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Cited by 34Cellular Mechanics and InteractionsCellular transport and secretionLipid Membrane Structure and Behavior

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Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles

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Sitarska, Ewa, Almeida, Silvia Dias, Beckwith, Marianne Sandvold, Stopp, Julian, Czuchnowski, Jakub, Siggel, Marc, Roessner, Rita, Tschanz, Aline, Ejsing, Christer, Schwab, Yannick, Kosinski, Jan, Sixt, Michael, Kreshuk, Anna, Erzberger, Anna, Diz-Muñoz, Alba·2023·Nature Communications
DOI 10.1038/s41467-023-41173-1PMC PMC10499897

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Abstract

To navigate through diverse tissues, migrating cells must balance persistent self-propelled motion with adaptive behaviors to circumvent obstacles. We identify a curvature-sensing mechanism underlying obstacle evasion in immune-like cells. Specifically, we propose that actin polymerization at the advancing edge of migrating cells is inhibited by the curvature-sensitive BAR domain protein Snx33 in regions with inward plasma membrane curvature. The genetic perturbation of this machinery reduces the cells’ capacity to evade obstructions combined with faster and more persistent cell migration in obstacle-free environments. Our results show how cells can read out their surface topography and utilize actin and plasma membrane biophysics to interpret their environment, allowing them to adaptively decide if they should move ahead or turn away. On the basis of our findings, we propose that the natural diversity of BAR domain proteins may allow cells to tune their curvature sensing machinery to match the shape characteristics in their environment.

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Metadata

Authors
Sitarska, E., Almeida, S. D., Beckwith, M. S., Stopp, J., Czuchnowski, J., Siggel, M., Roessner, R., Tschanz, A., Ejsing, C., Schwab, Y., Kosinski, J., Sixt, M., Kreshuk, A., Erzberger, A., & Diz-Muñoz, A.
Affiliations
  • Heidelberg University (DE)
  • European Molecular Biology Laboratory (DE)
  • Institute of Science and Technology Austria (AT)
  • University of Southern Denmark (DK)
Corresponding author
Diz-Muñoz, Alba
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2023-09-13
Volume
14
Issue
1
Pages
5644-5644
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-023-41173-1
PMC
PMC10499897
OpenAlex
W3149773180
Cited by
34
References
4
Citations by year
2026: 10 · 2025: 14 · 2024: 6 · 2023: 2
FWCI
4.63
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • University of Southern Denmark Research Portal (University of Southern Denmark) (publishedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • Desy Publications Database (Deutsches Elektronen-Synchrotron DESY) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron)
Funders
  • European Molecular Biology Laboratory
  • Joachim Herz Stiftung
  • the BEST program funding by FCT
  • the EMBL Interdisciplinary Postdocs (EIPOD) programme under Marie Sklodowska-Curie COFUND actions MSCA-COFUND-FP
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Keywords
cell migration, curvature sensing, BAR domain proteins, actin polymerization, cell polarity, cell shape
MeSH terms
Actins, Adaptation, Psychological, Biophysics, Cell Membrane, Cell Movement

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