Cited by 86AI in cancer detectionGenerative Adversarial Networks and Image SynthesisArtificial Intelligence in Healthcare and Education

A deep-learning model for transforming the style of tissue images from cryosectioned to formalin-fixed and paraffin-embedded

Ozyoruk, Kutsev Bengisu, Can, Sermet, Darbaz, Berkan, Başak, Kayhan, Demir, Derya, Gokceler, Guliz Irem, Serin, Gurdeniz, Hacisalihoglu, Uguray Payam +7 more·2022·Nature Biomedical Engineering

Abstract

Histological artefacts in cryosectioned tissue can hinder rapid diagnostic assessments during surgery. Formalin-fixed and paraffin-embedded (FFPE) tissue provides higher quality slides, but the process for obtaining them is laborious (typically lasting 12-48 h) and hence unsuitable for intra-operative use. Here we report the development and performance of a deep-learning model that improves the quality of cryosectioned whole-slide images by transforming them into the style of whole-slide FFPE tissue within minutes. The model consists of a generative adversarial network incorporating an attention mechanism that rectifies cryosection artefacts and a self-regularization constraint between the cryosectioned and FFPE images for the preservation of clinically relevant features. Transformed FFPE-style images of gliomas and of non-small-cell lung cancers from a dataset independent from that used to train the model improved the rates of accurate tumour subtyping by pathologists.

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Crossref · 10.1038/s41551-022-00952-9

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43 of 43 references verified0 citation errors · 0 not checkable

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  • Several studies have shown potential for AI to facilitate intraoperative cancer diagnosis using label-free and cryosectioned tissue samples.

    “several studies have demonstrated the potential of using AI to facilitate intraoperative diagnosis (e.g., during surgical resection) using label-free (40,41) and cryosectioned (42,43) tissue samples.”
  • Ozyoruk and colleagues demonstrated potential for AI to facilitate intraoperative subtyping of NSCLC by transforming cryosectioned tissue slides into the style of standard FFPE slides.

    “Ozyoruk and colleagues (43) also demonstrated the potential of using AI to facilitate intraoperative subtyping of NSCLC by transforming cryosectioned tissue slides into the style of standard formalin-fixed and paraffinembedded slides.”

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Authors
Ozyoruk, K. B., Can, S., Darbaz, B., Başak, K., Demir, D., Gokceler, G. I., Serin, G., Hacisalihoglu, U. P., Kurtuluş, E., Lu, M. Y., Chen, T. Y., Williamson, D. F. K., Yılmaz, F., Mahmood, F., & Turan, M.
Affiliations
  • Brigham and Women's Hospital (US)
  • Boğaziçi University (TR)
  • Dr Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi (TR)
  • Ege University (TR)
  • Stanford University (US)
  • Massachusetts Institute of Technology (US)
Corresponding author
  • Mahmood, Faisal
  • Turan, Mehmet
Venue
Nature Biomedical Engineering
Published
2022-12-23
Volume
6
Issue
12
Pages
1407-1419
ISSN
2157-846X (ISSN-L)
OpenAlex
W4312220066
Cited by
86
References
42
Citations by year
2026: 12 · 2025: 33 · 2024: 30 · 2023: 11
FWCI
6.09
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Biomedical Engineering (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Computer Science › Artificial Intelligence › AI in cancer detection
  • Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Generative Adversarial Networks and Image Synthesis
  • Health Sciences › Medicine › Health Informatics › Artificial Intelligence in Healthcare and Education
Keywords
formalin-fixed paraffin-embedded tissue, generative adversarial networks, attention mechanism, intraoperative histopathology, cancer subtyping, glioma, non-small cell lung cancer
MeSH terms
Deep Learning, Carcinoma, Non-Small-Cell Lung, Formaldehyde, Humans, Lung Neoplasms, Paraffin Embedding

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