The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
Open accessAbstract
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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Double-slit photoelectron interference in strong-field ionization of the neon dimer
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DNA targeting specificity of RNA-guided Cas9 nucleases
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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
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CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
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RNA targeting with CRISPR–Cas13
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Choi, J.-H., Lim, J., Shin, M., Paek, S.-H., & Choi, J.-W. (2021) · Nano Letters · doi:10.1021/acs.nanolett.0c04303 · Cited by 382
Multiplex genome-editing technologies for revolutionizing plant biology and crop improvement
Abdelrahman, M., Wei, Z., Rohila, J. S., & Zhao, K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.721203 · Cited by 125
Biofunctionalization of Multiplexed Silicon Photonic Biosensors
Puumala, L. S., Grist, S. M., Morales, J. M., Bickford, J. R., Chrostowski, L., Shekhar, S., & Cheung, K. C. (2022) · Biosensors · doi:10.3390/bios13010053 · Cited by 55
Functional genomics and the future of iPSCs in disease modeling
Brooks, I. R., Garrone, C. M., Kerins, C., Kiar, C. S., Syntaka, S., Xu, J. Z., Spagnoli, F. M., & Watt, F. M. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.03.019 · Cited by 54
Metadata
- Authors
- McCarty, N. S., Graham, A. E., Studená, L., & Ledesma-Amaro, R.
- Venue
- Nature Communications
- Published
- 2020-03-09
- Volume
- 11
- Issue
- 1
- Pages
- 1281-1281
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3010291540
- Cited by
- 552
- References
- 27
- FWCI
- 18.81
- Type
- journal article
- Language
- en
- Open access
- gold
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