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Cited by 7Cellular transport and secretionProtein Kinase Regulation and GTPase SignalingReceptor Mechanisms and Signaling

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Structural basis for Rab6 activation by the Ric1-Rgp1 complex

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Feathers, J. Ryan, Vignogna, Ryan C., Fromme, J. Christopher·2024·Nature Communications
DOI 10.1038/s41467-024-54869-9PMC PMC11618376

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Abstract

Rab GTPases act as molecular switches to regulate organelle homeostasis and membrane trafficking. Rab6 plays a central role in regulating cargo flux through the Golgi and is activated via nucleotide exchange by the Ric1-Rgp1 protein complex. Ric1-Rgp1 is conserved throughout eukaryotes but the structural and mechanistic basis for its function has not been established. Here we report the cryoEM structure of a Ric1-Rgp1-Rab6 complex representing a key intermediate of the nucleotide exchange reaction. This structure reveals the overall architecture of the complex and enabled us to identify interactions critical for proper recognition and activation of Rab6 on the Golgi membrane surface. Ric1-Rgp1 interacts with the nucleotide-binding domain of Rab6 using an uncharacterized helical domain, which we establish as a novel RabGEF domain by identifying residues required for Rab6 nucleotide exchange. Unexpectedly, the complex uses an arrestin fold to interact with the Rab6 hypervariable domain, indicating that interactions with the unstructured C-terminal regions of Rab GTPases may be a common specificity mechanism used by their activators. Collectively, our findings provide a detailed mechanistic understanding of regulated Rab6 activation at the Golgi.

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Metadata

Authors
Feathers, J. R., Vignogna, R. C., & Fromme, J. C.
Affiliations
  • Princeton University (US)
  • Cornell University (US)
Corresponding author
Fromme, J. Christopher
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-12-04
Volume
15
Issue
1
Pages
10561-10561
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-024-54869-9
PMC
PMC11618376
OpenAlex
W4404998466
Cited by
7
References
2
Citations by year
2026: 3 · 2025: 4
FWCI
1.57
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35GM136258, F32GM155980)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
Rab6, nucleotide exchange, Golgi apparatus, membrane trafficking, protein complex structure

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