, C., Papalexi, E., Mauck, W. M., Hao, Y., Stoeckius, M., Smibert, P., & Satija, R. (2019) · Cell · doi:10.1016/j.cell.2019.05.031 · Cited by 15,453 · see citing works
GSVA: gene set variation analysis for microarray and RNA-Seq data
Hänzelmann, S., Castelo, R., & Guinney, J. (2013) · BMC Bioinformatics · doi:10.1186/1471-2105-14-7 · Cited by 15,440 · see citing works
StringTie enables improved reconstruction of a transcriptome from RNA-seq reads
Pertea, M., Pertea, G. M., Antonescu, C. M., Chang, T.-C., Mendell, J. T., & Salzberg, S. L. (2015) · Nature Biotechnology · doi:10.1038/nbt.3122 · Cited by 14,118 · see citing works
affy—analysis of Affymetrix GeneChip data at the probe level
Gautier, L., Cope, L., Bolstad, B. M., & Irizarry, R. A. (2004) · Bioinformatics · doi:10.1093/bioinformatics/btg405 · Cited by 5,221 · see citing works
Villanueva, A. (2019) · New England Journal of Medicine · doi:10.1056/nejmra1713263 · Cited by 4,508 · see citing works
Mechanisms of hepatic stellate cell activation
Tsuchida, T., & Friedman, S. L. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.38 · Cited by 2,909 · see citing works
Molecular Regulation of Hepatic Fibrosis, an Integrated Cellular Response to Tissue Injury
Friedman, S. L. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.4.2247 · Cited by 2,090 · see citing works
(1977) · Annals of Internal Medicine · doi:10.7326/0003-4819-87-5-642_1 · Cited by 2,087 · see citing works
Molecular and cellular mechanisms of liver fibrosis and its regression
Kisseleva, T., & Brenner, D. (2021) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-020-00372-7 · Cited by 2,024 · cited 3× · see citing works
“LOXL2 or TIMP1 antagonists) phenotype of HSCs [7, 8].”
BackgroundResolving the fibrotic niche of human liver cirrhosis at single-cell level
Ramachandran, P., Dobie, R., Wilson-Kanamori, J. R., Dora, E. F., Henderson, B. E. P., Luu, N. T., Portman, J. R., Matchett, K. P., Brice, M., Marwick, J. A., et al. (2019) · Nature · doi:10.1038/s41586-019-1631-3 · Cited by 1,784 · see citing works
A framework for oligonucleotide microarray preprocessing
Carvalho, B. S., & Irizarry, R. A. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq431 · Cited by 1,554 · see citing works
Mederacke, I., Hsu, C. C., Troeger, J. S., Huebener, P., Mu, X., Dapito, D. H., Pradere, J.-P., & Schwabe, R. F. (2013) · Nature Communications · doi:10.1038/ncomms3823 · Cited by 1,443 · see citing works
Genome-wide association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growth
Zanconato, F., Forcato, M., Battilana, G., Azzolin, L., Quaranta, E., Bodega, B., Rosato, A., Bicciato, S., Cordenonsi, M., & Piccolo, S. (2015) · Nature Cell Biology · doi:10.1038/ncb3216 · Cited by 1,219 · see citing works
Pimpin, L., Cortez‐Pinto, H., Negro, F., Corbould, E., Lazarus, J. V., Webber, L., & Sheron, N. (2018) · Journal of Hepatology · doi:10.1016/j.jhep.2018.05.011 · Cited by 713 · see citing works
Pradere, J.-P., Kluwe, J., De Minicis, S., Jiao, J.-J., Gwak, G.-Y., Dapito, D. H., Jang, M.-K., Guenther, N. D., Mederacke, I., Friedman, R., et al. (2013) · Hepatology · doi:10.1002/hep.26429 · Cited by 613 · see citing works
Deactivation of Hepatic Stellate Cells During Liver Fibrosis Resolution in Mice
Troeger, J. S., Mederacke, I., Gwak, G., Dapito, D. H., Mu, X., Hsu, C. C., Pradere, J., Friedman, R. A., & Schwabe, R. F. (2012) · Gastroenterology · doi:10.1053/j.gastro.2012.06.036 · Cited by 515 · see citing works
Use of Liver Imaging and Biopsy in Clinical Practice
Tapper, E. B., & Lok, A. S.-F. (2017) · New England Journal of Medicine · doi:10.1056/nejmra1610570 · Cited by 452 · see citing works
The Power of Plasticity—Metabolic Regulation of Hepatic Stellate Cells
Trivedi, P., Wang, S., & Friedman, S. L. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2020.10.026 · Cited by 427 · see citing works
The Hippo pathway effector YAP controls mouse hepatic stellate cell activation
Mannaerts, I., Leite, S. B., Verhulst, S., Claerhout, S., Eysackers, N., Thoen, L. F. R., Hoorens, A., Reynaert, H., Halder, G., & van Grunsven, L. A. (2015) · Journal of Hepatology · doi:10.1016/j.jhep.2015.04.011 · Cited by 410 · see citing works
Self-citationRAP2 mediates mechanoresponses of the Hippo pathway
Meng, Z., Qiu, Y., Lin, K. C., Kumar, A., Placone, J. K., Fang, C., Wang, K.-C., Lu, S., Pan, M., Hong, A. W., et al. (2018) · Nature · doi:10.1038/s41586-018-0444-0 · Cited by 377 · see citing works
Krenkel, O., Hundertmark, J., Ritz, T., Weiskirchen, R., & Tacke, F. (2019) · Cells · doi:10.3390/cells8050503 · Cited by 241 · see citing works
Guimarães, E. L. M., Empsen, C., Geerts, A. E., & van Grunsven, L. A. (2009) · Journal of Hepatology · doi:10.1016/j.jhep.2009.12.007 · Cited by 226 · see citing works
Acute liver failure is associated with elevated liver stiffness and hepatic stellate cell activation
Dechêne, A., Sowa, J., Gieseler, R., Jochum, C., Bechmann, L., Fouly, A. E. E., Schlattjan, M., Saner, F., Baba, H., Paul, A., et al. (2010) · Hepatology · doi:10.1002/hep.23754 · Cited by 158 · see citing works
Orbit Image Analysis: An open-source whole slide image analysis tool
Stritt, M., Stalder, A. K., & Vezzali, E. (2020) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1007313 · Cited by 152 · see citing works
Heterogeneity of HSCs in a Mouse Model of NASH
Rosenthal, S. B., Liu, X., Ganguly, S., Dhar, D., Pasillas, M. P., Ricciardelli, E., Li, R. Z., Troutman, T. D., Kisseleva, T., Glass, C. K., et al. (2021) · Hepatology · doi:10.1002/hep.31743 · Cited by 145 · see citing works
Pont, F., Tosolini, M., & Fournié, J. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz601 · Cited by 131 · see citing works
Zhang, D. Y., Goossens, N., Guo, J., Tsai, M., Chou, H.-I., Altunkaynak, C., Sangiovanni, A., Iavarone, M. A., Colombo, M., Kobayashi, M., et al. (2015) · Gut · doi:10.1136/gutjnl-2015-309655 · Cited by 129 · see citing works
Unfolded protein response is an early, non-critical event during hepatic stellate cell activation
Mannaerts, I., Thoen, L. F. R., Eysackers, N., Cubero, F. J., Leite, S. B., Coldham, I., Colle, I. O., Trautwein, C., & van Grunsven, L. A. (2019) · Cell Death and Disease · doi:10.1038/s41419-019-1327-5 · Cited by 35 · see citing works
Self-citationAsakawa, M., Itoh, M., Suganami, T., Sakai, T., Kanai, S., Shirakawa, I., Yuan, X., Hatayama, T., Shimada, S., Akiyama, Y., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-56039-0 · Cited by 23 · see citing works
Huisman, T. M., Dieterich, D. T., & Friedman, S. L. (2021) · Journal of Experimental Pharmacology · doi:10.2147/jep.s265286 · Cited by 23 · see citing works
Flagged first, then by citations
1 of the 36 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Treatment of liver fibrosis: Past, current, and future
Zhang, C., Liu, S., & Yang, M. J. (2023) · World Journal of Hepatology · doi:10.4254/wjh.v15.i6.755 · Cited by 97 · see citing works
Current and emerging strategies for the prevention of hepatocellular carcinoma
Yeo, Y. H., Abdelmalek, M. F., Khan, S. A., Moylan, C. A., Rodriquez, L. M., Villanueva, A., & Yang, J. D. (2024) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-024-01021-z · Cited by 99 · see citing works
Yan, M., Cui, Y., & Xiang, Q. (2025) · Theranostics · doi:10.7150/thno.106597 · Cited by 76 · see citing works
Wang, Z., Li, Y., Wang, X., Zhang, W., Chen, Y., Lu, X., Jin, C., Tu, L., Jiang, T., Yang, Y., et al. (2025) · Phytomedicine · doi:10.1016/j.phymed.2025.156796 · Cited by 50 · see citing works
Li, X., Xie, E., Sun, S., Shen, J., Ding, Y., Wang, J., Peng, X., Zheng, R., Farag, M. A. F., & Xiao, J. (2025) · Journal of Advanced Research · doi:10.1016/j.jare.2025.01.014 · Cited by 46 · see citing works
TM7SF3 controls TEAD1 splicing to prevent MASH-induced liver fibrosis
Isaac, R., Bandyopadhyay, G. K., Rohm, T. V., Kang, S., Wang, J., Pokhrel, N. P., Sakane, S., Zapata, R. C., Libster, A. M., Vinik, Y., et al. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.04.003 · Cited by 34 · see citing works
Merens, V., Knetemann, E., Gürbüz, E., Smet, V. D., Messaoudi, N., Reynaert, H., Verhulst, S., & van Grunsven, L. A. (2024) · JHEP Reports · doi:10.1016/j.jhepr.2024.101223 · Cited by 35 · see citing works
Self-citationSiapoush, S., Rezaei, R., Alavifard, H., Hatami, B., Zali, M. R., Vosough, M., Lorzadeh, S., Łoś, M., Baghaei, K., & Ghavami, S. (2023) · Life Sciences · doi:10.1016/j.lfs.2023.121894 · Cited by 32 · see citing works
Boo, Y. C. (2024) · Antioxidants · doi:10.3390/antiox13020146 · Cited by 25 · see citing works
Zhang, M., Wu, Z., Salas, S. S., Martínez-Aguilar, M., Almanza, M. C. T., Buist‐Homan, M., & Moshage, H. J. (2023) · Journal of Cellular Biochemistry · doi:10.1002/jcb.30403 · Cited by 24 · see citing works
Improved Precision-Cut Liver Slice Cultures for Testing Drug-Induced Liver Fibrosis
Dewyse, L., Smet, V. D., Verhulst, S., Eysackers, N., Kunda, R., Messaoudi, N., Reynaert, H., & van Grunsven, L. A. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2022.862185 · Cited by 23 · see citing works
Self-citationEzhilarasan, D., & Najimi, M. (2022) · Journal of Cellular Physiology · doi:10.1002/jcp.30915 · Cited by 22 · see citing works
Aghara, H., Chadha, P., Zala, D., & Mandal, P. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1205821 · Cited by 19 · see citing works
ScRNA-seq and ST-seq in liver research
He, J., Deng, C., Krall, L., & Shan, Z. (2023) · Cell Regeneration · doi:10.1186/s13619-022-00152-5 · Cited by 17 · see citing works
Le, T. V., Phan-Thi, H.-T., Huynh-Thi, M.-X., Dang, T. M., Holterman, A., Grassi, G., Uyen, N. L. T., & Truong, N. H. (2023) · Cells · doi:10.3390/cells12071025 · Cited by 18 · see citing works
Zhao, W., Yuan, W., Dong, T., Qi, W., Feng, Z., Li, C., & Sun, Y. (2025) · Communications Biology · doi:10.1038/s42003-025-08160-2 · Cited by 16 · see citing works
Sani, F., Zomorrod, M. S., Azarpira, N., & Soleimani, M. (2023) · Stem Cells International · doi:10.1155/2023/8836452 · Cited by 17 · see citing works
RetractedAzizsoltani, A., Niknam, B., Taghizadeh-Teymorloei, M., Ghoodjani, E., Dianat-Moghadam, H., & Alizadeh, E. (2025) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2025.118249 · Cited by 15 · see citing works
Interplay between YAP/TAZ and metabolic dysfunction-associated steatotic liver disease progression
Lee, N. Y., Choi, M. G., Lee, E. J., & Koo, J. H. (2024) · Archives of Pharmacal Research · doi:10.1007/s12272-024-01501-5 · Cited by 16 · see citing works
Xie, S., Xu, J., Chen, L., Qi, Y. M., Yang, H., & Tan, B. (2024) · Journal of Nutrition · doi:10.1016/j.tjnut.2024.06.016 · Cited by 15 · see citing works
Chen, L., Huang, Y., Zhang, N., Qu, J., Fang, Y., Fu, J., Yuan, Y., Zhang, Q., Li, H., Wen, Z., et al. (2024) · MedComm · doi:10.1002/mco2.654 · Cited by 15 · see citing works
Exploring Extracellular Vesicles: A Novel Approach in Nonalcoholic Fatty Liver Disease
Medina, A. I. V., Shi, M., Wang, Y., Wang, Z., Huang, K., & Liu, Y. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c09209 · Cited by 15 · see citing works
Mohammed, O. A. (2023) · Pathology - Research and Practice · doi:10.1016/j.prp.2023.154833 · Cited by 14 · see citing works
Paluschinski, M., Kordes, C., Vucur, M., Büttner, V., Roderburg, C., Xu, H., Shinte, P. V., Lang, P. A., Luedde, T., & Castoldi, M. (2023) · Cells · doi:10.3390/cells12151955 · Cited by 11 · see citing works
Zhang, C., Liu, S., Sui, Y., & Yang, M. J. (2025) · World Journal of Hepatology · doi:10.4254/wjh.v17.i11.113756 · Cited by 10 · see citing works
Zhang, M., Jing, Y., Xu, W., Shi, X., Zhang, W., Chen, P., Cao, X., Han, X., Duan, X., & Ren, J. (2023) · Cell Death and Disease · doi:10.1038/s41419-023-06324-8 · Cited by 10 · see citing works
O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in myofibroblasts
Very, N., Boulet, C., Gheeraert, C., Berthier, A., Johanns, M., Saleh, M. B., Guille, L., Bray, F., Strub, J., Bobowski, M., et al. (2024) · Cell Death and Disease · doi:10.1038/s41419-024-06773-9 · Cited by 10 · see citing works
Cysteine sulfenylation contributes to liver fibrosis via the regulation of EphB2-mediated signaling
Han, Y., Gao, Q., Xu, Y., Chen, K., Li, R., Guo, W., & Wang, S. (2024) · Cell Death and Disease · doi:10.1038/s41419-024-06997-9 · Cited by 10 · see citing works
Hoebinger, C., Rajcic, D., Silva, B., & Hendrikx, T. (2023) · Frontiers in Endocrinology · doi:10.3389/fendo.2023.1148827 · Cited by 9 · see citing works
Danoy, M., Jellali, R., Tauran, Y., Bruce, J., Leduc, M., Gilard, F., Gakière, B., Scheidecker, B., Kido, T., Miyajima, A., et al. (2021) · Differentiation · doi:10.1016/j.diff.2021.06.001 · Cited by 9 · see citing works
Nuclear Pirin promotes HCC by acting as a key inflammation-facilitating factor
Ma, H., Cao, T., Zhang, F., Sun, D., Chen, L., Lin, Y., Lai, S., Jiang, B., Zhou, Y., You, J. A., et al. (2025) · Gut · doi:10.1136/gutjnl-2024-334087 · Cited by 9 · see citing works
Zhang, B., Liu, M., Jiang, L., Chen, Z., Tian, W., Li, K., Yu, B., Zhang, W., Li, S., Zhou, Y., et al. (2025) · Current Microbiology · doi:10.1007/s00284-025-04474-w · Cited by 8 · see citing works
Dai, X., Zheng, H.-L., Ma, Y.-X., Wang, Y., Wang, M.-Q., Cai, H., Hu, Z., Wan, J., & Zhang, L. (2024) · Frontiers in Bioscience-Landmark · doi:10.31083/j.fbl2902062 · Cited by 8 · see citing works
Yan, X., Han, Q., Wang, Y., Li, W., Zhang, N., Xu, F., Chen, W., You, H., & Yang, A. (2025) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2025.101637 · Cited by 7 · see citing works
ADAMTS12 is a stromal modulator in chronic liver disease
Dekky, B., Azar, F., Bonnier, D., Monseur, C., Kalebić, C., Arpigny, E., Colige, A., Legagneux, V., & Théret, N. (2023) · The FASEB Journal · doi:10.1096/fj.202200692rrrr · Cited by 7 · see citing works
Han, H., Yu, D., Liu, Y., Jia, H., & Liu, W. (2025) · Science Advances · doi:10.1126/sciadv.adz4126 · Cited by 7 · see citing works
Zhang, Q., Wang, L., Li, H., Wu, F., Tang, Z., Li, H., Wang, C., & Zhao, X. (2024) · Food Reviews International · doi:10.1080/87559129.2024.2396841 · Cited by 6 · see citing works
Nooreen, Z., Wal, A., Shukla, A., Badarinath, A. V., Kumar, N., Sarma, T., Khan, A., & Gasmi, A. (2025) · Letters in Drug Design & Discovery · doi:10.1016/j.lddd.2025.100268 · Cited by 6 · see citing works
Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Molecular Medicine · doi:10.1186/s10020-024-00871-2 · Cited by 6 · see citing works
Sharma, N., Chandra, Y., & Andugulapati, S. B. (2025) · Liver International · doi:10.1111/liv.70050 · Cited by 5 · see citing works
Orphan receptor GPR176 in hepatic stellate cells exerts a profibrotic role in chronic liver disease
Smet, V. D., Gürbüz, E., Eysackers, N., Dewyse, L., Smout, A., Dastjerd, M. K., Lefesvre, P., Messaoudi, N., Reynaert, H., Verhulst, S., et al. (2024) · JHEP Reports · doi:10.1016/j.jhepr.2024.101036 · Cited by 5 · see citing works
Self-citationLiver Progenitor Cells: Cellular Origins, Plasticity, and Signaling Pathways in Liver Regeneration
Han, J., Sung, A., Jeong, H., & Jung, Y. (2025) · Biology · doi:10.3390/biology14101361 · Cited by 5 · see citing works
Mijit, A., Zhang, Y., Wu, Y., Li, M., Wang, Z., & Cheng, L.-F. (2025) · Biochemical Pharmacology · doi:10.1016/j.bcp.2025.117120 · Cited by 4 · see citing works
Li, X., Xiang, X., Jiao, Q., Luo, Y., Zeng, G., Zhang, W., Qin, Y., & Chen, Y. (2025) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2025.125403 · Cited by 4 · see citing works
Identification of novel gene expression programs in thrombin-stimulated hepatic stellate cells
Mac, N. A., Prasad, S., Capece, G. E., Martínez‐Zamudio, R. I., McClenaghan, C., & Poole, L. G. (2025) · Research and Practice in Thrombosis and Haemostasis · doi:10.1016/j.rpth.2025.103223 · Cited by 3 · see citing works
Han, L., Lu, F., Chen, S.-J., Zhang, Q.-X., Wu, H.-Y., Huang, T., Pu, H.-F., Wang, J.-L., Zou, G.-L., Pan, C., et al. (2025) · Human Mutation · doi:10.1155/humu/1584910 · Cited by 3 · see citing works
Feng, X., Feng, Q., Liu, S., Zhang, L., Raza, S. H. A., Cai, B., Ma, Y., Li, F., & Ma, Y. (2026) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01803-x · Cited by 3 · see citing works
Global molecular landscape of early MASLD progression in human obesity
Zhao, Q., Nardo, W. D., Wang, R., Zhong, Y., Keles, U., Sakalauskaite, G., Zhao, L. N., Tay, H., Youhanna, S. C., Yan, M., et al. (2026) · eLife · doi:10.1101/2025.01.13.632747 · Cited by 3 · see citing works
Wang, P., Pan, J., Gong, S., Zhang, Z., & Li, B. (2024) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2024.111139 · Cited by 3 · see citing works
Poly(2-oxazoline) Homopolymers and Diblock Copolymers Containing Retinoate ω-End Groups
Stafast, L. M., Weber, C., Kuchenbrod, M. T., Hoeppener, S., Behnke, M., Schubert, S., Mehmetaj, K., Press, A. T., Bauer, M. W., & Schubert, U. S. (2022) · ACS Applied Polymer Materials · doi:10.1021/acsapm.2c00037 · Cited by 3 · see citing works
An immunocompetent murine model of virus-elicited liver fibrosis and hepatocellular carcinoma
Batista, M. N., Bordignon, J., Mosimann, A. L. P., Bobrowski, T., Chen, H.-A., Tobin-Xet, G., Barrall, E. A., Prokhnevska, N., Vaidya, A. B., Lewy, T., et al. (2026) · Journal of Hepatology · doi:10.1016/j.jhep.2026.02.020 · Cited by 2 · see citing works
Saha, T., Mairal, A., Mehrotra, S., Sarin, S. K., & Kumar, A. (2026) · Advanced Science · doi:10.1002/advs.76420 · Cited by 2 · see citing works
Generation and Culture of Primary Mouse Hepatocyte–Hepatic Stellate Cell Spheroids
Mannaerts, I., Eysackers, N., & van Grunsven, L. A. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3207-9_11 · Cited by 2 · see citing works
Self-citationGlobal molecular landscape of early MASLD progression in human obesityPreprint
Zhao, Q., Nardo, W. D., Wang, R., Zhong, Y., Keles, U., Sakalauskaite, G., Zhao, L. N., Tay, H., Youhanna, S. C., Yan, M., et al. (2025) · eLife · doi:10.7554/elife.109534 · Cited by 2 · see citing works
Sanz‐García, C., Nevzorova, Y. A., Martı́nez-Naves, E., Cubero, F. J., Gutierrez, A. H., Sañudo, J. R., Enrich, C., Rentero, C., Sancho‐Bru, P., Macías‐Rodríguez, R. U., et al. (2022) · Gastroenterología y Hepatología · doi:10.1016/j.gastrohep.2022.05.012 · Cited by 1 · see citing works
Recent advances in multicellular human liver models
Mun, S. J., Lee, J., Shin, Y., Eun, V., Ji, Y., & Son, M. J. (2022) · Organoid · doi:10.51335/organoid.2022.2.e26 · Cited by 1 · see citing works
Caon, E., Forlano, R., Mullish, B. H., Manousou, P., & Rombouts, K. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95262-0.00025-5 · Cited by 1 · see citing works
Liao, J., Yuan, Q., Luo, L., Hu, X., Li, Z., & Zhang, Z. (2024) · Journal of Cell Communication and Signaling · doi:10.1002/ccs3.12033 · Cited by 1 · see citing works
Virzì, A., Boulahtouf, Z., Moehlin, J., Girard, L., Heydmann, L., Bach, C., Gerges, E., Durand, S., Oudot, M. A., Suarez, A. A. R., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-09870-7 · Cited by 1 · see citing works
(290115), Y. L., Liu, M.-Q., Wang, J., Li, Z., (91924), X. W., Yan-bo, W., Zhang, N., Dong, L., & An, W. (2026) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2026.06.010 · Cited by 1 · see citing works
Pujie, L. I. U., Long, K., Wang, Y., Sulaiman, J. E., Wang, B., Xiangyu, Z. H. O. U., Liu, C., CHENG, Y., Cai, Y., Li, H., et al. (2026) · Metabolism · doi:10.1016/j.metabol.2026.156726 · Cited by 1 · see citing works
Tsouri, E., Athanasopoulou, N., Akkari, L., & Vegna, S. (2026) · Seminars in Immunology · doi:10.1016/j.smim.2026.102053 · Cited by 1 · see citing works
Sanz‐García, C., Nevzorova, Y. A., Martı́nez-Naves, E., Cubero, F. J., Gutierrez, A. H., Sañudo, J. R., Enrich, C., Rentero, C., Sancho‐Bru, P., Macías‐Rodríguez, R. U., et al. (2023) · Gastroenterología y Hepatología (English Edition) · doi:10.1016/j.gastre.2022.05.010 · Cited by 1 · see citing works
Overview of the Therapeutic Mechanism of Liver Fibrosis Based on Hepatic Stellate Cells
赵, 思. (2022) · Advances in Clinical Medicine · doi:10.12677/acm.2022.125698
Liang, M.-X., Zhou, Y., Li, S., Xiang, W.-S., Pan, Z.-Q., Chen, Y.-H., & He, Y.-H. (2025) · World Journal of Gastroenterology · doi:10.3748/wjg.v31.i14.104523
Wang, J., Tao, J., Xia, C., Tang, M., Stoica, A.-F., Yuan, S., Li, Y., Guo, L., Kong, X., & Wang, J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.25.690307
Bhattacharjee, J., Wang, L. X., Meneses, B., Emamaullee, J. A., Frey, M. R., & Kohli, R. (2025) · Gastro Hep Advances · doi:10.1016/j.gastha.2025.100854
Global molecular landscape of early MASLD progression in obesityPreprint
Zhao, Q., Nardo, W. D., Wang, R., Zhong, Y., Keles, U., Sakalauskaite, G., Zhao, L. N., Tay, H., Youhanna, S. C., Yan, M., et al. (2025) · eLife · doi:10.7554/elife.109534.1
Additional file 12 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758598
Additional file 8 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758586
Additional file 5 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758577
Additional file 7 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758583
Additional file 1 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758565
Additional file 11 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758595
Additional file 4 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758574
Additional file 2 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758568
Additional file 6 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758580
Additional file 9 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758589
Additional file 10 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758592
Additional file 3 of Stellate cells are in utero markers of pancreatic disease in cystic fibrosis
Leir, S.-H., Tkachenko, S., Paranjapye, A., Meckler, F., Wettere, A. J. V., Kerschner, J. L., Kuznetsov, E., Schacht, M., Gillurkar, P., Regouski, M., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26758571
Ramírez-Mejía, M. M., Ponciano‐Rodríguez, G., Poo, J. L., & Méndez‐Sánchez, N. (2026) · Archives of Medical Research · doi:10.1016/j.arcmed.2026.103387
Global molecular landscape of early MASLD progression in obesityPreprint
Zhao, Q., Nardo, W. D., Wang, R., Zhong, Y., Keles, U., Sakalauskaite, G., Zhao, L. N., Tay, H., Youhanna, S. C., Yan, M., et al. (2026) · eLife · doi:10.7554/elife.109534.2
Feng, X., Feng, Y., Raza, S. H. A., Ma, Y. P., & Deng, H. (2026) · Frontiers in Genetics · doi:10.3389/fgene.2026.1781941 · Cited by 1 · see citing works
Martinez, M. B., Filomeni, G., & Rizza, S. (2026) · STAR Protocols · doi:10.1016/j.xpro.2026.104639
Naeem, M. Y., & Ijaz, T. (2026) · Food Science and Biotechnology · doi:10.1007/s10068-026-02236-3
Multimodal assessment of liver stiffness and portal hemodynamics in a cirrhotic mouse model
Zhang, J., Huo, Y., Bian, J., Cha, L., Yang, Q., Zhao, C., Li, J., Zhao, D., Feng, D., & Gao, L. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1888436
Liu, X., Li, Y.-X., Guan, J.-K., Fan, L., Li, S.-Q., Hassan, W., Ahmed, H., Cheng, L., & Wang, Y. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1810588
Matrix stiffness shapes early transcriptional trajectories during hepatic stellate cell activation
Inada, K., Miyoshi, M., Kakinuma, S., Watakabe, K., Mochida, T., Shimizu, T., Tsuchiya, J., Nobusawa, T., Kaneko, S., Kawai‐Kitahata, F., et al. (2026) · Hepatology International · doi:10.1007/s12072-026-11141-0
- Authors
- De Smet, V., Eysackers, N., Merens, V., Kazemzadeh Dastjerd, M., Halder, G., Verhulst, S., Mannaerts, I., & van Grunsven, L. A.
- Affiliations
- Vrije Universiteit Brussel (BE)
- VIB-KU Leuven Center for Cancer Biology (BE)
- ORCID
- Venue
- Cell Death & Disease
- Published
- 2021-11-27
- Volume
- 12
- Issue
- 12
- Pages
- 1110-1110
- ISSN
- 2041-4889 (ISSN-L)
- PMC
- PMC8627507
- OpenAlex
- W3216990250
- Cited by
- 78
- References
- 36
- Citations by year
- 2026: 15 · 2025: 24 · 2024: 17 · 2023: 15 · 2022: 6 · 2021: 1
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Cell Death and Disease (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lirias (KU Leuven) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- Funders
- Fonds Wetenschappelijk Onderzoek (1192920N, 1243121N, 117300 / 12N5415N LV)
- Bijzonder Onderzoeksfonds
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Health Sciences › Medicine › Hepatology › Liver Diseases and Immunity
- Keywords
- hepatic stellate cells, chronic liver disease, liver fibrosis, extracellular matrix, transcriptional programs, liver cirrhosis, single-cell RNA sequencing, druggable targets
- MeSH terms
- Animals, Chronic Disease, Disease Models, Animal, Humans, Liver Diseases, Male, Mice, Hepatic Stellate Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
