Cited by 371Nanoparticle-Based Drug DeliveryMetal-Organic Frameworks: Synthesis and ApplicationsExtracellular vesicles in disease

Self-assembly of extracellular vesicle-like metal–organic framework nanoparticles for protection and intracellular delivery of biofunctional proteins

Cheng, Gong, Li, Wenqing, Ha, Laura, Han, Xiaohui, Hao, Sijie, Wan, Yuan, Wang, Zhigang, Dong, Fengping +3 more·2018·Journal of the American Chemical Society

Abstract

The intracellular delivery of biofunctional enzymes or therapeutic proteins through systemic administration is of great importance in therapeutic intervention of various diseases. However, current strategies face substantial challenges owing to various biological barriers, including susceptibility to protein degradation and denaturation, poor cellular uptake, and low transduction efficiency into the cytosol. Here, we developed a biomimetic nanoparticle platform for systemic and intracellular delivery of proteins. Through a biocompatible strategy, guest proteins are caged in the matrix of metal-organic frameworks (MOFs) with high efficiency (up to ∼94%) and high loading content up to ∼50 times those achieved by surface conjunction, and the nanoparticles were further decorated with the extracellular vesicle (EV) membrane with an efficiency as high as ∼97%. In vitro and in vivo study manifests that the EV-like nanoparticles can not only protect proteins against protease digestion and evade the immune system clearance but also selectively target homotypic tumor sites and promote tumor cell uptake and autonomous release of the guest protein after internalization. Assisted by biomimetic nanoparticles, intracellular delivery of the bioactive therapeutic protein gelonin significantly inhibits the tumor growth in vivo and increased 14-fold the therapeutic efficacy. Together, our work not only proposes a new concept to construct a biomimetic nanoplatform but also provides a new solution for systemic and intracellular delivery of protein.

Integrity signals

Registry

Crossref · 10.1021/jacs.8b03584

Cites

57 of 57 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Cheng, G., Li, W., Ha, L., Han, X., Hao, S., Wan, Y., Wang, Z., Dong, F., Zou, X., Mao, Y., & Zheng, S.-Y.
Affiliations
Pennsylvania State University (US)
Venue
Journal of the American Chemical Society
Published
2018-05-29
Volume
140
Issue
23
Pages
7282-7291
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
OpenAlex
W2806414351
Cited by
371
References
57
Citations by year
2026: 27 · 2025: 25 · 2024: 42 · 2023: 60 · 2022: 68 · 2021: 60 · 2020: 48 · 2019: 35 · 2018: 6
FWCI
12.05
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the American Chemical Society (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Cancer Institute (DP2 CA174508)
  • Huck Institute of Life Sciences, Pennsylvania State University
  • Materials Research Institute, Pennsylvania State University
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Organic Frameworks: Synthesis and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
metal-organic framework nanoparticles, biomimetic nanoparticles, intracellular protein delivery, gelonin, tumor-targeted delivery, immune evasion, protein encapsulation, tumor growth inhibition
MeSH terms
Extracellular Vesicles, Metal-Organic Frameworks, Animals, Antineoplastic Agents, Cell Membrane, Drug Carriers, Endocytosis, Humans, Neoplasms, Particle Size, Xenograft Model Antitumor Assays, Biomimetic Materials, Cell Line, Tumor, Mice, Nanoparticles, Ribosome Inactivating Proteins, Type 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review