The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Optical imaging of metabolic dynamics in animals
Open accessAbstract
Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D 2 O) probing with stimulated Raman scattering (DO-SRS) microscopy to image in situ metabolic activities. Enzymatic incorporation of D 2 O-derived deuterium into macromolecules generates carbon–deuterium (C–D) bonds, which track biosynthesis in tissues and can be imaged by SRS in situ. Within the broad vibrational spectra of C–D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C–D signals with macromolecular selectivity. DO-SRS microscopy enables us to probe de novo lipogenesis in animals, image protein biosynthesis without tissue bias, and simultaneously visualize lipid and protein metabolism and reveal their different dynamics. DO-SRS microscopy, being noninvasive, universally applicable, and cost-effective, can be adapted to a broad range of biological systems to study development, tissue homeostasis, aging, and tumor heterogeneity.
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Self-citationStochastic and genetic factors influence tissue-specific decline in ageing C. elegans
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Biological imaging of chemical bonds by stimulated Raman scattering microscopy
Hu, F., Shi, L., & Min, W. (2019) · Nature Methods · doi:10.1038/s41592-019-0538-0 · Cited by 392
Self-citationSchiessl, K. T., Hu, F., Jo, J., Nazia, S. Z., Wang, B., Price-Whelan, A., Min, W., & Dietrich, L. E. P. (2019) · Nature Communications · doi:10.1038/s41467-019-08733-w · Cited by 258
Self-citationMicroglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK
Li, Y., Munoz-Mayorga, D., Nie, Y., Kang, N., Tao, Y., Lagerwall, J., Pernaci, C., Curtin, G., Coufal, N. G., Mertens, J., et al. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.03.014 · Cited by 160
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Metadata
- Authors
- Shi, L., Zheng, C., Shen, Y., Chen, Z., Silveira, E. S., Zhang, L., Wei, M., Liu, C., de Sena-Tomas, C., Targoff, K., & Min, W.
- Venue
- Nature Communications
- Published
- 2018-08-06
- Volume
- 9
- Issue
- 1
- Pages
- 2995-2995
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2952902070
- Cited by
- 264
- References
- 60
- FWCI
- 8.48
- Type
- journal article
- Language
- en
- Open access
- gold
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- Shi, L., Zheng, C., Shen, Y., Chen, Z., Silveira, E. S., Zhang, L., Wei, M., Liu, C., de Sena-Tomas, C., Targoff, K., & Min, W. (2018). Optical imaging of metabolic dynamics in animals. Nature Communications. https://doi.org/10.1038/s41467-018-05401-3
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