The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes
Open accessAbstract
Extracellular vesicles (EVs) can mediate intercellular communication by transferring cargo proteins and nucleic acids between cells. The pathophysiological roles and clinical value of EVs are under intense investigation, yet most studies are limited by technical challenges in the isolation of nanoscale EVs (nEVs). Here, we report a lipid-nanoprobe system that enables spontaneous labelling of nEVs for subsequent magnetic enrichment in 15 minutes, with isolation efficiency and cargo composition similar to what can be achieved by the much slower and bulkier method of ultracentrifugation. We also show that this approach allows for downstream analyses of nucleic acids and proteins, enabling the identification of EGFR and KRAS mutations following nEV isolation from the blood plasma of non-small-cell lung-cancer patients. The efficiency and versatility of the lipid-nanoprobe approach opens up opportunities in point-of-care cancer diagnostics. Nanoscale extracellular vesicles can be efficiently isolated in about 15 minutes, for downstream analyses of nucleic acids and proteins, via spontaneous labelling through a lipid nanoprobe and subsequent magnetic capture of the labelled vesicles.
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Cites
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Cited by
Advances in therapeutic applications of extracellular vesicles
Wiklander, O. P. B., Brennan, M. Á., Lötvall, J., Breakefield, X. O., & EL Andaloussi, S. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav8521 · Cited by 1,039
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Metadata
- Authors
- Wan, Y., Cheng, G., Liu, X., Hao, S.-J., Nisic, M., Zhu, C.-D., Xia, Y.-Q., Li, W.-Q., Wang, Z.-G., Zhang, W.-L., Rice, S. J., Sebastian, A., Albert, I., Belani, C. P., & Zheng, S.-Y.
- Venue
- Nature Biomedical Engineering
- Published
- 2017-04-10
- Volume
- 1
- Issue
- 4
- ISSN
- 2157-846X (ISSN-L)
- OpenAlex
- W2607346246
- Cited by
- 279
- References
- 12
- FWCI
- 7.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Wan, Y., Cheng, G., Liu, X., Hao, S.-J., Nisic, M., Zhu, C.-D., Xia, Y.-Q., Li, W.-Q., Wang, Z.-G., Zhang, W.-L., Rice, S. J., Sebastian, A., Albert, I., Belani, C. P., & Zheng, S.-Y. (2017). Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-017-0058
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