CoChat
Sign Up
Cited by 124Signaling Pathways in DiseaseAntifungal resistance and susceptibilityMicrobial Natural Products and Biosynthesis

The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.

Harnessing calcineurin-FK506-FKBP12 crystal structures from invasive fungal pathogens to develop antifungal agents

Open access
Juvvadi, Praveen R., Fox, David, Bobay, Benjamin G., Hoy, Michael J., Gobeil, Sophie M. C., Venters, Ronald A., Chang, Zanetta, Lin, Jackie J., Averette, Anna Floyd, Cole, D. Christopher, Barrington, Blake C., Wheaton, Joshua D., Ciofani, Maria, Trzoss, Michael, Li, Xiaoming, Lee, Soo Chan, Chen, Ying-Lien, Mutz, Mitchell, Spicer, Leonard D., Schumacher, Maria A., Heitman, Joseph, Steinbach, William J.·2019·Nature Communications
DOI 10.1038/s41467-019-12199-1PMC PMC6753081
Open this paper in CoChat — free accountOpen PDF

Abstract

Calcineurin is important for fungal virulence and a potential antifungal target, but compounds targeting calcineurin, such as FK506, are immunosuppressive. Here we report the crystal structures of calcineurin catalytic (CnA) and regulatory (CnB) subunits complexed with FK506 and the FK506-binding protein (FKBP12) from human fungal pathogens (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans and Coccidioides immitis). Fungal calcineurin complexes are similar to the mammalian complex, but comparison of fungal and human FKBP12 (hFKBP12) reveals conformational differences in the 40s and 80s loops. NMR analysis, molecular dynamic simulations, and mutations of the A. fumigatus CnA/CnB-FK506-FKBP12-complex identify a Phe88 residue, not conserved in hFKBP12, as critical for binding and inhibition of fungal calcineurin. These differences enable us to develop a less immunosuppressive FK506 analog, APX879, with an acetohydrazine substitution of the C22-carbonyl of FK506. APX879 exhibits reduced immunosuppressive activity and retains broad-spectrum antifungal activity and efficacy in a murine model of invasive fungal infection.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41467-019-12199-1

Cites

57 of 57 references verified0 citation errors · 0 not checkable

Statistics

Figures

Fig. 1 Crystal structures of fungal CN–FK506-FKBP12 complexes. a Overlay of fungal CNs with the bovine complex (bovine: PDB 1TCO gray; fungal: CnA red, CnB blue, FKBP12 green and FK506 in orange). b RMSD of fungal CN complexes to the bovine complex and isolated components with Homo sapiens CN complex (CnA/CnB; PDB 4OR9) and H. sapiens FKBP12 (PDB 2PPN) in parentheses. c Alignment of human and fungal FKBP12s. Residues within 5 Å of FK506 are highlighted yellow. Non-conserved residues in fungi are highlighted green. d Structural comparison of variable residues in A. fumigatus (colored as in a; PDB 6TZ7) and H. sapiens FKBP12 (colored yellow; PDB 2PPN). Note that both AfFKBP12 F22 and Q50 reside distal (>10 Å) to the FKBP12-FK506-binding site on CnA or CnB (panel 1). The side-chains of AfFKBP12 R55 and hFKBP12 E55 are near the BBH and CnB, but face away from FK506 and do not make conserved contacts to CnA/CnB other than a distal main-chain carbonyl interaction with FK506 (panel 3). See also Supplementary Fig. 1 and Supplementary Tables 1 and 2
Fig. 1 Crystal structures of fungal CN–FK506-FKBP12 complexes. a Overlay of fungal CNs with the bovine complex (bovine: PDB 1TCO gray; fungal: CnA red, CnB blue, FKBP12 green and FK506 in orange). b RMSD of fungal CN complexes to the bovine complex and isolated components with Homo sapiens CN complex (CnA/CnB; PDB 4OR9) and H. sapiens FKBP12 (PDB 2PPN) in parentheses. c Alignment of human and fungal FKBP12s. Residues within 5 Å of FK506 are highlighted yellow. Non-conserved residues in fungi are highlighted green. d Structural comparison of variable residues in A. fumigatus (colored as in a; PDB 6TZ7) and H. sapiens FKBP12 (colored yellow; PDB 2PPN). Note that both AfFKBP12 F22 and Q50 reside distal (>10 Å) to the FKBP12-FK506-binding site on CnA or CnB (panel 1). The side-chains of AfFKBP12 R55 and hFKBP12 E55 are near the BBH and CnB, but face away from FK506 and do not make conserved contacts to CnA/CnB other than a distal main-chain carbonyl interaction with FK506 (panel 3). See also Supplementary Fig. 1 and Supplementary Tables 1 and 2CC BY · PubMed Central
Fig. 2 Structural implication of AfFKBP12 mutations. a NMR analysis showing chemical shifts induced by FK506 binding to A. fumigatus (red) and human (black) FKBP12. b Growth of the Affkbp12 mutants in the absence and presence of FK506 for 5 days at 37 °C. c Microscopic localization AfFKBP12 mutated proteins in vivo in the absence or presence of FK506. Arrows show nuclear localization of AfFKBP12. Arrowheads indicate binding of AfFKBP12 to CN at the hyphal septum (d F22T, e Q50M, f R55E, g F88H). The FK506-binding pocket of WT (blue surface) and the pocket resulting from the mutation (red surface) are shown. Mutation site is denoted with an “*” and shown in stick format (red bar in bar graph), while residues comprising the binding pocket are shown in orange lines (black bars in bar graph). The stability and quality of the simulation is shown in the Cα RMSD plot. h The all atom (top) and backbone (bottom) RMSD denoting the structural variation due to the mutation compared to the WT structure (5HWB). See also Supplementary Figs. 1 and 3
Fig. 2 Structural implication of AfFKBP12 mutations. a NMR analysis showing chemical shifts induced by FK506 binding to A. fumigatus (red) and human (black) FKBP12. b Growth of the Affkbp12 mutants in the absence and presence of FK506 for 5 days at 37 °C. c Microscopic localization AfFKBP12 mutated proteins in vivo in the absence or presence of FK506. Arrows show nuclear localization of AfFKBP12. Arrowheads indicate binding of AfFKBP12 to CN at the hyphal septum (d F22T, e Q50M, f R55E, g F88H). The FK506-binding pocket of WT (blue surface) and the pocket resulting from the mutation (red surface) are shown. Mutation site is denoted with an “*” and shown in stick format (red bar in bar graph), while residues comprising the binding pocket are shown in orange lines (black bars in bar graph). The stability and quality of the simulation is shown in the Cα RMSD plot. h The all atom (top) and backbone (bottom) RMSD denoting the structural variation due to the mutation compared to the WT structure (5HWB). See also Supplementary Figs. 1 and 3CC BY · PubMed Central
Fig. 3 X-ray and MD-simulated structures of A. fumigatus and human CN-complex. a Conformational rotation of FKBP12 about the FK506-binding pocket comparing the X-ray characterized and MD characterized CnA (red)/CnB(blue)–FKBP12 [olive (X-ray) and green (MD simulated)]–FK506 complex. b Conformational rotation of FKBP12 about the FK506-binding pocket comparing the MD characterized A. fumigatus complex and the MD characterized human CnA (red)/CnB(blue)–FKBP12 [green (A. fumigatus) and yellow (human)]–FK506 complex. Rotation in a and b are depicted by dashed arrow lines color coded based on the FKBP12 coloring in each panel. c Growth of the A. fumigatus strain expressing codon-optimized hFKBP12 in the absence and presence of FK506 for 5 days at 37 °C. d Microscopic localization hFKBP12-GFP in A. fumigatus in the absence or presence of FK506. Arrows show nuclear localization of hFKBP12. Arrowheads indicate the binding of hFKBP12 to CN at the hyphal septum. e Center of mass (COM) distances between different chains convey the stability of the complexes over the last 100 ns; top—CnA to FKBP12, middle—CnA to FK506, and bottom—FKBP12 to FK506. See also Supplementary Figs. 4 and 5
Fig. 3 X-ray and MD-simulated structures of A. fumigatus and human CN-complex. a Conformational rotation of FKBP12 about the FK506-binding pocket comparing the X-ray characterized and MD characterized CnA (red)/CnB(blue)–FKBP12 [olive (X-ray) and green (MD simulated)]–FK506 complex. b Conformational rotation of FKBP12 about the FK506-binding pocket comparing the MD characterized A. fumigatus complex and the MD characterized human CnA (red)/CnB(blue)–FKBP12 [green (A. fumigatus) and yellow (human)]–FK506 complex. Rotation in a and b are depicted by dashed arrow lines color coded based on the FKBP12 coloring in each panel. c Growth of the A. fumigatus strain expressing codon-optimized hFKBP12 in the absence and presence of FK506 for 5 days at 37 °C. d Microscopic localization hFKBP12-GFP in A. fumigatus in the absence or presence of FK506. Arrows show nuclear localization of hFKBP12. Arrowheads indicate the binding of hFKBP12 to CN at the hyphal septum. e Center of mass (COM) distances between different chains convey the stability of the complexes over the last 100 ns; top—CnA to FKBP12, middle—CnA to FK506, and bottom—FKBP12 to FK506. See also Supplementary Figs. 4 and 5CC BY · PubMed Central
Fig. 4 FK506 susceptibility and structural implication of hFKBP12 mutants. a Growth of the hFKBP12 mutants in the absence and presence of FK506 for 3 days at 37 °C (b K48Q, c Q50M, d K48Q–Q50M, e H88F) The FK506-binding pocket of WT (blue surface) and the pocket resulting from the mutation (red surface) are shown. Mutation site is denoted with a “*” and shown in stick format (red bar in bar graph), while residues comprising the binding pocket are shown in orange lines (black bars in bar graph). Stability and quality of the simulation is shown in the Cα RMSD plot. f All atom (top) and backbone (bottom) RMSD denoting the structural variation due to the mutation compared to the WT structure (1FKF-monomer). See also Supplementary Figs. 6 and 11
Fig. 4 FK506 susceptibility and structural implication of hFKBP12 mutants. a Growth of the hFKBP12 mutants in the absence and presence of FK506 for 3 days at 37 °C (b K48Q, c Q50M, d K48Q–Q50M, e H88F) The FK506-binding pocket of WT (blue surface) and the pocket resulting from the mutation (red surface) are shown. Mutation site is denoted with a “*” and shown in stick format (red bar in bar graph), while residues comprising the binding pocket are shown in orange lines (black bars in bar graph). Stability and quality of the simulation is shown in the Cα RMSD plot. f All atom (top) and backbone (bottom) RMSD denoting the structural variation due to the mutation compared to the WT structure (1FKF-monomer). See also Supplementary Figs. 6 and 11CC BY · PubMed Central
Fig. 5 Binding of FKBP12–FK506 to A. fumigatus CN. a Cartoon representation of A. fumigatus WT AfFKBP12–FK506 complex binding to A. fumigatus CN (CnA/CnB) from the MD simulation. Cartoon representations colored as follows: CnA (red), CnB (blue), AfFKBP12 (green), and FK506 (orange sticks and spheres). b Cartoon representation of Human hFKBP12:FK506 complex binding to A. fumigatus CnA/CnB from the MD simulation. Cartoon representations colored as follows: CnA (red), CnB (blue), hFKBP12 (yellow), and FK506 (orange sticks and spheres). Residue labels are shown for clarity and colored to the same color as their protein. Residues (those with more than 2000 contacts in the last 100 ns of the simulation) depicted as lines are contacts between either FKBP12 and CnA or CnB, while sticks are contacts between FK506 and CnA or CnB. Valence states are shown for label clarity. Bar graphs denote all contacts within 6 Å during the last 100 ns of each simulation; top bar graph, FKBP12 contacts to CnA (black) and CnB (red); middle graph, CnA contacts to FKBP12 (black), and FK506 (red), slanted lines denote residues 201–324 to which there are no contacts in CnA; bottom graph, CnB contacts to FKBP12 (black), and FK506 (red). See also Supplementary Figs. 7 and 8 and Supplementary Table 2
Fig. 5 Binding of FKBP12–FK506 to A. fumigatus CN. a Cartoon representation of A. fumigatus WT AfFKBP12–FK506 complex binding to A. fumigatus CN (CnA/CnB) from the MD simulation. Cartoon representations colored as follows: CnA (red), CnB (blue), AfFKBP12 (green), and FK506 (orange sticks and spheres). b Cartoon representation of Human hFKBP12:FK506 complex binding to A. fumigatus CnA/CnB from the MD simulation. Cartoon representations colored as follows: CnA (red), CnB (blue), hFKBP12 (yellow), and FK506 (orange sticks and spheres). Residue labels are shown for clarity and colored to the same color as their protein. Residues (those with more than 2000 contacts in the last 100 ns of the simulation) depicted as lines are contacts between either FKBP12 and CnA or CnB, while sticks are contacts between FK506 and CnA or CnB. Valence states are shown for label clarity. Bar graphs denote all contacts within 6 Å during the last 100 ns of each simulation; top bar graph, FKBP12 contacts to CnA (black) and CnB (red); middle graph, CnA contacts to FKBP12 (black), and FK506 (red), slanted lines denote residues 201–324 to which there are no contacts in CnA; bottom graph, CnB contacts to FKBP12 (black), and FK506 (red). See also Supplementary Figs. 7 and 8 and Supplementary Table 2CC BY · PubMed Central
Fig. 6 The FK506 analog APX879 has reduced immunosuppressive activity. a Chemical structures of FK506 (left) and APX879 (right) with modified C22 indicated in blue. b Model of APX879 bound to A. fumigatus CN–FKBP12 complex. APX879 was overlaid on FK506 in the A. fumigatus crystal structure and steric clashes with the acetohydrazine substitution were minimized manually. The oxygen atom donated by the substitution was placed such that it occupies a similar space to the conserved water bound to the BBH. c Naive primary murine CD4+ T cells (from C57BL/6 mice) activated and differentiated in vitro and treated with FK506 have a reduced fraction of IL-2-producing cells than those treated with APX879. Dose response curves demonstrate an over 70-fold decrease in IL-2 production for FK506 compared to APX879. Note that the plot is showing a 95% confidence interval for the four-parameter curve fit, error bars for individual points represent the standard error value. The IC50 range is 0.17–0.21 for FK506 and 10.7–16.9 for APX879. d, e T helper cells (d) and germinal center (GC) B cells (e) collected from lymph nodes of mice treated for 8 days with vehicle, 5 mg/kg FK506, or 20 mg/kg APX879. Lymph nodes were collected 7 days following immunization with NP-OVA to stimulate T cell-dependent GC-B cell response. Error bars indicate boot strapped 95% confidence intervals. See also Supplementary Fig. 9
Fig. 6 The FK506 analog APX879 has reduced immunosuppressive activity. a Chemical structures of FK506 (left) and APX879 (right) with modified C22 indicated in blue. b Model of APX879 bound to A. fumigatus CN–FKBP12 complex. APX879 was overlaid on FK506 in the A. fumigatus crystal structure and steric clashes with the acetohydrazine substitution were minimized manually. The oxygen atom donated by the substitution was placed such that it occupies a similar space to the conserved water bound to the BBH. c Naive primary murine CD4+ T cells (from C57BL/6 mice) activated and differentiated in vitro and treated with FK506 have a reduced fraction of IL-2-producing cells than those treated with APX879. Dose response curves demonstrate an over 70-fold decrease in IL-2 production for FK506 compared to APX879. Note that the plot is showing a 95% confidence interval for the four-parameter curve fit, error bars for individual points represent the standard error value. The IC50 range is 0.17–0.21 for FK506 and 10.7–16.9 for APX879. d, e T helper cells (d) and germinal center (GC) B cells (e) collected from lymph nodes of mice treated for 8 days with vehicle, 5 mg/kg FK506, or 20 mg/kg APX879. Lymph nodes were collected 7 days following immunization with NP-OVA to stimulate T cell-dependent GC-B cell response. Error bars indicate boot strapped 95% confidence intervals. See also Supplementary Fig. 9CC BY · PubMed Central
Fig. 7 Efficacy of APX879 in cryptococcal model of murine invasive fungal infection. a, b Survival curve of mice infected intranasally with C. neoformans WT (H99) and treated daily with indicated antifungal drugs. Treatment was halted at day 14 and animals were monitored for survival. Survival curves analyzed using log-rank Mantel–Cox test. c Fungal burdens in brain, lung, and spleen of H99 infected and drug-treated animals. **P < 0.01, ***P < 0.001. Lower error bars that exceeded the y-axis limits were excluded. See also Supplementary Fig. 10
Fig. 7 Efficacy of APX879 in cryptococcal model of murine invasive fungal infection. a, b Survival curve of mice infected intranasally with C. neoformans WT (H99) and treated daily with indicated antifungal drugs. Treatment was halted at day 14 and animals were monitored for survival. Survival curves analyzed using log-rank Mantel–Cox test. c Fungal burdens in brain, lung, and spleen of H99 infected and drug-treated animals. **P < 0.01, ***P < 0.001. Lower error bars that exceeded the y-axis limits were excluded. See also Supplementary Fig. 10CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 56 references

Cited by

Sorted by citations

Items 101–124 of 124

Related

See all

Metadata

Authors
Juvvadi, P. R., Fox, D., Bobay, B. G., Hoy, M. J., Gobeil, S. M. C., Venters, R. A., Chang, Z., Lin, J. J., Averette, A. F., Cole, D. C., Barrington, B. C., Wheaton, J. D., Ciofani, M., Trzoss, M., Li, X., Lee, S. C., Chen, Y.-L., Mutz, M., Spicer, L. D., Schumacher, M. A., Heitman, J., & Steinbach, W. J.
Affiliations
  • Duke University (US)
  • Beryllium (United States) (US)
  • Duke Medical Center (US)
  • Amplyx Pharmaceuticals (United States) (US)
  • The University of Texas at San Antonio (US)
  • National Taiwan University (TW)
  • Genentech (US)
Corresponding author
  • Juvvadi, Praveen R.
  • Steinbach, William J.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2019-09-19
Volume
10
Issue
1
Pages
4275-4275
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-019-12199-1
PMC
PMC6753081
OpenAlex
W2973294764
Cited by
116
References
56
Citations by year
2026: 10 · 2025: 11 · 2024: 14 · 2023: 14 · 2022: 17 · 2021: 26 · 2020: 22 · 2019: 1
FWCI
5.96
Type
journal article
Language
en
Update notices
correction (2025-07-03)
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (R01 AI112595-04, P01 AI104533-04, R43AI098300, R43AI106235, HHSN272200700057, HHSN272201200025C, HHSN272201700059C)
  • Ministry of Trade, Industry and Energy (N0001720)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
Keywords
calcineurin, FK506, FKBP12, antifungal agents, fungal virulence, Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Coccidioides immitis, immunosuppressive activity, invasive fungal infections, crystal structure
MeSH terms
Animals, Antifungal Agents, Aspergillosis, Aspergillus fumigatus, Binding Sites, Candida albicans, Cells, Cultured, Coccidioides, Cryptococcosis, Cryptococcus neoformans, Female, Male, Mice, Inbred A, Mice, Inbred C57BL, Tacrolimus, Crystallography, X-Ray, Calcineurin, Tacrolimus Binding Protein 1A, Mice, Drug Discovery, Molecular Dynamics Simulation, Calcineurin Inhibitors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review