Student or educator? Sign up with your school email and get 6 months of Pro free.
Cited by 6

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa

Ben-David, Yossi, Sporny, Michael, Brochin, Yigal, Piscon, Bar, Roth, Shira, Zander, Itzhak, Nisani, Michal, Shoshani, Sivan, Yaron, Orly, Karako-Lampert, Sarit, Lebenthal-Loinger, Ilana, Danielli, Amos, Opatowsky, Yarden, Banin, Ehud·2025·npj Biofilms and Microbiomes

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41522-025-00710-0

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 55

  1. Hsueh, P. R. et al. Persistence of a multidrug-resistant Pseudomonas aeruginosa clone in an intensive care burn unit. J. Clin. Microbiol 36, 1347–1351 (1998). · doi:10.1128/jcm.36.5.1347-1351.1998
  2. Aloush, V., Navon-venezia, S., Seigman-igra, Y., Cabili, S. & Carmeli, Y. Multidrug-resistant Pseudomonas aeruginosa: risk factors and clinical impact. Society 50, 43–48 (2006).
  3. Kwon, S., Schweizer, M. L. & Perencevich, E. N. National Institute of Allergy and Infectious Disease (NIAID) funding for studies of hospital-associated bacterial pathogens: are funds proportionate to burden of disease?. Antimicrob. Resist Infect. Control 1, 5 (2012). · doi:10.1186/2047-2994-1-5
  4. Hirsch, E. B. & Tam, V. H. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev. Pharmacoecon Outcomes Res. 10, 1–18 (2011).
  5. Hill, D. et al. Antibiotic susceptibility of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions. J. Clin. Microbiol 43, 5085–5090 (2005). · DOI · Paper page
  6. Bjarnsholt, T. The role of bacterial biofilms in chronic infections. APMIS Suppl. https://doi.org/10.1111/apm.12099. (2013). · DOI · Paper page
  7. Moreau-Marquis, S., Stanton, B. & O’Toole, G. Pseudomonas aeruginosa biofilm formation in the cystic fibrosis airway. Pulm. Pharmacol. Ther. 21, 595–599 (2008). · DOI · Paper page
  8. Wozniak, D. J. & Mann, E. E. Pseudomonas biofilm matrix composition and niche biology. FEMS. Microbiol. Rev. 33, 395–401 (2015).
  9. Mikkelsen, H., Sivaneson, M. & Filloux, A. Key two-component regulatory systems that control biofilm formation in Pseudomonas aeruginosa. Environ. Microbiol. 13, 1666–1681 (2011). · DOI · Paper page
  10. Schurr, M. J. Which bacterial biofilm exopolysaccharide is preferred, psl or alginate?. J. Bacteriol. 195, 1623–1626 (2013). · doi:10.1128/jb.00173-13
  11. Valentini, M. & Filloux, A. Biofilms and cyclic di-GMP (c-di-GMP) signaling: lessons from Pseudomonas aeruginosa and other bacteria. J. Biol. Chem. 291, 12547–12555 (2016). · DOI · Paper page
  12. Merritt, J. udithH. et al. Specific control of Pseudomonas aeruginosa surface-associated behaviors by two c-di-GMP diguanylate cyclases. mBio 1, 1–9 (2010). · DOI · Paper page
  13. Petrova, O. E., Cherny, K. E. & Sauer, K. The Pseudomonas aeruginosa diguanylate cyclase GcbA, a homolog of P. Fluorescens GcbA, promotes initial attachment to surfaces, but not biofilm formation, via regulation of motility. J. Bacteriol. 196, 2827–2841 (2014). · DOI
  14. Ha, D.-G. & O’Toole, G. A. c-di-GMP and its effects on biofilm formation and dispersion: a Pseudomonas aeruginosa review. Microbiol. Spectr. 3, MB-0003-2014 (2015). · DOI · Paper page
  15. Park, S. & Sauer, K. Controlling Biofilm Development Through Cyclic di-GMP Signaling. in Advances in Experimental Medicine and Biology Vol. 1386, 69–94 (Springer, 2022). · DOI
  16. Hickman, J. W., Tifrea, D. F. & Harwood, C. S. A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels. Proc. Natl Acad. Sci. USA 102, 14422–14427 (2005). · DOI · Paper page
  17. Borlee, B. R. et al. Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix. Mol. Microbiol. 75, 827–842 (2010). · DOI · Paper page
  18. Moscoso, J. A. et al. The diguanylate cyclase SadC is a central player in Gac/Rsm-mediated biofilm formation in Pseudomonas aeruginosa. J. Bacteriol. 196, 4081–4088 (2014). · DOI
  19. Caiazza, N. ickyC. & O’Toole, G. eorgeA. SadB is required for the transition from reversible to irreversible attachment during biofilm formation by Pseudomonas aeruginosa PA14. J. Bacteriol. 186, 4476–4485 (2004). · DOI · Paper page
  20. Caiazza, N. C., Merritt, J. H., Brothers, K. M. & O’Toole, G. A. Inverse regulation of biofilm formation and swarming motility by Pseudomonas aeruginosa PA14. J. Bacteriol. 189, 3603–3612 (2007). · DOI · Paper page
  21. Merritt, J. H., Brothers, K. M., Kuchma, S. L. & O’Toole, G. A. SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function. J. Bacteriol. 189, 8154–8164 (2007). · DOI · Paper page
  22. Kuchma, S. L. et al. BifA, a cyclic-di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14. J. Bacteriol. 189, 8165–8178 (2007). · DOI · Paper page
  23. Baynham, P. J., Ramsey, D. M., Gvozdyev, B. V., Cordonnier, E. M. & Wozniak, D. J. The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV Pili. J. Bacteriol. 188, 132 (2006). · doi:10.1128/jb.188.1.132-140.2006
  24. Paris, Z., Fleming, I. M. C. & Alfonzo, J. D. Determinants of tRNA editing and modification: avoiding conundrums, affecting function. Semin. Cell Dev. Biol. 23, 269–274 (2012). · DOI
  25. García-Contreras, R., Zhang, X. S., Kim, Y. & Wood, T. K. Protein translation and cell death: The role of rare tRNAs in biofilm formation and in activating dormant phage killer genes. PLoS ONE 3, e2394 (2008). · DOI
  26. Muriel, C. et al. AmrZ is a major determinant of c-di-GMP levels in Pseudomonas fluorescens F113. Sci. Rep. 8, 1–10 (2018). · doi:10.1038/s41598-018-20419-9
  27. Pryor, E. E. et al. The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes. PLoS Pathog. 8, e1002648 (2012). · DOI
  28. Jones, C. J. et al. ChIP-seq and RNA-seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa. PLoS Pathog. 10, e1003984 (2014). · DOI · Paper page
  29. Oladosu, V. I., Park, S. & Sauer, K. Flip the switch: the role of FleQ in modulating the transition between the free-living and sessile mode of growth in Pseudomonas aeruginosa. J. Bacteriol. 206, e0036523 (2024). · DOI
  30. Hou, L., Debru, A., Chen, Q., Bao, Q. & Li, K. AmrZ regulates swarming motility through cyclic di-GMP-dependent motility inhibition and controlling Pel polysaccharide production in Pseudomonas aeruginosa PA14. Front Microbiol. 10, 1847 (2019). · DOI
  31. Jones, C. J., Ryder, C. R., Mann, E. E. & Wozniak, D. J. AmrZ modulates pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon. J. Bacteriol. 195, 1637–1644 (2013). · DOI
  32. Feng, Q. et al. Regulation of Exopolysaccharide Production by ProE, a Cyclic-Di-GMP Phosphodiesterase in Pseudomonas aeruginosa PAO1. Front Microbiol. 11, 1–18 (2020). · DOI
  33. Roth, S. et al. Identification of protein-protein interactions using a magnetic modulation biosensing system. Sens Actuators B Chem. 303, 127228 (2020). · DOI
  34. Zhang, H. et al. Crystal structure of YbaK protein from Haemophilus influenzae (HI1434) at 1.8 A resolution: functional implications. Proteins: Struct., Funct., Genet. 40, 86–97 (2000). · doi:10.1002/(sici)1097-0134(20000701)40:1<86::aid-prot100>3.0.co;2-y
  35. Ghosh, S. & Deutscher, M. P. Oligoribonuclease is an essential component of the mRNA decay pathway. Proc. Natl Acad. Sci. USA 96, 4372–4377 (1999). · DOI · Paper page
  36. Martínez-Granero, F. et al. The Gac-Rsm and SadB signal transduction pathways converge on AlgU to downregulate motility in Pseudomonas fluorescens. PLoS ONE 7, e31765 (2012). · DOI
  37. Lapouge, K., Schubert, M., Allain, F. H. T. & Haas, D. Gac/Rsm signal transduction pathway of γ-proteobacteria: From RNA recognition to regulation of social behaviour. Mol. Microbiol. 67, 241–253 (2008). · DOI · Paper page
  38. Martínez-Granero, F., Redondo-Nieto, M., Vesga, P., Martín, M. & Rivilla, R. AmrZ is a global transcriptional regulator implicated in iron uptake and environmental adaption in P. fluorescens F113. BMC Genomics 15, 237 (2014). · DOI
  39. Gur, E. & Sauer, R. T. Recognition of misfolded proteins by Lon, a AAA+ protease. Genes Dev. 22, 2267–2277 (2008). · DOI · Paper page
  40. Mukherjee, S. et al. Adaptor-mediated Lon proteolysis restricts Bacillus subtilis hyper flagellation. Proc. Natl Acad. Sci. USA 112, 250–255 (2015). · DOI
  41. Muriel, C. et al. The diguanylate cyclase AdrA regulates flagellar biosynthesis in Pseudomonas fluorescens F113 through SadB. Sci. Rep. 9, 1–9 (2019). · doi:10.1038/s41598-019-44554-z
  42. Ryder, C., Byrd, M. & Wozniak, D. J. Role of polysaccharides in Pseudomonas aeruginosa biofilm development. Curr. Opin. Microbiol. 10, 644–648 (2007). · DOI · Paper page
  43. Daniel, E. et al. ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization. BMC Bioinforma. 16, 303 (2015). · DOI
  44. Winsor, G. L. et al. Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database. Nucleic Acids Res. 44, D646–D653 (2016). · DOI · Paper page
  45. Anders, S., Pyl, P. T. & Huber, W. HTSeq-A Python framework to work with high-throughput sequencing data. Bioinformatics 31, 166–169 (2015). · DOI · Paper page
  46. Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012). · DOI · Paper page
  47. Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 1–21 (2014). · DOI · Paper page
  48. Barak, R. & Opatowsky, Y. Expression, derivatization, crystallization and experimental phasing of an extracellular segment of the human Robo1 receptor. Acta Crystallogr Sect. F. Struct. Biol. Cryst. Commun. 69, 771–775 (2013). · DOI
  49. Walter, T. S. et al. Lysine methylation as a routine rescue strategy for protein crystallization. Structure 14, 1617–1622 (2006). · DOI · Paper page
  50. Mueller-Dieckmann, C. et al. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility. Eur. Phys. J. 130, 70 (2015).
  51. Krug, M., Weiss, M. S., Heinemann, U. & Mueller, U. XDSAPP: a graphical user interface for the convenient processing of diffraction data using XDS. J. Appl Crystallogr 45, 568–572 (2012). · DOI · Paper page
  52. McCoy, A. J. et al. Phaser crystallographic software. J. Appl Crystallogr 40, 658–674 (2007). · DOI · Paper page
  53. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D. Biol. Crystallogr 66, 486–501 (2010). · DOI · Paper page
  54. Afonine, P. V. et al. Towards automated crystallographic structure refinement with phenix.refine. Acta Crystallogr D. Biol. Crystallogr 68, 352–367 (2012). · DOI · Paper page
  55. Joosten, R. P., Joosten, K., Murshudov, G. N. & Perrakis, A. PDB_REDO: constructive validation, more than just looking for errors. Acta Crystallogr D. Biol. Crystallogr 68, 484–496 (2012). · DOI · Paper page

Cited by

See all 6

Metadata

Authors
Ben-David, Y., Sporny, M., Brochin, Y., Piscon, B., Roth, S., Zander, I., Nisani, M., Shoshani, S., Yaron, O., Karako-Lampert, S., Lebenthal-Loinger, I., Danielli, A., Opatowsky, Y., & Banin, E.
Venue
npj Biofilms and Microbiomes
Published
2025-05-13
Volume
11
Issue
1
ISSN
2055-5008
Cited by
6
References
1
Language
en
Licence
Funders
Israel Science Foundation (1694/22 and 1125/17)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa. https://app.cochat.ai/papers/10.1038/s41522-025-00710-0
The paper
Ben-David, Y., Sporny, M., Brochin, Y., Piscon, B., Roth, S., Zander, I., Nisani, M., Shoshani, S., Yaron, O., Karako-Lampert, S., Lebenthal-Loinger, I., Danielli, A., Opatowsky, Y., & Banin, E. (2025). SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa. npj Biofilms and Microbiomes. https://doi.org/10.1038/s41522-025-00710-0

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.