A deep-learning model for transforming the style of tissue images from cryosectioned to formalin-fixed and paraffin-embedded
Abstract
Histological artefacts in cryosectioned tissue can hinder rapid diagnostic assessments during surgery. Formalin-fixed and paraffin-embedded (FFPE) tissue provides higher quality slides, but the process for obtaining them is laborious (typically lasting 12-48 h) and hence unsuitable for intra-operative use. Here we report the development and performance of a deep-learning model that improves the quality of cryosectioned whole-slide images by transforming them into the style of whole-slide FFPE tissue within minutes. The model consists of a generative adversarial network incorporating an attention mechanism that rectifies cryosection artefacts and a self-regularization constraint between the cryosectioned and FFPE images for the preservation of clinically relevant features. Transformed FFPE-style images of gliomas and of non-small-cell lung cancers from a dataset independent from that used to train the model improved the rates of accurate tumour subtyping by pathologists.
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Several studies have shown potential for AI to facilitate intraoperative cancer diagnosis using label-free and cryosectioned tissue samples.
“several studies have demonstrated the potential of using AI to facilitate intraoperative diagnosis (e.g., during surgical resection) using label-free (40,41) and cryosectioned (42,43) tissue samples.”
From Artificial Intelligence in Oncology: Current Landscape, Challenges, and Future Directions (2024) · cites this paper as [43]
Ozyoruk and colleagues demonstrated potential for AI to facilitate intraoperative subtyping of NSCLC by transforming cryosectioned tissue slides into the style of standard FFPE slides.
“Ozyoruk and colleagues (43) also demonstrated the potential of using AI to facilitate intraoperative subtyping of NSCLC by transforming cryosectioned tissue slides into the style of standard formalin-fixed and paraffinembedded slides.”
From Artificial Intelligence in Oncology: Current Landscape, Challenges, and Future Directions (2024) · cites this paper as [43]
Cites
Deep Residual Learning for Image Recognition
He, K., Zhang, X., Ren, S., & Sun, J. (2016) · 2016 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) · doi:10.1109/cvpr.2016.90 · Cited by 235,474 · see citing works
Unpaired image-to-image translation using cycle-consistent adversarial networks
Zhu, J.-Y., Park, T., Isola, P., & Efros, A. A. (2017) · 2017 IEEE International Conference on Computer Vision (ICCV) · doi:10.1109/iccv.2017.244 · Cited by 23,602 · see citing works
Image-to-Image Translation with Conditional Adversarial Networks
Isola, P., Zhu, J.-Y., Zhou, T., & Efros, A. A. (2017) · 2017 IEEE Conference on Computer Vision and Pattern Recognition (CVPR) · doi:10.1109/cvpr.2017.632 · Cited by 22,930 · see citing works
Statistical Methods for Rates and Proportions
Fleiss, J. L., Levin, B., & Paik, M. C. (2003) · Wiley Series in Probability and Statistics · doi:10.1002/0471445428 · Cited by 18,646 · see citing works
2017 IEEE Conference on Computer Vision and Pattern Recognition (CVPR)
(2017) · doi:10.1109/cvpr35066.2017 · Cited by 7,281 · see citing works
International Conference on Learning Representations (ICLR 2013)
松尾, 豊. (2013) · 人工知能学会誌 = Journal of Japanese Society for Artificial Intelligence · Cited by 6,286 · see citing works
“Least squares generative adversarial networks,”
Mao, X., Li, Q., Xie, H., Lau, R. Y. K., Wang, Z., & Smolley, S. P. (2017) · 2017 IEEE International Conference on Computer Vision (ICCV) · doi:10.1109/iccv.2017.304 · Cited by 5,437 · see citing works
2018 IEEE/CVF Conference on Computer Vision and Pattern Recognition
(2018) · doi:10.1109/cvpr40276.2018 · Cited by 4,769 · see citing works
Generative Adversarial Networks: An Overview
Creswell, A., White, T., Dumoulin, V., Arulkumaran, K., Sengupta, B., & Bharath, A. A. (2018) · IEEE Signal Processing Magazine · doi:10.1109/msp.2017.2765202 · Cited by 4,540 · see citing works
Unsupervised Feature Learning via Non-parametric Instance Discrimination
Wu, Z., Xiong, Y., Yu, S. X., & Lin, D. (2018) · 2018 IEEE/CVF Conference on Computer Vision and Pattern Recognition · doi:10.1109/cvpr.2018.00393 · Cited by 3,664 · see citing works
2017 IEEE International Conference on Computer Vision (ICCV)
(2017) · doi:10.1109/iccv37128.2017 · Cited by 3,129 · see citing works
Coudray, N., Ocampo, P. S., Sakellaropoulos, T., Narula, N., Snuderl, M., Fenyö, D., Moreira, A. L., Razavian, N., & Tsirigos, A. (2018) · Nature Medicine · doi:10.1038/s41591-018-0177-5 · Cited by 2,930 · see citing works
Clinical-grade computational pathology using weakly supervised deep learning on whole slide images
Campanella, G., Hanna, M. G., Geneslaw, L., Miraflor, A., Werneck Krauss Silva, V., Busam, K. J., Brogi, E., Reuter, V. E., Klimstra, D. S., & Fuchs, T. J. (2019) · Nature Medicine · doi:10.1038/s41591-019-0508-1 · Cited by 2,799 · see citing works
Multimodal Unsupervised Image-to-Image Translation
Huang, X., Liu, M.-Y., Belongie, S., & Kautz, J. (2018) · Lecture notes in computer science · doi:10.1007/978-3-030-01219-9_11 · Cited by 2,597 · see citing works
Data-efficient and weakly supervised computational pathology on whole-slide images
Lu, M. Y., Williamson, D. F. K., Chen, T. Y., Chen, R. J., Barbieri, M., & Mahmood, F. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-020-00682-w · Cited by 2,282 · see citing works
Self-citation2020 IEEE/CVF Conference on Computer Vision and Pattern Recognition (CVPR)
(2020) · doi:10.1109/cvpr42600.2020 · Cited by 1,805 · see citing works
Contrastive Learning for Unpaired Image-to-Image Translation
Park, T., Efros, A. A., Zhang, R., & Zhu, J.-Y. (2020) · Lecture notes in computer science · doi:10.1007/978-3-030-58545-7_19 · Cited by 1,605 · see citing works
Virtual histological staining of unlabelled tissue-autofluorescence images via deep learning
Rivenson, Y., Wang, H., Wei, Z., Haan, K. D., Zhang, Y., Wu, Y., Günaydın, H., Zuckerman, J. E., Chong, T., Sisk, A. E. J., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0362-y · Cited by 653 · see citing works
Chen, P.-H. C., Gadepalli, K., MacDonald, R., Liu, Y., Kadowaki, S., Nagpal, K., Kohlberger, T., Dean, J., Corrado, G. S., Hipp, J. D., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0539-7 · Cited by 342 · see citing works
Deep learning-based transformation of H&E stained tissues into special stains
Haan, K. D., Zhang, Y., Zuckerman, J. E., Liu, T., Sisk, A. E. J., Diaz, M. F. P., Jen, K., Nobori, A., Liou, S. S., Zhang, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25221-2 · Cited by 326 · see citing works
Geometry-Consistent Generative Adversarial Networks for One-Sided Unsupervised Domain Mapping
Fu, H., Gong, M., Wang, C.-H., Batmanghelich, K. N., Zhang, K., & Tao, D. (2019) · IEEE Computer Society Conference on Computer Vision and Pattern Recognition/Proceedings - IEEE Computer Society Conference on Computer Vision and Pattern Recognition/Proceedings · doi:10.1109/cvpr.2019.00253 · Cited by 261 · see citing works
Unsupervised Reverse Domain Adaptation for Synthetic Medical Images via Adversarial Training
Mahmood, F., Chen, R., & Durr, N. J. (2018) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2018.2842767 · Cited by 257 · see citing works
Self-citationAdversarial Stain Transfer for Histopathology Image Analysis
BenTaieb, A., & Hamarneh, G. (2017) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2017.2781228 · Cited by 231 · see citing works
A survey of methods for 3D histology reconstruction
Pichat, J., Iglesias, J. E., Yousry, T., Ourselin, S., & Modat, M. (2018) · Medical Image Analysis · doi:10.1016/j.media.2018.02.004 · Cited by 207 · see citing works
Sorin, V., Barash, Y., Konen, E., & Klang, E. (2020) · Academic Radiology · doi:10.1016/j.acra.2019.12.024 · Cited by 195 · see citing works
Oh, E., Choi, Y., Kwon, M. J., Kim, R. N., Kim, Y. J., Song, J., Jung, K. S., & Shin, Y. K. (2015) · PLoS ONE · doi:10.1371/journal.pone.0144162 · Cited by 115 · see citing works
TraVeLGAN: Image-To-Image Translation by Transformation Vector Learning
Amodio, M., & Krishnaswamy, S. (2019) · IEEE Computer Society Conference on Computer Vision and Pattern Recognition/Proceedings - IEEE Computer Society Conference on Computer Vision and Pattern Recognition/Proceedings · doi:10.1109/cvpr.2019.00919 · Cited by 110 · see citing works
Frozen Section Diagnoses of Small Pulmonary Nodules: Accuracy and Clinical Implications
Marchevsky, A. M., Changsri, C., Gupta, I., Fuller, C. B., Houck, W. V., & McKenna, R. J. (2004) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2004.05.003 · Cited by 109 · see citing works
Bittar, H. E. T., Incharoen, P., Althouse, A. D., & Đačić, S. (2015) · Modern Pathology · doi:10.1038/modpathol.2015.71 · Cited by 87 · see citing works
Turkish Population Structure and Genetic Ancestry Reveal Relatedness among Eurasian Populations
Hodoğlugil, U., & Mahley, R. W. (2012) · Annals of Human Genetics · doi:10.1111/j.1469-1809.2011.00701.x · Cited by 64 · see citing works
EndoL2H: Deep Super-Resolution for Capsule Endoscopy
Almalıoğlu, Y., Ozyoruk, K. B., Gökce, A., İncetan, K., Gokceler, G. I., Simsek, M. A., Ararat, K., Chen, R. J., Durr, N. J., Mahmood, F., et al. (2020) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2020.3016744 · Cited by 62 · see citing works
Self-citationClinical implications of positive margins following non‐small cell lung cancer surgery
Predina, J. D., Keating, J. J., Patel, N., Nims, S., & Singhal, S. K. (2015) · Journal of Surgical Oncology · doi:10.1002/jso.24130 · Cited by 45 · see citing works
Diagnostic Accuracy of Cytology Smear and Frozen Section in Glioma
Zin, A. A. M., & Zulkarnain, S. (2019) · Asian Pacific Journal of Cancer Prevention · doi:10.31557/apjcp.2019.20.2.321 · Cited by 44 · see citing works
Solheim, O. S., Tofte, K., Berger, C., & Torp, S. (2014) · Surgical Neurology International · doi:10.4103/2152-7806.146153 · Cited by 36 · see citing works
DeepLSR: a deep learning approach for laser speckle reduction
Bobrow, T. L., Mahmood, F., Inserni, M., & Durr, N. J. (2019) · Biomedical Optics Express · doi:10.1364/boe.10.002869 · Cited by 35 · see citing works
Self-citationAccuracy of frozen section analysis in correlation with surgical pathology diagnosis
Patil, P., Shukla, S., Bhake, A. S., & Hiwale, K. (2015) · International Journal of Research in Medical Sciences · doi:10.5455/2320-6012.ijrms20150203 · Cited by 31 · see citing works
Falahkheirkhah, K., Guo, T., Hwang, M. J., Tamboli, P., Wood, C. G., Karam, J. A., Sircar, K., & Bhargava, R. (2021) · Laboratory Investigation · doi:10.1038/s41374-021-00718-y · Cited by 20 · see citing works
Obeidat, F. N., Awad, H., Mansour, A. T., Hajeer, M., Al-jalabi, M. A., & Abudalu, L. (2018) · Turkish Neurosurgery · doi:10.5137/1019-5149.jtn.23220-18.2 · Cited by 19 · see citing works
Geometry-Consistent Generative Adversarial Networks for One-Sided Unsupervised Domain MappingPreprint
Fu, H., Gong, M., Wang, C., et al. (2018) · arXiv (Cornell University) · doi:10.48550/arxiv.1809.05852 · Cited by 18 · see citing works
On the acceptance of “fake” histopathology: A study on frozen sections optimized with deep learning
Siller, M., Stangassinger, L. M., Kreutzer, C., Boor, P., Bülow, R. D., Kraus, T. F. J., von Stillfried, S. F., Wolfl, S., Couillard‐Després, S., Oostingh, G. J., et al. (2022) · Journal of Pathology Informatics · doi:10.4103/jpi.jpi_53_21 · Cited by 13 · see citing works
An effective inflation treatment for frozen section diagnosis of small-sized lesions of the lung
Xiang, Z., Zhang, J., Zhao, J., Shao, J., Zhao, L., Zhang, Y., Qin, G., Xing, J., Han, Y., & Yu, K. (2020) · Journal of Thoracic Disease · doi:10.21037/jtd.2020.02.34 · Cited by 12 · see citing works
Renne, S. L., Redaelli, S., & Paolini, B. (2019) · Acta Histochemica · doi:10.1016/j.acthis.2019.05.002 · Cited by 6 · see citing works
Frozen Section Diagnosis of Pediatric Brain Tumors
Adesina, A. M. (2010) · Surgical pathology clinics · doi:10.1016/j.path.2010.07.001 · Cited by 5 · see citing works
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Towards a general-purpose foundation model for computational pathology
Chen, R. J., Ding, T., Lu, M. Y., Williamson, D. F. K., Jaume, G., Song, A. H., Chen, B., Zhang, A., Shao, D., Shaban, M., et al. (2024) · Nature Medicine · doi:10.1038/s41591-024-02857-3 · Cited by 1,560 · see citing works
Self-citationA whole-slide foundation model for digital pathology from real-world data
Xu, H., Usuyama, N., Bagga, J., Zhang, S., Rao, R., Naumann, T., Wong, C., Gero, Z., González, J., Gu, Y., et al. (2024) · Nature · doi:10.1038/s41586-024-07441-w · Cited by 921 · see citing works
Artificial intelligence for digital and computational pathology
Song, A. H., Jaume, G., Williamson, D. F. K., Lu, M. Y., Vaidya, A., Miller, T. R., & Mahmood, F. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00096-8 · Cited by 356 · see citing works
Self-citationAI-enabled organoids: Construction, analysis, and application
Bai, L., Wu, Y., Li, G., Zhang, W., Zhang, H., & Su, J. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.09.005 · Cited by 216 · see citing works
Artificial Intelligence in Oncology: Current Landscape, Challenges, and Future Directions
Lotter, W., Hassett, M. J., Schultz, N., Kehl, K. L., Van Allen, E. M., & Cerami, E. (2024) · Cancer Discovery · doi:10.1158/2159-8290.cd-23-1199 · Cited by 196 · see citing works
Rapid deep learning-assisted predictive diagnostics for point-of-care testing
Lee, S., Park, J., Woo, H., Yoo, Y. K., Lee, D., Chung, S. H., Yoon, D. S., Lee, K. B., & Lee, J. H. (2024) · Nature Communications · doi:10.1038/s41467-024-46069-2 · Cited by 123 · see citing works
Foundation models for fast, label-free detection of glioma infiltration
Kondepudi, A., Pekmezci, M., Hou, X., Scotford, K., Jiang, C., Rao, A. T., Harake, E. S., Chowdury, A., Al‐Holou, W. N., Wang, L., et al. (2024) · Nature · doi:10.1038/s41586-024-08169-3 · Cited by 119 · see citing works
Analysis of 3D pathology samples using weakly supervised AI
Song, A. H., Williams, M., Williamson, D. F. K., Chow, S. S. L., Jaume, G., Gao, G., Zhang, A., Chen, B., Baras, A. S., Serafin, R. J., et al. (2024) · Cell · doi:10.1016/j.cell.2024.03.035 · Cited by 108 · see citing works
Self-citationPati, P., Karkampouna, S., Bonollo, F., Compérat, É., Radić, M., Spahn, M., Martinelli, A. L., Wartenberg, M., Julio, M. K., & Rapsomaniki, M. (2024) · Nature Machine Intelligence · doi:10.1038/s42256-024-00889-5 · Cited by 93 · see citing works
Liu, Z., Chen, L., Cheng, H., Ao, J., Xiong, J., Liu, X., Chen, Y., Mao, Y. Q., & Ji, M. (2024) · Science Advances · doi:10.1126/sciadv.adn3426 · Cited by 83 · see citing works
Machine learning in computational histopathology: Challenges and opportunities
Cooper, M. C., Ji, Z., & Krishnan, R. G. (2023) · Genes Chromosomes and Cancer · doi:10.1002/gcc.23177 · Cited by 81 · see citing works
Machine learning methods for histopathological image analysis: updates in 2024
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Progress and challenges of artificial intelligence in lung cancer clinical translation
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Brodsky, V., Ullah, E., Bychkov, A., Song, A. H., Walk, E. E., Louis, P., Rasool, G., Singh, R. S., Mahmood, F., Bui, M. M., et al. (2025) · Archives of Pathology & Laboratory Medicine · doi:10.5858/arpa.2024-0215-ra · Cited by 48 · see citing works
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Mi, H., Sivagnanam, S., Ho, W. J., Zhang, S., Bergman, D. R., Deshpande, A. M., Baras, A. S., Jaffee, E. M., Coussens, L. M., Fertig, E. J., et al. (2024) · Briefings in Bioinformatics · doi:10.1093/bib/bbae421 · Cited by 38 · see citing works
Fu, X., Sahai, E. A., & Wilkins, A. C. (2023) · The Journal of Pathology · doi:10.1002/path.6153 · Cited by 36 · see citing works
Zhang, X., Xiao, Z., Ma, J., Wu, X., Zhao, J., Zhang, S., Li, R., Pan, Y. F., & Liu, J. (2025) · IEEE Transactions on Image Processing · doi:10.1109/tip.2025.3551128 · Cited by 32 · see citing works
Altini, N., Marvulli, T. M., Zito, F. A., Caputo, M., Tommasi, S., Azzariti, A., Brunetti, A., Prencipe, B., Mattioli, E., Summa, S. D., et al. (2023) · Computer Methods and Programs in Biomedicine · doi:10.1016/j.cmpb.2023.107511 · Cited by 29 · see citing works
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Cazzaniga, G., Rossi, M., Eccher, A., Girolami, I., L’Imperio, V., Nguyen, H. V., Becker, J. U., Bueno, G., Sbaraglia, M., Tos, A. P. D., et al. (2023) · Journal of Nephrology · doi:10.1007/s40620-023-01775-w · Cited by 21 · see citing works
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Li, H., Du, Z., Zhu, L., Zhang, C., Xiong, J., Zhou, B., Dong, B., Zhang, X., & Alifu, N. (2024) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.4c04748 · Cited by 21 · see citing works
Virtual staining for pathology: Challenges, limitations and perspectives
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Yan, B., Wen, Z., Xue, L., Wang, T., Liu, Z., Long, W., Li, Y., & Jing, R. (2025) · International Journal of Oral Science · doi:10.1038/s41368-025-00346-y · Cited by 18 · see citing works
Başak, K., Ozyoruk, K. B., & Demir, D. (2023) · Turkish Journal of Pathology · doi:10.5146/tjpath.2023.01601 · Cited by 18 · see citing works
Self-citationZhang, X., Zhao, Z., Wang, R., Chen, H., Zheng, X., Liu, L., Lan, L., Li, P., Wu, S., Cao, Q., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-48171-x · Cited by 17 · see citing works
Roetzer-Pejrimovsky, T., Nenning, K., Kiesel, B., Klughammer, J., Rajchl, M., Baumann, B., Langs, G., & Wöehrer, A. (2024) · GigaScience · doi:10.1093/gigascience/giae057 · Cited by 16 · see citing works
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Fang, Z., Wang, Y., Xie, P., Wang, Z., & Zhang, Y. (2024) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2024.3520129 · Cited by 12 · see citing works
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Jensen, M. P., Qiang, Z., Khan, D. Z., Stoyanov, D. V., Baldeweg, S. E., Jaunmuktane, Z., Brandner, S., & Marcus, H. J. (2024) · Neuropathology and Applied Neurobiology · doi:10.1111/nan.12981 · Cited by 11 · see citing works
Zhang, W., Hui, T. H., Tse, P. Y., Hill, F., Lau, C., & Li, X. (2024) · Lecture notes in computer science · doi:10.1007/978-3-031-72083-3_17 · Cited by 11 · see citing works
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Cheng, S., Chang, S., Li, Y., Novoseltseva, A., Lin, S., Wu, Y., Zhu, J., McKee, A. C., Rosene, D. L., Wang, H., et al. (2025) · Light Science & Applications · doi:10.1038/s41377-024-01658-0 · Cited by 8 · see citing works
Lee, Y., Liu, X., Hao, M., Liu, T., & Regev, A. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.10.627865 · Cited by 7 · see citing works
Zhao, L., Zhao, W., Qiu, L., Jiang, M., Qian, L., Ting, H.-N., Fu, X., Zhang, P., Han, Y., & Zhao, J. (2024) · Biomedical Signal Processing and Control · doi:10.1016/j.bspc.2024.107097 · Cited by 7 · see citing works
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Ho, M. M., Dubey, S., Chong, Y., Knudsen, B. S., & Taşdizen, T. (2025) · IEEE Winter Conference on Applications of Computer Vision · doi:10.1109/wacv61041.2025.00430 · Cited by 6 · see citing works
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Liu, X., Sun, T., Chen, H., Wu, S., Cheng, H., Liu, X., Lai, Q., Wang, K., Chen, L., Lu, J., et al. (2025) · Modern Pathology · doi:10.1016/j.modpat.2025.100749 · Cited by 5 · see citing works
His‐MMDM: Multi‐Domain and Multi‐Omics Translation of Histopathological Images with Diffusion Models
Li, Z., Su, T., Zhang, B., Han, W., Zhang, S., Sun, G., Cong, Y., Chen, X., Qi, J., Wang, Y., et al. (2026) · Advanced Science · doi:10.1002/advs.202518066 · Cited by 5 · see citing works
Kılıç, K. D. (2023) · Advances in computational intelligence and robotics book series · doi:10.4018/978-1-6684-9814-9.ch008 · Cited by 5 · see citing works
Pati, P., Karkampouna, S., Bonollo, F., Compérat, É., Radić, M., Spahn, M., Martinelli, A. L., Wartenberg, M., Julio, M. K., & Rapsomaniki, M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.29.568996 · Cited by 5 · see citing works
Ozyoruk, K. B., Harmon, S. A., Lay, N. S., Yılmaz, E. C., Bağcı, U., Citrin, D. E., Wood, B. J., Pinto, P., Choyke, P. L., & Türkbey, B. (2024) · Journal of Personalized Medicine · doi:10.3390/jpm14101047 · Cited by 4 · see citing works
Self-citationLu, Y., Peng, X., Yin, Z., Fan, X., Fan, J., Mi, Y., & Li, G. (2026) · Microchimica Acta · doi:10.1007/s00604-026-08054-y · Cited by 4 · see citing works
Generative Adversarial Networks for Stain Normalisation in Histopathology
Breen, J., Zucker, K., Allen, K., Ravikumar, N., & Orsi, N. M. (2024) · Springer eBooks · doi:10.1007/978-3-031-46238-2_11 · Cited by 4 · see citing works
Koyama, Y., Nakao, R., Sato, J., Honda, M., Inamori, O., Tanaka, N., Morinaga, Y., Konishi, E., Harada, Y., Tanaka, H., et al. (2025) · ACTA HISTOCHEMICA ET CYTOCHEMICA · doi:10.1267/ahc.25-00007 · Cited by 3 · see citing works
A Multi-Perspective Self-Supervised Generative Adversarial Network for FS to FFPE Stain Transfer
Lin, Y., Wang, Y., Fang, Z., Li, Z., Guan, X., Jiang, D., & Zhang, Y. (2024) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2024.3460795 · Cited by 3 · see citing works
Universal NIR-II fluorescence image enhancement via square and square root network
Yu, X., Shi, X., Tian, J., & Hu, Z. (2024) · Signal Processing · doi:10.1016/j.sigpro.2024.109818 · Cited by 3 · see citing works
Jiang, C., Li, M., Zheng, C., Yan, S., Kong, L., Wu, Y., Zhang, J., Chao, X., Cai, X., Feng, W., et al. (2025) · Laboratory Investigation · doi:10.1016/j.labinv.2025.104134 · Cited by 3 · see citing works
Correlated Multiple IHC Virtual Staining for Breast Histopathological Images
Guan, X., Zhang, Z., Wang, Y., Zhang, Y., Jiang, D., & Zhang, Y. (2025) · IEEE International Conference on Acoustics Speech and Signal Processing · doi:10.1109/icassp49660.2025.10889239 · Cited by 2 · see citing works
NIR ‐ II Fluorescence Image Translation via Latent Space Disentanglement
Yu, X., Tian, J., & Hu, Z. (2025) · International Journal of Imaging Systems and Technology · doi:10.1002/ima.70028 · Cited by 2 · see citing works
Tan, X., & Tan, L. (2023) · Transactions on Computer Science and Intelligent Systems Research · doi:10.62051/2z2ngm94 · Cited by 2 · see citing works
Künstliche Intelligenz in der Pathologie: Status quo und Zukunftsperspektiven
Foersch, S., & Schulz, S. (2024) · Die Onkologie · doi:10.1007/s00761-024-01482-6 · Cited by 2 · see citing works
Universal NIR-II fluorescence image enhancement via covariance weighted attention network
Yu, X., Tian, J., & Hu, Z. (2024) · Multimedia Systems · doi:10.1007/s00530-024-01533-3 · Cited by 1 · see citing works
NIR-II fluorescence image enhancement via multi-step modulation
Yu, X., Tian, J., & Hu, Z. (2025) · Expert Systems with Applications · doi:10.1016/j.eswa.2025.128728 · Cited by 1 · see citing works
PRINTER:Deformation-Aware Adversarial Learning for Virtual IHC Staining with In Situ Fidelity
Yuan, Y., Xue, B., Pu, B., Wang, C., & Jin, C. (2025) · ACM International Conference on Multimedia (ACM MM) · doi:10.1145/3746027.3755487 · Cited by 1 · see citing works
MCS-Stain: Boosting FFPE-to-HE Virtual Staining With Multiple Cell Semantics
Hu, Y., Du, Z., Lin, W., Yang, S., Yu, L., Zhang, G., & Wang, L. (2025) · IEEE Transactions on Medical Imaging · doi:10.1109/tmi.2025.3628174 · Cited by 1 · see citing works
Kulkarni, R., Maddox, A., Bailey, S., & Rao, A. (2026) · npj Artificial Intelligence · doi:10.1038/s44387-025-00050-6 · Cited by 1 · see citing works
Integration of artificial intelligence in histopathological and radiological image analysis
Alkhalaf, A. K., Alkhareeb, S. A., & Alshammari, M. M. (2024) · International Journal of Health Sciences · doi:10.53730/ijhs.v8ns1.15010 · Cited by 1 · see citing works
Patkar, S., Rosean, T. R., Patel, P. G., Harmon, S. A., Choyke, P. L., Jamaspishvili, T., & Türkbey, B. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.11.658673 · Cited by 2 · see citing works
Künstliche Intelligenz in der Pathologie: Status quo und Zukunftsperspektiven
Foersch, S., & Schulz, S. (2024) · best practice onkologie · doi:10.1007/s11654-024-00572-6
Leveraging Pre-trained Models for FF-to-FFPE Histopathological Image Translation
Zhang, Q., Li, J., Liao, P., Hu, J., Guan, T., Han, A., & He, Y. (2024) · IEEE International Conference on Bioinformatics and Biomedicine (BIBM) · doi:10.1109/bibm62325.2024.10822745
Learning content and style representation for NIR-II fluorescence image translation
Yu, X., Shi, X., Tian, J., & Hu, Z. (2025) · Signal Image and Video Processing · doi:10.1007/s11760-025-04075-x
Chen, Q., Wang, Z., Xia, L., Shi, Y., Xu, B., Chai, J., Zhang, T., & Wang, C. (2025) · medRxiv · doi:10.1101/2025.04.13.25323912
Xue, C., Wu, Y., Cai, X., He, J., Zheng, C., Li, M., Luo, R., Song, L., Li, X., Feng, W., et al. (2025) · Breast Cancer Research · doi:10.1186/s13058-025-02064-z
Yoshai, E., Goldinger, G., Kogan, T., Zakharov, A., Haifler, M., & Shaked, N. T. (2025) · Scientific Reports · doi:10.1038/s41598-025-12181-6
Coherent Raman scattering microscopy for cancer diagnosis
Ji, M. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-22253-5.00012-1
Lu, Y., Peng, X., Yin, Z. Y., Fan, X., Fan, J., Mi, Y., & Li, G. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.22.683875
Yuan, Y., Yue, Q., Wang, Y., Du, Z., Wang, X., Yan, F., Cheng, H., Chen, K., Wu, Z., Song, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-66853-y
Liao, I. Y., Fadlelbari, M. M. S., Khattab, M., Ahmed, A. A., & Pan, J. W. (2025) · ACM International Conference on Multimedia in Asia (MMAsia) · doi:10.1145/3743093.3770927
Intraoperative biopsy imaging of lung cancer risk
Qian, S., Yang, L., Meng, J., Zhou, L., Han, T., Chen, L., Xi, G., Jiang, R., Wang, C., Niu, B., et al. (2026) · Communications Medicine · doi:10.1038/s43856-026-01406-y
Diagnosis of Lung Diseases in the Artificial Intelligence Era
Papavassiliou, K. A., Sofianidi, A. A., & Papavassiliou, A. G. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27041796
Cai, L., Jiang, S., Liang, J., Liu, F., Zhang, B., Reitsam, N. G., Zeng, Q., Hu, Z., Ma, Y., Li, Z., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.02.25.707931
100-Second Large-Area Chemical Imaging by Integrated Four-Channel CARS Microscopy
Ju, W., Huang, Z., Li, S., Zhong, Y., Li, Y., Li, N., Yang, C., Huang, Y., Wang, S., Yan, S., et al. (2026) · Chemical & Biomedical Imaging · doi:10.1021/cbmi.6c00016
Fusing imaging and metabolic modeling via multimodal deep learning in ovarian cancer
Eftekhari, N., Verma, S., Saha, A., Zampieri, G., Sawan, S., Occhipinti, A., & Angione, C. (2026) · Cell Systems · doi:10.1016/j.cels.2026.101594
Xstainer: A Novel Virtual Staining Tool Powered by Advanced Deep Learning Techniques
Kinali, F. N., Demir, D., Özyörük, K. B., Almalıoğlu, Y., Köse, E. Ç., Williamson, D. F. K., Serın, G., Keles, G. E., Kumbaracı, B. S., Utku, C., et al. (2026) · Advanced Intelligent Systems · doi:10.1002/aisy.70424
Self-citationArtificial intelligence-enabled organoids and organ-on-a-chip for pioneering biomedical platforms
Zhao, Y., Xie, D., Liu, H., Peng, B., Li, X., Wang, X., Leng, Y., Liu, J., Yang, T., Li, H., et al. (2026) · Bio-Design and Manufacturing · doi:10.1631/bdm.2500394
Zhang, Y., Liu, Q., Zhao, Z., Chen, Q., Zheng, S., & Bai, X. (2026) · Biomedical Signal Processing and Control · doi:10.1016/j.bspc.2026.111384
Zhou, W., Zhou, D., Zhang, Q., Zang, P., Jiang, G., Cong, F., Liang, C., Li, S., Lu, C., & Xu, H. (2026) · Information Fusion · doi:10.1016/j.inffus.2026.104879
- Authors
- Ozyoruk, K. B., Can, S., Darbaz, B., Başak, K., Demir, D., Gokceler, G. I., Serin, G., Hacisalihoglu, U. P., Kurtuluş, E., Lu, M. Y., Chen, T. Y., Williamson, D. F. K., Yılmaz, F., Mahmood, F., & Turan, M.
- Affiliations
- Brigham and Women's Hospital (US)
- Boğaziçi University (TR)
- Dr Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi (TR)
- Ege University (TR)
- Stanford University (US)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Mahmood, Faisal
- Turan, Mehmet
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2022-12-23
- Volume
- 6
- Issue
- 12
- Pages
- 1407-1419
- ISSN
- 2157-846X (ISSN-L)
- OpenAlex
- W4312220066
- Cited by
- 86
- References
- 42
- Citations by year
- 2026: 12 · 2025: 33 · 2024: 30 · 2023: 11
- FWCI
- 6.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Computer Science › Artificial Intelligence › AI in cancer detection
- Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Generative Adversarial Networks and Image Synthesis
- Health Sciences › Medicine › Health Informatics › Artificial Intelligence in Healthcare and Education
- Keywords
- formalin-fixed paraffin-embedded tissue, generative adversarial networks, attention mechanism, intraoperative histopathology, cancer subtyping, glioma, non-small cell lung cancer
- MeSH terms
- Deep Learning, Carcinoma, Non-Small-Cell Lung, Formaldehyde, Humans, Lung Neoplasms, Paraffin Embedding
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