An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment
Open accessPublished version of a preprint
Abstract
The world is entering a new era of the COVID-19 pandemic in which there is an increasing call for reliable antibody testing. To support decision making on the deployment of serology for either population screening or diagnostics, we present a detailed comparison of serological COVID-19 assays. We show that among the selected assays there is a wide diversity in assay performance in different scenarios and when correlated to virus neutralizing antibodies. The Wantai ELISA detecting total immunoglobulins against the receptor binding domain of SARS CoV-2, has the best overall characteristics to detect functional antibodies in different stages and severity of disease, including the potential to set a cut-off indicating the presence of protective antibodies. The large variety of available serological assays requires proper assay validation before deciding on deployment of assays for specific applications.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A Wantai ELISA ratio of >10 has previously been shown to correspond to virus neutralization.
“Although the Wantai ELISA is a qualitative assay, a ratio of >10 has previously been shown to correspond to virus neutralization (44).”
From SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients (2021) · cites this paper as [44]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Antibody responses to SARS-CoV-2 in patients with COVID-19
Long, Q.-X., Liu, B.-Z., Deng, H.-J., Wu, G.-C., Deng, K., Chen, Y.-K., Liao, P., Qiu, J.-F., Lin, Y., Cai, X.-F., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0897-1 · Cited by 2,876 · see citing works
Projecting the transmission dynamics of SARS-CoV-2 through the postpandemic period
Kissler, S. M., Tedijanto, C., Goldstein, E., Grad, Y. H., & Lipsitch, M. (2020) · Science · doi:10.1126/science.abb5793 · Cited by 2,611 · see citing works
Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019
Zhao, J., Yuan, Q., Wang, H., Liu, W., Liao, X., Su, Y., Wang, X., Yuan, J., Li, T., Li, J., et al. (2020) · Clinical Infectious Diseases · doi:10.1093/cid/ciaa344 · Cited by 2,607 · see citing works
Okba, N. M. A., Müller, M. A., Li, W., Wang, C., GeurtsvanKessel, C. H., Corman, V. M., Lamers, M. M., Sikkema, R. S., de Bruin, E., Chandler, F. D., et al. (2020) · Emerging Infectious Diseases · doi:10.3201/eid2607.200841 · Cited by 1,614 · see citing works
Self-citationFlaxman, S., Mishra, S., Gandy, A., Unwin, H., Coupland, H., Mellan, T., Zhu, H., Berah, T., Eaton, J., Guzman, P. P., et al. (2020) · Spiral (Imperial College London) · doi:10.25561/77731 · Cited by 522 · see citing works
Evaluation of nine commercial SARS-CoV-2 immunoassaysPreprint
Lassaunière, R., Frische, A., Harboe, Z. B., Nielsen, A. C. Y., Fomsgaard, A., Krogfelt, K. A., & Jørgensen, C. S. (2020) · medRxiv · doi:10.1101/2020.04.09.20056325 · Cited by 317 · see citing works
MERS-CoV Antibody Responses 1 Year after Symptom Onset, South Korea, 2015
Choe, P. G., Perera, R. A. P. M., Park, W. B., Song, K.-H., Bang, J. H., Kim, E. S., Kim, H. B., Ko, L. W. R., Park, S. W., Kim, N.-J., et al. (2017) · Emerging Infectious Diseases · doi:10.3201/eid2307.170310 · Cited by 227 · see citing works
Serology for SARS-CoV-2: Apprehensions, opportunities, and the path forward
Bryant, J. E., Azman, A. S., Ferrari, M. J., Arnold, B. F., Boni, M. F., Boum, Y., Hayford, K. T., Luquero, F. J., Mina, M. J., Rodríguez-Barraquer, I., et al. (2020) · Science Immunology · doi:10.1126/sciimmunol.abc6347 · Cited by 142 · see citing works
Herd immunity is not a realistic exit strategy during a COVID-19 outbreakPreprint
Slot, E., Hogema, B. M., Reusken, C. B. E. M., Reimerink, J. H. J., Molier, M., Karregat, J. H. M., IJlst, J., Novotny, V. M. J., van Lier, R. A. W., & Zaaijer, H. L. (2020) · Research Square · doi:10.21203/rs.3.rs-25862/v1 · Cited by 55 · see citing works
Flagged first, then by citations
1 of the 10 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Transmission of SARS-CoV-2 on mink farms between humans and mink and back to humans
Munnink, B. B. O., Sikkema, R. S., Nieuwenhuijse, D. F., Molenaar, R. J., Münger, E., Molenkamp, R., van der Spek, A. N., Tolsma, P., Rietveld, A., Brouwer, M., et al. (2020) · Science · doi:10.1126/science.abe5901 · Cited by 1,087 · see citing works
Self-citationAntibody Status and Incidence of SARS-CoV-2 Infection in Health Care Workers
Lumley, S. F., O’Donnell, D., Stoesser, N. E., Matthews, P. C., Howarth, A., Hatch, S. B., Marsden, B. D., Cox, S., James, T., Warren, F., et al. (2021) · New England Journal of Medicine · doi:10.1056/nejmoa2034545 · Cited by 940 · see citing works
Humoral Immune Response to SARS-CoV-2 in Iceland
Gudbjartsson, D. F., Norddahl, G. L., Melsted, P., Gunnarsdottir, K., Holm, H., Eythorsson, E., Arnthorsson, A. O., Helgason, D., Bjarnadottir, K., Ingvarsson, R. F., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2026116 · Cited by 903 · see citing works
Model-informed COVID-19 vaccine prioritization strategies by age and serostatus
Bubar, K. M., Reinholt, K., Kissler, S. M., Lipsitch, M., Cobey, S., Grad, Y. H., & Larremore, D. B. (2021) · Science · doi:10.1126/science.abe6959 · Cited by 775 · see citing works
van Kampen, J. J. A., van de Vijver, D. A. M. C., Fraaij, P. L. A., Haagmans, B. L., Lamers, M. M., Okba, N., van den Akker, J. P. C., Endeman, H., Gommers, D. A. M. P. J., Cornelissen, J. J., et al. (2021) · Nature Communications · doi:10.1038/s41467-020-20568-4 · Cited by 838 · see citing works
Self-citationGeers, D., Shamier, M. C., Bogers, S., den Hartog, G., Gommers, L., Nieuwkoop, N. N., Schmitz, K. S., Rijsbergen, L. C., van Osch, J. A. T., Dijkhuizen, E., et al. (2021) · Science Immunology · doi:10.1126/sciimmunol.abj1750 · Cited by 529 · see citing works
Self-citationCOVID-19: Discovery, diagnostics and drug development
Asselah, T., Durantel, D., Pasmant, É., Lau, G. K. K., & Schinazi, R. F. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2020.09.031 · Cited by 476 · see citing works
Performance characteristics of five immunoassays for SARS-CoV-2: a head-to-head benchmark comparison
Ainsworth, M. A., Andersson, M. I., Auckland, K., Baillie, J. K., Barnes, E. J., Beer, S., Beveridge, A., Bibi, S., Blackwell, L., Borak, M., et al. (2020) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(20)30634-4 · Cited by 337 · see citing works
Gharbharan, A., Jordans, C. C. E., GeurtsvanKessel, C. H., den Hollander, J. G., Karim, F., Mollema, F. P. N., Stalenhoef, J. E., Dofferhoff, A. S. M., Ludwig, I., Koster, A., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23469-2 · Cited by 347 · see citing works
Self-citationA high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
Muruato, A. E., Fontes-Garfias, C. R., Ren, P., Garcia-Blanco, M. A., Menachery, V. D., Xie, X., & Shi, P.-Y. (2020) · Nature Communications · doi:10.1038/s41467-020-17892-0 · Cited by 319 · see citing works
Autoinflammatory and autoimmune conditions at the crossroad of COVID-19
Rodríguez, Y., Novelli, L., Rojas, M., Santis, M. D., Acosta‐Ampudia, Y., Monsalve, D. M., Ramı́rez-Santana, C., Costanzo, A., Ridgway, W. M., Ansari, A. A., et al. (2020) · Journal of Autoimmunity · doi:10.1016/j.jaut.2020.102506 · Cited by 312 · see citing works
Muecksch, F., Wise, H., Batchelor, B., Squires, M., Semple, E., Richardson, C., McGuire, J., Clearly, S., Furrie, E., Greig, N., et al. (2021) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiaa659 · Cited by 308 · see citing works
COVID-19 Antibody Tests and Their Limitations
Liu, G., & Rusling, J. F. (2021) · ACS Sensors · doi:10.1021/acssensors.0c02621 · Cited by 219 · see citing works
Figueiredo‐Campos, P., Blankenhaus, B., Mota, C., Gomes, A., Serrano, M., Ariotti, S., da Costa, C. J. M., Nunes‐Cabaço, H., Mendes, A. M., Gaspar, P., et al. (2020) · European Journal of Immunology · doi:10.1002/eji.202048970 · Cited by 203 · see citing works
COVID-19 vaccines in patients with cancer: immunogenicity, efficacy and safety
Fendler, A., de Vries, E. G. E., GeurtsvanKessel, C. H., Haanen, J. B. A. G., Wormann, B. J., Turajlic, S., & von Lilienfeld‐Toal, M. (2022) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-022-00610-8 · Cited by 205 · see citing works
Self-citationAntibody tests for identification of current and past infection with SARS-CoV-2
Fox, T., Geppert, J., Dinnes, J., Scandrett, K., Bigio, J., Sulis, G., Hettiarachchi, D., Mathangasinghe, Y., Weeratunga, P. N., Wickramasinghe, D., et al. (2022) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd013652.pub2 · Cited by 204 · see citing works
de Alwis, R., Gan, E. S., Chen, S., Leong, Y. S., Tan, H. C., Zhang, S. L., Yau, C., Low, J. G. H., Kalimuddin, S., Matsuda, D., et al. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.04.001 · Cited by 191 · see citing works
Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset
Kalimuddin, S., Tham, C. Y. L., Qui, M., de Alwis, R., Sim, J. X. Y., Lim, J. M. E., Tan, H.-C., Syenina, A., Zhang, S. L.-X., Bert, N. L., et al. (2021) · Med · doi:10.1016/j.medj.2021.04.003 · Cited by 179 · see citing works
Review of Current Advances in Serologic Testing for COVID-19
Freire, A. P. E., Akgun, Y., Mana, A. F. A., Tjendra, Y., Millan, N. C., Gomez-Fernandez, C. R., & Cray, C. (2020) · American Journal of Clinical Pathology · doi:10.1093/ajcp/aqaa112 · Cited by 167 · see citing works
Immunity to SARS‐CoV‐2 induced by infection or vaccination
Dopico, X. C., Ols, S., Loré, K., & Hedestam, G. B. K. (2021) · Journal of Internal Medicine · doi:10.1111/joim.13372 · Cited by 171 · see citing works
den Hartog, G., Schepp, R. M., Kuijer, M., GeurtsvanKessel, C., van Beek, J., Rots, N., Koopmans, M. P. G., van der Klis, F. R. M., & van Binnendijk, R. S. (2020) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiaa479 · Cited by 137 · see citing works
Self-citationValidation of a commercially available SARS-CoV-2 serological immunoassay
Meyer, B., Torriani, G., Yerly, S., Mazza, L., Calame, A., Arm‐Vernez, I., Zimmer, G., Agoritsas, T., Stirnemann, J., Spechbach, H., et al. (2020) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2020.06.024 · Cited by 156 · see citing works
Poletti, P., Tirani, M., Cereda, D., Trentini, F., Guzzetta, G., Sabatino, G., Marziano, V., Castrofino, A., Grosso, F. M., Castillo, G. D., et al. (2021) · JAMA Network Open · doi:10.1001/jamanetworkopen.2021.1085 · Cited by 155 · see citing works
Padoan, A., Bonfante, F., Pagliari, M., Bortolami, A., Negrini, D., Zuin, S., Bozzato, D., Cosma, C., Sciacovelli, L., & Plebani, M. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.103101 · Cited by 145 · see citing works
How to interpret and use COVID-19 serology and immunology tests
Ong, D. S. Y., Fragkou, P. C., Schweitzer, V. A., Chemaly, R. F., Moschopoulos, C. D., & Skevaki, C. L. (2021) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2021.05.001 · Cited by 144 · see citing works
Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories
Harritshøj, L. H., Gybel‐Brask, M., Afzal, S., Kamstrup, P. R., Jørgensen, C. S., Thomsen, M. K., Hilsted, L. M., Friis-Hansen, L. J., Szecsi, P. B., Pedersen, L., et al. (2021) · Journal of Clinical Microbiology · doi:10.1128/jcm.02596-20 · Cited by 134 · see citing works
Laboratory testing for the diagnosis of COVID-19
Lai, C. K. C., & Lam, W. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.10.069 · Cited by 137 · see citing works
Eyre, D. W., Lumley, S. F., Wei, J., Cox, S., James, T., Justice, A., Jesuthasan, G., O'Donnell, D., Howarth, A., Hatch, S. B., et al. (2021) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2021.05.041 · Cited by 131 · see citing works
Validation and clinical evaluation of a SARS-CoV-2 surrogate virus neutralisation test (sVNT)
Meyer, B., Reimerink, J. H. J., Torriani, G., Brouwer, F., Godeke, G.-J., Yerly, S., Hoogerwerf, M. N., Vuilleumier, N., Kaiser, L., Eckerle, I., et al. (2020) · Emerging Microbes & Infections · doi:10.1080/22221751.2020.1835448 · Cited by 130 · see citing works
Annasamy, G., Kumar, N. A., & Anand, S. (2021) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2021.116205 · Cited by 131 · see citing works
Rasmi, Y., Li, X., Khan, J., Özer, T., & Choi, J. R. (2021) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-021-03377-6 · Cited by 130 · see citing works
Immunogenicity and Reactogenicity of Vaccine Boosters after Ad26.COV2.S Priming
Sablerolles, R. S. G., Rietdijk, W. J. R., Goorhuis, A., Postma, D. F., Visser, L. G., Geers, D., Schmitz, K. S., Garrido, H. M. G., Koopmans, M., Dalm, V. A. S. H., et al. (2022) · New England Journal of Medicine · doi:10.1056/nejmoa2116747 · Cited by 123 · see citing works
Self-citationA comparative review of immunoassays for COVID-19 detection
Mohit, E., Rostami, Z., & Vahidi, H. (2021) · Expert Review of Clinical Immunology · doi:10.1080/1744666x.2021.1908886 · Cited by 116 · see citing works
Association between SARS-CoV-2 Neutralizing Antibodies and Commercial Serological Assays
Tang, M. S., Case, J. B., Franks, C. E., Chen, R. E., Anderson, N. W., Henderson, J. P., Diamond, M., Gronowski, A. M., & Farnsworth, C. W. (2020) · Clinical Chemistry · doi:10.1093/clinchem/hvaa211 · Cited by 113 · see citing works
Wynberg, E., van Willigen, H. D. G., Dijkstra, M., Boyd, A. C., Kootstra, N. A., van den Aardweg, J. G., van Gils, M. J., Matser, A. A., de Wit, M. R., Leenstra, T., et al. (2021) · Clinical Infectious Diseases · doi:10.1093/cid/ciab759 · Cited by 105 · see citing works
Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
Gong, F., Wei, H.-X., Li, Q., Liu, L., & Li, B. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.682405 · Cited by 103 · see citing works
Tran, V. V., Tran, N. H. T., Hwang, H. S., & Chang, M. (2021) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2021.113192 · Cited by 100 · see citing works
Zhang, Z., Bi, Q., Fang, S., Wei, L., Wang, X., He, J., Wu, Y., Liu, X., Gao, W., Zhang, R., et al. (2021) · The Lancet Microbe · doi:10.1016/s2666-5247(20)30200-7 · Cited by 91 · see citing works
De Novo Discovery of High-Affinity Peptide Binders for the SARS-CoV‑2 Spike Protein
Pomplun, S. J., Jbara, M., Quartararo, A. J., Zhang, G., Brown, J. S., Lee, Y., Ye, X., Hanna, S., & Pentelute, B. L. (2020) · ACS Central Science · doi:10.1021/acscentsci.0c01309 · Cited by 89 · see citing works
Tang, Y., Song, T., Gao, L., Yin, S., Ma, M., Tan, Y., Wu, L., Yang, Y., Wang, Y., Lin, T., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32371-4 · Cited by 84 · see citing works
Simón, D., Taşçılar, K., Krönke, G., Kleyer, A., Zaiss, M. M., Heppt, F., Meder, C. H., Atreya, R. N., Klenske, E., Dietrich, P., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-17703-6 · Cited by 83 · see citing works
Fenwick, C. W., Turelli, P., Pellaton, C., Farina, A., Campos, J., Raclot, C., Pojer, F., Cagno, V., Gonseth, S., D’Acremont, V., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abi8452 · Cited by 86 · see citing works
Kumar, R., Yeni, C. M., Utami, N. A., Masand, R., Asrani, R. K., Patel, S. K., Kumar, A., Yatoo, M. I., Tiwari, R., Natesan, S., et al. (2021) · Journal of Infection and Public Health · doi:10.1016/j.jiph.2021.04.005 · Cited by 82 · see citing works
Vogelzang, E. H., Loeff, F. C., Derksen, N. I. L., Kruithof, S., Heer, P. O., van Mierlo, G. J., Linty, F., Mok, J. Y., van Esch, W. J. E., de Bruin, S., et al. (2020) · The Journal of Immunology · doi:10.4049/jimmunol.2000767 · Cited by 76 · see citing works
Machado, B. A. S., Hodel, K. V. S., Júnior, V. G. B., Soares, M. B. P., & Badaró, R. J. D. S. (2020) · Viruses · doi:10.3390/v13010040 · Cited by 77 · see citing works
Mekonnen, D. S., Mengist, H. M., Derbie, A., Nibret, E., Munshea, A., He, H., Li, B., & Jin, T. (2020) · Reviews in Medical Virology · doi:10.1002/rmv.2181 · Cited by 80 · see citing works
Humoral immune mechanisms involved in protective and pathological immunity during COVID-19
Widjaja, G., Jalil, A. T., Rahman, H. S., Abdelbasset, W. K., Bokov, D. O., Suksatan, W., Ghaebi, M., Marofi, F., Navashenaq, J. G., Jadidi‐Niaragh, F., et al. (2021) · Human Immunology · doi:10.1016/j.humimm.2021.06.011 · Cited by 75 · see citing works
Bal, A., Pozzetto, B., Trabaud, M., Escuret, V., Rabilloud, M., Langlois, C., Paul, A., Guibert, N., d’Aubarede, C., Massardier-Pilonchery, A., et al. (2021) · Clinical Chemistry · doi:10.1093/clinchem/hvaa336 · Cited by 74 · see citing works
SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity
Nielsen, S. S. F., Vibholm, L. K., Monrad, I., Olesen, R., Frattari, G. S., Pahus, M. H., Højen, J. F., Gunst, J. D., Erikstrup, C., Holleufer, A., et al. (2021) · EBioMedicine · doi:10.1016/j.ebiom.2021.103410 · Cited by 75 · see citing works
Guillain-Barré syndrome after SARS-CoV-2 infection in an international prospective cohort study
Luijten, L. W. G., Leonhard, S. E., van der Eijk, A. A., Doets, A. Y., Appeltshauser, L., Arends, S., Attarian, S., Benedetti, L., Briani, C., Casasnovas, C., et al. (2021) · Brain · doi:10.1093/brain/awab279 · Cited by 73 · see citing works
Emmerich, P., Murawski, C., Ehmen, C., von Possel, R., Pekarek, N., Oestereich, L., Duraffour, S., Pahlmann, M., Struck, N. S., Eibach, D., et al. (2021) · Tropical Medicine & International Health · doi:10.1111/tmi.13569 · Cited by 72 · see citing works
Augustine, R., Das, S., Hasan, A., Abhilash, S., Salam, S. A., Augustine, P., Dalvi, Y. B., Varghese, R., Primavera, R., Yassine, H. M., et al. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9103372 · Cited by 71 · see citing works
Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections
Wang, P. (2020) · Journal of Virological Methods · doi:10.1016/j.jviromet.2020.113919 · Cited by 70 · see citing works
Zhao, S., Schuurman, N., Li, W., Wang, C., Smit, L. A. M., Broens, E. M., Wagenaar, J. A., van Kuppeveld, F. J. M., Bosch, B., & Egberink, H. F. (2021) · Emerging infectious diseases · doi:10.3201/eid2705.204055 · Cited by 69 · see citing works
Poletti, P., Tirani, M., Cereda, D., Guzzetta, G., Trentini, F., Marziano, V., Toso, C., Piatti, A., Piccarreta, R., Melegaro, A., et al. (2021) · JAMA Network Open · doi:10.1001/jamanetworkopen.2021.15699 · Cited by 69 · see citing works
Zhou, Y., Chen, Y., Liu, W., Fang, H., Li, X., Hou, L., Liu, Y., Lai, W., Huang, X., & Xiong, Y. (2021) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2021.130139 · Cited by 68 · see citing works
Immunogenicity of the mRNA-1273 COVID-19 vaccine in adult patients with inborn errors of immunity
van Leeuwen, L. P. M., GeurtsvanKessel, C. H., Ellerbroek, P. M., de Bree, G. J., Potjewijd, J., Rutgers, A., Jolink, H., van de Veerdonk, F. L., Gorp, E. C. M. V., de Wilt, F., et al. (2022) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2022.04.002 · Cited by 67 · see citing works
Self-citationGong, F., Wei, H.-X., Qi, J., Ma, H., Liu, L., Weng, J., Zheng, X., Li, Q., Zhao, D., Fang, H., et al. (2021) · ACS Sensors · doi:10.1021/acssensors.1c00773 · Cited by 68 · see citing works
Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry
Dzimianski, J. V., Lorig‐Roach, N., O’Rourke, S. M., Alexander, D. L., Kimmey, J. M., & DuBois, R. M. (2020) · Scientific Reports · doi:10.1038/s41598-020-78895-x · Cited by 66 · see citing works
The Prevalence of Antibodies to SARS-CoV-2 Among Blood Donors in China
Chang, L., Hou, W., Zhao, L., Zhang, Y., Wang, Y., Wu, L., Xu, T., Wang, L., Wang, J., Ma, J., et al. (2020) · Nature Communications · doi:10.1038/s41467-021-21503-x · Cited by 67 · see citing works
Zhang, J., Lin, H., Ye, B., Zhao, M. J., Zhan, J., Dong, S., Guo, Y., Zhao, Y., Li, M., Liu, S., et al. (2021) · Clinical Infectious Diseases · doi:10.1093/cid/ciab884 · Cited by 66 · see citing works
Calvo-Lozano, O., Sierra, M., Soler, M., Estévez, M. ‐., Chiscano‐Camón, L., Ruiz-Sanmartín, A., Ruiz‐Rodríguez, J. C., Ferrer, R., González‐López, J. J., Esperalba, J., et al. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c03850 · Cited by 66 · see citing works
Maor, ., Cohen, D., Paran, N., Israely, T., Ezra, V., Axelrod, O., Shinar, E., Izak, M., Rahav, G., Rahimi‐Levene, N., et al. (2020) · EClinicalMedicine · doi:10.1016/j.eclinm.2020.100525 · Cited by 65 · see citing works
Wildner, N. H., Ahmadi, P., Schulte, S., Brauneck, F., Kohsar, M., Lütgehetmann, M., Beisel, C., Addo, M. M., Haag, F., & Wiesch, J. S. Z. (2020) · Journal of Leukocyte Biology · doi:10.1002/jlb.5cova0620-370rr · Cited by 64 · see citing works
A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
Yao, Z., Drecun, L., Aboualizadeh, F., Kim, S. J., Li, Z., Wood, H., Valcourt, E. J., Manguiat, K., Plenderleith, S. W., Yip, L., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22102-6 · Cited by 65 · see citing works
SARS-CoV-2 Serologic Assay Needs for the Next Phase of the US COVID-19 Pandemic Response
Gundlapalli, A. V., Salerno, R. M., Brooks, J. T., Averhoff, F. M., Petersen, L. R., McDonald, L. C., Iademarco, M. F., Response, C. C.-1., Carroll, Y. I., Gorwitz, R. J., et al. (2020) · Open Forum Infectious Diseases · doi:10.1093/ofid/ofaa555 · Cited by 62 · see citing works
Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting
Müller, L., Ostermann, P. N., Walker, A., Wienemann, T., Mertens, A., Adams, O., Andrée, M., Hauka, S., Lübke, N., Keitel, V., et al. (2021) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-021-04169-7 · Cited by 59 · see citing works
Mandolo, J., Msefula, J., Henrion, M. Y. R., Brown, C., Moyo, B., Samon, A., Moyo-Gwete, T., Makhado, Z., Ayres, F., Motlou, T., et al. (2021) · BMC Medicine · doi:10.1186/s12916-021-02187-y · Cited by 61 · see citing works
Verdun, C. M., Fuchs, T., Harár, P., Elbrächter, D., Fischer, D. S., Berner, J., Grohs, P., Theis, F. J., & Krahmer, F. (2021) · Frontiers in Public Health · doi:10.3389/fpubh.2021.583377 · Cited by 61 · see citing works
Commercialized diagnostic technologies to combat SARS-CoV2: Advantages and disadvantages
Pérez‐López, B., & Mir, M. (2020) · Talanta · doi:10.1016/j.talanta.2020.121898 · Cited by 60 · see citing works
Probability of symptoms and critical disease after SARS-CoV-2 infectionPreprint
Poletti, P., Tirani, M., Cereda, D., Trentini, F., Guzzetta, G., Sabatino, G., Marziano, V., Castrofino, A., Grosso, F., Del Castillo, G., et al. (2020) · arXiv (Cornell University) · doi:10.48550/arxiv.2006.08471 · Cited by 60 · see citing works
Qu, J.-H., Leirs, K., Maes, W., Imbrechts, M., Callewaert, N., Lagrou, K., Geukens, N., Lammertyn, J., & Spasić, D. (2022) · ACS Sensors · doi:10.1021/acssensors.1c02215 · Cited by 59 · see citing works
Humoral Immune Response to SARS-CoV-2
Herroelen, P. H., Martens, G. A., Smet, D. D., Swaerts, K., & Decavele, A.-S. C. (2020) · American Journal of Clinical Pathology · doi:10.1093/ajcp/aqaa140 · Cited by 57 · see citing works
Trabaud, M., Icard, V., Milon, M.-P., Bal, A., Lina, B., & Escuret, V. (2020) · Journal of Clinical Virology · doi:10.1016/j.jcv.2020.104613 · Cited by 58 · see citing works
High seroprevalence but short‐lived immune response to SARS‐CoV‐2 infection in Paris
Anna, F., Goyard, S., Lalanne, A. I., Névo, F., Gransagne, M., Souque, P., Louis, D., Gillon, V., Turbiez, I., Bidard, F., et al. (2020) · European Journal of Immunology · doi:10.1002/eji.202049058 · Cited by 58 · see citing works
Bright, B., Babalola, C. P., Sam-Agudu, N. A., Onyeaghala, A. A., Olatunji, A., Aduh, U., Sobande, P. O., Crowell, T. A., Tebeje, Y. K., Phillip, S., et al. (2021) · Globalization and Health · doi:10.1186/s12992-021-00668-6 · Cited by 59 · see citing works
Ngere, I., Dawa, J., Hunsperger, E., Otieno, N. A., Masika, M., Amoth, P., Makayotto, L., Nasimiyu, C., Gunn, B. M., Nyawanda, B. O., et al. (2021) · International Journal of Infectious Diseases · doi:10.1016/j.ijid.2021.08.062 · Cited by 57 · see citing works
Recent Progress in Nanotechnology for COVID-19 Prevention, Diagnostics and Treatment
Rasmi, Y., Sahbani, S. K., Nemati, M., & Choi, J. R. (2021) · Nanomaterials · doi:10.3390/nano11071788 · Cited by 58 · see citing works
Li, Y., Lai, D., Lei, Q., Xu, Z., Wang, F., Hou, H., Chen, L., Wu, J., Ren, Y., Ma, M., et al. (2021) · Cellular and Molecular Immunology · doi:10.1038/s41423-020-00612-5 · Cited by 55 · see citing works
Padoan, A., Bonfante, F., Cosma, C., Chiara, C. D., Sciacovelli, L., Pagliari, M., Bortolami, A., Costenaro, P., Musso, G., Basso, D., et al. (2021) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2021-0313 · Cited by 57 · see citing works
Hellemons, M. E., Huijts, S., Bek, L. M., Berentschot, J. C., Nakshbandi, G., Schurink, C. A. M., Vlake, J. H., van Genderen, M. E., van Bommel, J., Gommers, D., et al. (2021) · Annals of the American Thoracic Society · doi:10.1513/annalsats.202103-340oc · Cited by 55 · see citing works
Liu, K., Han, Y.-J., Wu, G.-H., Huang, K.-Y. A., & Huang, P.-N. (2022) · Viruses · doi:10.3390/v14071560 · Cited by 54 · see citing works
Seroprevalence of SARS-CoV-2 in Niger State: Pilot Cross-Sectional Study
Majiya, H., Aliyu‐Paiko, M., Balogu, T. V., Musa, D. A., Salihu, I. M., Kawu, A. A., Bashir, I. Y., Sani, A. R., John, B., Muhammad, A. T., et al. (2023) · JMIRx Med · doi:10.2196/29587 · Cited by 53 · see citing works
Berkenbrock, J. A., Grecco-Machado, R., & Achenbach, S. C. (2020) · Proceedings of the Royal Society A Mathematical Physical and Engineering Sciences · doi:10.1098/rspa.2020.0398 · Cited by 53 · see citing works
Interferon-α2 Auto-antibodies in Convalescent Plasma Therapy for COVID-19
Raadsen, M. P., Gharbharan, A., Jordans, C. C. E., Mykytyn, A. Z., Lamers, M. M., van den Doel, P. B., Endeman, H., van den Akker, J. P. C., GeurtsvanKessel, C. H., Koopmans, M., et al. (2021) · Journal of Clinical Immunology · doi:10.1007/s10875-021-01168-3 · Cited by 52 · see citing works
Self-citationUnique Severe COVID-19 Placental Signature Independent of Severity of Clinical Maternal Symptoms
Husen, M., van der Meeren, L. E., Verdijk, R. M., Fraaij, P. L. A., van der Eijk, A. A., Koopmans, M., Freeman, L. M., Bogers, H., Trietsch, M. D., Reiss, I. K. M., et al. (2021) · Viruses · doi:10.3390/v13081670 · Cited by 51 · see citing works
Self-citationCotugno, N., Ruggiero, A., Bonfante, F., Petrara, M. R., Zicari, S., Pascucci, G. R., Zangari, P., Ioris, M. A. D., Santilli, V., Manno, E. C., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.108852 · Cited by 50 · see citing works
Muecksch, F., Wise, H., Templeton, K., Batchelor, B., Squires, M., McCance, K., Jarvis, L., Malloy, K., Furrie, E., Richardson, C., et al. (2022) · The Lancet Microbe · doi:10.1016/s2666-5247(22)00090-8 · Cited by 34 · see citing works
Goh, Y. S., Chavatte, J., Jieling, A. L., Lee, B. T. K., Hor, P. X., Amrun, S. N., Lee, C. Y., Chee, R. S., Wang, B., Lee, C. Y., et al. (2020) · Cell Reports Medicine · doi:10.1016/j.xcrm.2021.100193 · Cited by 47 · see citing works
Zhu, W., Hutchison, J. A., Dong, M., & Li, M. (2021) · ACS Sensors · doi:10.1021/acssensors.1c00393 · Cited by 48 · see citing works
Germanio, C. D., Simmons, G., Kelly, K. K., Martinelli, R., Darst, O., Azimpouran, M., Stone, M., Hazegh, K. E., Grebe, E., Zhang, S., et al. (2021) · Transfusion · doi:10.1111/trf.16555 · Cited by 44 · see citing works
Diagnostic performance of commercially available COVID-19 serology tests in Brazil
Cota, G., Freire, M. L., de Souza, C. S., Pedras, M. J., Saliba, J. W., de Faria, V. C. S., Alves, L. L., Rabello, A., & de Avelar, D. M. (2020) · International Journal of Infectious Diseases · doi:10.1016/j.ijid.2020.10.008 · Cited by 46 · see citing works
Coyer, L., Boyd, A. C., Schinkel, J., Agyemang, C. O., Galenkamp, H., Koopman, A. D. M., Leenstra, T., van Duijnhoven, Y. T. H. P., van Charante, E. P. M., van den Born, B. H., et al. (2021) · The Lancet Regional Health - Europe · doi:10.1016/j.lanepe.2021.100284 · Cited by 46 · see citing works
Ali, M. A., Hu, C., Zhang, F., Jahan, S., Yuan, B., Saleh, M. S., Gao, S., & Panat, R. P. (2022) · Journal of Medical Virology · doi:10.1002/jmv.27591 · Cited by 46 · see citing works
Peng, R., Pan, Y., Li, Z., Qin, Z., Rini, J. M., & Liu, X. (2021) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2021.113762 · Cited by 44 · see citing works
Comparison of different serological assays for SARS-CoV-2 in real life
Brochot, É., Demey, B., Handala, L., François, C., Duverlie, G., & Castelain, S. (2020) · Journal of Clinical Virology · doi:10.1016/j.jcv.2020.104569 · Cited by 43 · see citing works
Xu, L., Ramadan, S., Rosa, B. M. G., Zhang, Y., Yin, T., Torres, E., Shaforost, O., Panagiotopoulos, A., Li, B., Kerherve, G., et al. (2022) · Sensors & Diagnostics · doi:10.1039/d2sd00076h · Cited by 43 · see citing works
Semmler, G., Traugott, M. T., Graninger, M., Hoepler, W., Seitz, T., Kelani, H., Karolyi, M., Pawelka, E., Cruz, S. A. D. L., Puchhammer‐Stöckl, E., et al. (2021) · Journal of Clinical Microbiology · doi:10.1128/jcm.02890-20 · Cited by 41 · see citing works
Valdivia, A., Torres, I., Latorre, V., Francés‐Gómez, C., Albert, E., Gozalbo‐Rovira, R., Alcaráz, M. J., Buesa, J., Rodríguez‐Díaz, J., Geller, R., et al. (2021) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-020-04128-8 · Cited by 40 · see citing works
Evaluation of a multi-species SARS-CoV-2 surrogate virus neutralization test
Embregts, C. W. E., Verstrepen, B. E., Langermans, J. A. M., Böszörményi, K. P., Sikkema, R. S., de Vries, R. D., Hoffmann, D., Wernike, K., Smit, L. A. M., Zhao, S., et al. (2021) · One Health · doi:10.1016/j.onehlt.2021.100313 · Cited by 35 · see citing works
Self-citation
- Authors
- GeurtsvanKessel, C. H., Okba, N. M. A., Igloi, Z., Bogers, S., Embregts, C. W. E., Laksono, B. M., Leijten, L., Rokx, C., Rijnders, B., Rahamat-Langendoen, J., van den Akker, J. P. C., van Kampen, J. J. A., van der Eijk, A. A., van Binnendijk, R. S., Haagmans, B., & Koopmans, M.
- Affiliations
- Erasmus MC (NL)
- Radboud University Medical Center (NL)
- Corresponding author
- GeurtsvanKessel, Corine H.
- ORCID
- Venue
- Nature Communications
- Published
- 2020-07-06
- Volume
- 11
- Issue
- 1
- Pages
- 3436-3436
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC7338506
- OpenAlex
- W3039328844
- Cited by
- 361
- References
- 10
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 15 · 2023: 33 · 2022: 66 · 2021: 165 · 2020: 68
- FWCI
- 10.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- medRxiv (acceptedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Radboud Repository (Radboud University) (submittedVersion)
- Rivm Repository (Netherlands National Institute for Public Health and the Environment) (submittedVersion)
- Rivm (National Institute for Public Health and the Environment) (submittedVersion)
- Rivm (National Institute for Public Health and the Environment) (submittedVersion, other-oa)
- Radboud Repository (Radboud University) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Health Sciences › Medicine › Infectious Diseases › COVID-19 Clinical Research Studies
- Keywords
- COVID-19 pandemic, SARS-CoV-2, serological assays, antibody detection, ELISA, receptor binding domain, virus-neutralizing antibodies, assay validation, diagnostic accuracy, exposure assessment, mass screening, disease severity
- MeSH terms
- Betacoronavirus, COVID-19, SARS-CoV-2, COVID-19 Testing, Antibodies, Viral, Enzyme-Linked Immunosorbent Assay, Humans, Luminescent Measurements, Neutralization Tests, Pneumonia, Viral, Sensitivity and Specificity, Serologic Tests, Coronavirus Infections, Clinical Laboratory Techniques, Antibodies, Neutralizing, High-Throughput Screening Assays, Pandemics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
